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Title: Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants

Fat storage-inducing transmembrane protein 2 (FIT2) is an endoplasmic reticulum (ER)-localized protein that plays an important role in lipid droplet (LD) formation in animal cells. However, no obvious homologue of FIT2 is found in plants. We tested the function of FIT2 in plant cells by ectopically expressing mouse (Mus musculus) FIT2 in Nicotiana tabacum suspension-cultured cells, Nicotiana benthamiana leaves and Arabidopsis thaliana plants. Confocal microscopy indicated that the expression of FIT2 dramatically increased the number and size of LDs in leaves of N. benthamiana and Arabidopsis, and lipidomics analysis and mass spectrometry imaging confirmed the accumulation of neutral lipids in leaves. FIT2 also increased seed oil content by ~13% in some stable, overexpressing lines of Arabidopsis. Furthermore, when expressed transiently in leaves of N. benthamiana or suspension cells of N. tabacum, FIT2 localized specifically to the ER and was often concentrated at certain regions of the ER that resembled ER-LD junction sites. FIT2 also colocalized at the ER with other proteins known to be involved in triacylglycerol biosynthesis or LD formation in plants, but not with ER resident proteins involved in electron transfer or ERvesicle exit sites. Collectively, these results demonstrate that mouse FIT2 promotes LD accumulation in plants, amore » surprising functional conservation in the context of a plant cell given the apparent lack of FIT2 homologues in higher plants. Our results suggest also that FIT2 expression represents an effective synthetic biology strategy for elaborating neutral lipid compartments in plant tissues for potential biofuel or bioproduct purposes.« less
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1] ;  [2] ;  [3] ;  [1]
  1. Univ. of North Texas, Denton, TX (United States). Center for Plant Lipid Research
  2. Univ. of Guelph, ON (Canada). Dept. of Molecular and Cellular Biology
  3. US Dept. of Agriculture (USDA-ARS)., Maricopa, AZ (United States). US Arid-Land Agricultural Research Center
Publication Date:
Grant/Contract Number:
SC0016536; FG02-09ER64812
Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 15; Journal Issue: 7; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Research Org:
Univ. of North Texas, Denton, TX (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Natural Sciences and Engineering Research Council of Canada (NSERC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; endoplasmic reticulum; fat storage-inducing transmembrane protein 2; lipid droplets; lipid storage; triacylglycerol; lipid partitioning
OSTI Identifier:
1339585
Alternate Identifier(s):
OSTI ID: 1339586; OSTI ID: 1374537

Cai, Yingqi, McClinchie, Elizabeth, Price, Ann, Nguyen, Thuy N., Gidda, Satinder K., Watt, Samantha C., Yurchenko, Olga, Park, Sunjung, Sturtevant, Drew, Mullen, Robert T., Dyer, John M., and Chapman, Kent D.. Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants. United States: N. p., Web. doi:10.1111/pbi.12678.
Cai, Yingqi, McClinchie, Elizabeth, Price, Ann, Nguyen, Thuy N., Gidda, Satinder K., Watt, Samantha C., Yurchenko, Olga, Park, Sunjung, Sturtevant, Drew, Mullen, Robert T., Dyer, John M., & Chapman, Kent D.. Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants. United States. doi:10.1111/pbi.12678.
Cai, Yingqi, McClinchie, Elizabeth, Price, Ann, Nguyen, Thuy N., Gidda, Satinder K., Watt, Samantha C., Yurchenko, Olga, Park, Sunjung, Sturtevant, Drew, Mullen, Robert T., Dyer, John M., and Chapman, Kent D.. 2017. "Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants". United States. doi:10.1111/pbi.12678.
@article{osti_1339585,
title = {Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants},
author = {Cai, Yingqi and McClinchie, Elizabeth and Price, Ann and Nguyen, Thuy N. and Gidda, Satinder K. and Watt, Samantha C. and Yurchenko, Olga and Park, Sunjung and Sturtevant, Drew and Mullen, Robert T. and Dyer, John M. and Chapman, Kent D.},
abstractNote = {Fat storage-inducing transmembrane protein 2 (FIT2) is an endoplasmic reticulum (ER)-localized protein that plays an important role in lipid droplet (LD) formation in animal cells. However, no obvious homologue of FIT2 is found in plants. We tested the function of FIT2 in plant cells by ectopically expressing mouse (Mus musculus) FIT2 in Nicotiana tabacum suspension-cultured cells, Nicotiana benthamiana leaves and Arabidopsis thaliana plants. Confocal microscopy indicated that the expression of FIT2 dramatically increased the number and size of LDs in leaves of N. benthamiana and Arabidopsis, and lipidomics analysis and mass spectrometry imaging confirmed the accumulation of neutral lipids in leaves. FIT2 also increased seed oil content by ~13% in some stable, overexpressing lines of Arabidopsis. Furthermore, when expressed transiently in leaves of N. benthamiana or suspension cells of N. tabacum, FIT2 localized specifically to the ER and was often concentrated at certain regions of the ER that resembled ER-LD junction sites. FIT2 also colocalized at the ER with other proteins known to be involved in triacylglycerol biosynthesis or LD formation in plants, but not with ER resident proteins involved in electron transfer or ERvesicle exit sites. Collectively, these results demonstrate that mouse FIT2 promotes LD accumulation in plants, a surprising functional conservation in the context of a plant cell given the apparent lack of FIT2 homologues in higher plants. Our results suggest also that FIT2 expression represents an effective synthetic biology strategy for elaborating neutral lipid compartments in plant tissues for potential biofuel or bioproduct purposes.},
doi = {10.1111/pbi.12678},
journal = {Plant Biotechnology Journal},
number = 7,
volume = 15,
place = {United States},
year = {2017},
month = {1}
}

Works referenced in this record:

The α/β Hydrolase CGI-58 and Peroxisomal Transport Protein PXA1 Coregulate Lipid Homeostasis and Signaling in Arabidopsis
journal, May 2013
  • Park, Sunjung; Gidda, Satinder K.; James, Christopher N.
  • The Plant Cell, Vol. 25, Issue 5, p. 1726-1739
  • DOI: 10.1105/tpc.113.111898

Compartmentation of Triacylglycerol Accumulation in Plants
journal, November 2011
  • Chapman, Kent D.; Ohlrogge, John B.
  • Journal of Biological Chemistry, Vol. 287, Issue 4, p. 2288-2294
  • DOI: 10.1074/jbc.R111.290072

A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
journal, September 2003
  • Curtis, Mark D.; Grossniklaus, Ueli
  • Plant Physiology, Vol. 133, Issue 2, p. 462-469
  • DOI: 10.1104/pp.103.027979

Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipid droplet accumulation in plants
journal, September 2010
  • James, Christopher N.; Horn, Patrick J.; Case, Charlene R.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17833-17838
  • DOI: 10.1073/pnas.0911359107

A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962

Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998

Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
journal, September 2009

Tobacco as a production platform for biofuel overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass
journal, April 2010