skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

Authors:
 [1];  [2];  [2];  [2];  [1];  [1]
  1. Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska, Lincoln NE USA
  2. Department of Plant Biology and UC Davis Genome Center, University of California, Davis CA USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1339021
Alternate Identifier(s):
OSTI ID: 1352473
Grant/Contract Number:  
SC0012459; 1444612; 13-39385; 01133480
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Name: Plant Biotechnology Journal Journal Volume: 15 Journal Issue: 5; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., and Weeks, Donald P. Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing. United Kingdom: N. p., 2017. Web. doi:10.1111/pbi.12663.
Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., & Weeks, Donald P. Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing. United Kingdom. doi:10.1111/pbi.12663.
Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., and Weeks, Donald P. Thu . "Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing". United Kingdom. doi:10.1111/pbi.12663.
@article{osti_1339021,
title = {Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing},
author = {Jiang, Wen Zhi and Henry, Isabelle M. and Lynagh, Peter G. and Comai, Luca and Cahoon, Edgar B. and Weeks, Donald P.},
abstractNote = {},
doi = {10.1111/pbi.12663},
journal = {Plant Biotechnology Journal},
number = 5,
volume = 15,
place = {United Kingdom},
year = {2017},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.12663

Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Genetic variation of six desaturase genes in flax and their impact on fatty acid composition
journal, August 2013

  • Thambugala, Dinushika; Duguid, Scott; Loewen, Evelyn
  • Theoretical and Applied Genetics, Vol. 126, Issue 10
  • DOI: 10.1007/s00122-013-2161-2

Modification of Seed Oil Composition in Arabidopsis by Artificial microRNA-Mediated Gene Silencing
journal, January 2012

  • Belide, Srinivas; Petrie, James Robertson; Shrestha, Pushkar
  • Frontiers in Plant Science, Vol. 3
  • DOI: 10.3389/fpls.2012.00168

Identification of three genes encoding microsomal oleate desaturases (FAD2) from the oilseed crop Camelina sativa
journal, February 2011


Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis
journal, February 2014

  • Feng, Z.; Mao, Y.; Xu, N.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 12
  • DOI: 10.1073/pnas.1400822111

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012


Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes
journal, January 2010


Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil
journal, April 2012

  • Pham, Anh-Tung; Shannon, J. Grover; Bilyeu, Kristin D.
  • Theoretical and Applied Genetics, Vol. 125, Issue 3
  • DOI: 10.1007/s00122-012-1849-z

A high-oleic-acid and low-palmitic-acid soybean: agronomic performance and evaluation as a feedstock for biodiesel
journal, June 2009


Genetic and Epigenetic Aspects of Polyploid Evolution in Plants
journal, January 2013

  • Madlung, A.; Wendel, J. F.
  • Cytogenetic and Genome Research, Vol. 140, Issue 2-4
  • DOI: 10.1159/000351430

Genome edited sheep and cattle
journal, September 2014


Extensive Recombination Due to Heteroduplexes Generates Large Amounts of Artificial Gene Fragments during PCR
journal, September 2014


Evaluation of Camelina sativa oil as a feedstock for biodiesel production
journal, January 2005


Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content: Figure 1.
journal, September 2009


FAD2 and FAD3 Desaturases Form Heterodimers That Facilitate Metabolic Channeling in Vivo
journal, May 2014

  • Lou, Ying; Schwender, Jorg; Shanklin, John
  • Journal of Biological Chemistry, Vol. 289, Issue 26
  • DOI: 10.1074/jbc.M114.572883

Transgenic trait deployment using designed nucleases
journal, August 2015

  • Petolino, Joseph F.; Kumar, Sandeep
  • Plant Biotechnology Journal, Vol. 14, Issue 2
  • DOI: 10.1111/pbi.12457

Transgenic expression of a Δ12-epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid
journal, April 2001


Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013

  • Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram
  • Plant Biotechnology Journal, Vol. 11, Issue 6
  • DOI: 10.1111/pbi.12068

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family
journal, May 2014

  • Haun, William; Coffman, Andrew; Clasen, Benjamin M.
  • Plant Biotechnology Journal, Vol. 12, Issue 7
  • DOI: 10.1111/pbi.12201

Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos
journal, October 1999

  • Cahoon, E. B.; Carlson, T. J.; Ripp, K. G.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 22
  • DOI: 10.1073/pnas.96.22.12935

The advantages and disadvantages of being polyploid
journal, October 2005

  • Comai, Luca
  • Nature Reviews Genetics, Vol. 6, Issue 11
  • DOI: 10.1038/nrg1711

A Cas9-guide RNA complex preorganized for target DNA recognition
journal, June 2015


A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri
journal, January 1998


Oil-seed crop: Camelina sativa
journal, May 1997


Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007


RNA-Guided Human Genome Engineering via Cas9
journal, January 2013


High-efficiency TALEN-based gene editing produces disease-resistant rice
journal, May 2012

  • Li, Ting; Liu, Bo; Spalding, Martin H.
  • Nature Biotechnology, Vol. 30, Issue 5
  • DOI: 10.1038/nbt.2199

Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013


Transgenic cotton plants with increased seed oleic acid content
journal, September 2001

  • Chapman, Kent D.; Austin-Brown, Shea; Sparace, Salvatore A.
  • Journal of the American Oil Chemists' Society, Vol. 78, Issue 9
  • DOI: 10.1007/s11746-001-0368-y

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System
journal, December 2015


Genome Evolution Due to Allopolyploidization in Wheat
journal, November 2012


Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
journal, August 2013

  • Jiang, Wenzhi; Zhou, Huanbin; Bi, Honghao
  • Nucleic Acids Research, Vol. 41, Issue 20
  • DOI: 10.1093/nar/gkt780

Molecular scissors and their application in genetically modified farm animals
journal, January 2015


Reduced Oil Accumulation in Cottonseeds Transformed with a Nonfunctional Allele of a Delta-12 Fatty Acid Desaturase ()
journal, January 2008


The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus
journal, September 2013


Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis
journal, September 2015

  • Mao, Yanfei; Zhang, Zhengjing; Feng, Zhengyan
  • Plant Biotechnology Journal, Vol. 14, Issue 2
  • DOI: 10.1111/pbi.12468

Use of designer nucleases for targeted gene and genome editing in plants
journal, August 2015

  • Weeks, Donald P.; Spalding, Martin H.; Yang, Bing
  • Plant Biotechnology Journal, Vol. 14, Issue 2
  • DOI: 10.1111/pbi.12448