Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation
Abstract
Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant– fungal–endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations and allosteric effectors. Such multimodal regulations may be a general mechanism for metabolic modulation.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Duke Univ., Durham, NC (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1339384
- Alternate Identifier(s):
- OSTI ID: 1399551
- Grant/Contract Number:
- AC05-00OR22725; SC10010566
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; proteomics; metabolomics; fungus; endobacterium; symbiotic metabolism; multimodal regulation
Citation Formats
Li, Zhou, Yao, Qiuming, Dearth, Stephen P., Entler, Matthew R., Castro Gonzalez, Hector F., Uehling, Jessie K., Vilgalys, Rytas J., Hurst, Gregory B., Campagna, Shawn R., Labbé, Jessy L., and Pan, Chongle. Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation. United States: N. p., 2016.
Web. doi:10.1111/1462-2920.13605.
Li, Zhou, Yao, Qiuming, Dearth, Stephen P., Entler, Matthew R., Castro Gonzalez, Hector F., Uehling, Jessie K., Vilgalys, Rytas J., Hurst, Gregory B., Campagna, Shawn R., Labbé, Jessy L., & Pan, Chongle. Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation. United States. https://doi.org/10.1111/1462-2920.13605
Li, Zhou, Yao, Qiuming, Dearth, Stephen P., Entler, Matthew R., Castro Gonzalez, Hector F., Uehling, Jessie K., Vilgalys, Rytas J., Hurst, Gregory B., Campagna, Shawn R., Labbé, Jessy L., and Pan, Chongle. Mon .
"Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation". United States. https://doi.org/10.1111/1462-2920.13605. https://www.osti.gov/servlets/purl/1339384.
@article{osti_1339384,
title = {Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation},
author = {Li, Zhou and Yao, Qiuming and Dearth, Stephen P. and Entler, Matthew R. and Castro Gonzalez, Hector F. and Uehling, Jessie K. and Vilgalys, Rytas J. and Hurst, Gregory B. and Campagna, Shawn R. and Labbé, Jessy L. and Pan, Chongle},
abstractNote = {Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant– fungal–endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations and allosteric effectors. Such multimodal regulations may be a general mechanism for metabolic modulation.},
doi = {10.1111/1462-2920.13605},
journal = {Environmental Microbiology},
number = 1,
volume = 12,
place = {United States},
year = {Mon Nov 21 00:00:00 EST 2016},
month = {Mon Nov 21 00:00:00 EST 2016}
}
Web of Science
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