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Title: Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1

The fundamental biophysics of gliding microtubule (MT) motility by surface-tethered kinesin-1 motor proteins has been widely studied, as well as applied to capture and transport analytes in bioanalytical microdevices. In these systems, phenomena such as molecular wear and fracture into shorter MTs have been reported due the mechanical forces applied on the MT during transport. In the present work, we show that MTs can be split longitudinally into protofilament bundles (PFBs) by the work performed by surface-bound kinesin motors. We examine the properties of these PFBs using several techniques (e.g., fluorescence microscopy, SEM, AFM), and show that the PFBs continue to be mobile on the surface and display very high curvature compared to MT. Further, higher surface density of kinesin motors and shorter kinesin-surface tethers promote PFB formation, whereas modifying MT with GMPCPP or higher paclitaxel concentrations did not affect PFB formation.
Authors:
 [1] ;  [2] ;  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies
Publication Date:
Report Number(s):
SAND2017-0052J
Journal ID: ISSN 2045-2322; srep39408
Grant/Contract Number:
AC04-94AL85000; AC52-06NA25396; SC0001035
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1339254

VanDelinder, Virginia, Adams, Peter G., and Bachand, George D.. Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1. United States: N. p., Web. doi:10.1038/srep39408.
VanDelinder, Virginia, Adams, Peter G., & Bachand, George D.. Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1. United States. doi:10.1038/srep39408.
VanDelinder, Virginia, Adams, Peter G., and Bachand, George D.. 2016. "Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1". United States. doi:10.1038/srep39408. https://www.osti.gov/servlets/purl/1339254.
@article{osti_1339254,
title = {Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1},
author = {VanDelinder, Virginia and Adams, Peter G. and Bachand, George D.},
abstractNote = {The fundamental biophysics of gliding microtubule (MT) motility by surface-tethered kinesin-1 motor proteins has been widely studied, as well as applied to capture and transport analytes in bioanalytical microdevices. In these systems, phenomena such as molecular wear and fracture into shorter MTs have been reported due the mechanical forces applied on the MT during transport. In the present work, we show that MTs can be split longitudinally into protofilament bundles (PFBs) by the work performed by surface-bound kinesin motors. We examine the properties of these PFBs using several techniques (e.g., fluorescence microscopy, SEM, AFM), and show that the PFBs continue to be mobile on the surface and display very high curvature compared to MT. Further, higher surface density of kinesin motors and shorter kinesin-surface tethers promote PFB formation, whereas modifying MT with GMPCPP or higher paclitaxel concentrations did not affect PFB formation.},
doi = {10.1038/srep39408},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {12}
}