Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

Jiang, Wen Zhi; Henry, Isabelle M.; Lynagh, Peter G.; Comai, Luca; Cahoon, Edgar B.; Weeks, Donald P.

doi:10.1111/pbi.12663

Title: Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

Authors:

Jiang, Wen Zhi ^[1]; Henry, Isabelle M. ^[2]; Lynagh, Peter G. ^[2]; Comai, Luca ^[2]; Cahoon, Edgar B. ^[1]; Weeks, Donald P. ^[1]

Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska, Lincoln NE USA
Department of Plant Biology and UC Davis Genome Center, University of California, Davis CA USA

Publication Date:: Thu Jan 12 00:00:00 EST 2017

Sponsoring Org.:: USDOE

OSTI Identifier:: 1339021

Alternate Identifier(s):: OSTI ID: 1352473

Grant/Contract Number:: SC0012459; 1444612; 13-39385; 01133480

Resource Type:: Published Article

Journal Name:: Plant Biotechnology Journal

Additional Journal Information:: Journal Name: Plant Biotechnology Journal Journal Volume: 15 Journal Issue: 5; Journal ID: ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied Biology

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., and Weeks, Donald P. Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing.  United Kingdom: N. p., 2017. 
Web.  doi:10.1111/pbi.12663.

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                    Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., & Weeks, Donald P. Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing.  United Kingdom.  https://doi.org/10.1111/pbi.12663

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                    Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., and Weeks, Donald P. Thu .  
"Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing".  United Kingdom.  https://doi.org/10.1111/pbi.12663.

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@article{osti_1339021,

  title        = {Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing},

  author       = {Jiang, Wen Zhi and Henry, Isabelle M. and Lynagh, Peter G. and Comai, Luca and Cahoon, Edgar B. and Weeks, Donald P.},

  abstractNote = {},

  doi          = {10.1111/pbi.12663},

  journal      = {Plant Biotechnology Journal},

  number       = 5,

  volume       = 15,

  place        = {United Kingdom},

  year         = {Thu Jan 12 00:00:00 EST 2017},

  month        = {Thu Jan 12 00:00:00 EST 2017}

}

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Title: Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

Citation Formats

Identification of functional BrFAD2-1 gene encoding microsomal delta-12 fatty acid desaturase from Brassica rapa and development of Brassica napus containing high oleic acid contents journal, June 2011

Genetic variation of six desaturase genes in flax and their impact on fatty acid composition journal, August 2013

Modification of Seed Oil Composition in Arabidopsis by Artificial microRNA-Mediated Gene Silencing journal, January 2012

Identification of three genes encoding microsomal oleate desaturases (FAD2) from the oilseed crop Camelina sativa journal, February 2011

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles journal, October 2014

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis journal, February 2014

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity journal, June 2012

Camelina: An Emerging Oilseed Platform for Advanced Biofuels and Bio-Based Materials book, November 2013

Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes journal, January 2010

Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil journal, April 2012

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation journal, July 2015

A high-oleic-acid and low-palmitic-acid soybean: agronomic performance and evaluation as a feedstock for biodiesel journal, June 2009

Genetic and Epigenetic Aspects of Polyploid Evolution in Plants journal, January 2013

Genome edited sheep and cattle journal, September 2014

Extensive Recombination Due to Heteroduplexes Generates Large Amounts of Artificial Gene Fragments during PCR journal, September 2014

Evaluation of Camelina sativa oil as a feedstock for biodiesel production journal, January 2005

Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content: Figure 1. journal, September 2009

FAD2 and FAD3 Desaturases Form Heterodimers That Facilitate Metabolic Channeling in Vivo journal, May 2014

Transgenic trait deployment using designed nucleases journal, August 2015

Transgenic expression of a Δ12-epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid journal, April 2001

Camelina seed transcriptome: a tool for meal and oil improvement and translational research journal, April 2013

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family journal, May 2014

Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos journal, October 1999

The advantages and disadvantages of being polyploid journal, October 2005

A Cas9-guide RNA complex preorganized for target DNA recognition journal, June 2015

A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri journal, January 1998

Oil-seed crop: Camelina sativa journal, May 1997

Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation journal, September 2007

RNA-Guided Human Genome Engineering via Cas9 journal, January 2013

High-efficiency TALEN-based gene editing produces disease-resistant rice journal, May 2012

Multiplex Genome Engineering Using CRISPR/Cas Systems journal, January 2013

Transgenic cotton plants with increased seed oleic acid content journal, September 2001

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System journal, December 2015

Genome Evolution Due to Allopolyploidization in Wheat journal, November 2012

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice journal, August 2013

Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations journal, June 2014

Molecular scissors and their application in genetically modified farm animals journal, January 2015

Reduced Oil Accumulation in Cottonseeds Transformed with a Nonfunctional Allele of a Delta-12 Fatty Acid Desaturase () journal, January 2008

The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus journal, September 2013

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis journal, September 2015

Use of designer nucleases for targeted gene and genome editing in plants journal, August 2015

Accumulation of Ricinoleic, Lesquerolic, and Densipolic Acids in Seeds of Transgenic Arabidopsis Plants That Express a Fatty Acyl Hydroxylase cDNA from Castor Bean journal, March 1997

Identification of functional BrFAD2-1 gene encoding microsomal delta-12 fatty acid desaturase from Brassica rapa and development of Brassica napus containing high oleic acid contents
journal, June 2011

Genetic variation of six desaturase genes in flax and their impact on fatty acid composition
journal, August 2013

Modification of Seed Oil Composition in Arabidopsis by Artificial microRNA-Mediated Gene Silencing
journal, January 2012

Identification of three genes encoding microsomal oleate desaturases (FAD2) from the oilseed crop Camelina sativa
journal, February 2011

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles
journal, October 2014

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis
journal, February 2014

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012

Camelina: An Emerging Oilseed Platform for Advanced Biofuels and Bio-Based Materials
book, November 2013

Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes
journal, January 2010

Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil
journal, April 2012

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
journal, July 2015

A high-oleic-acid and low-palmitic-acid soybean: agronomic performance and evaluation as a feedstock for biodiesel
journal, June 2009

Genetic and Epigenetic Aspects of Polyploid Evolution in Plants
journal, January 2013

Genome edited sheep and cattle
journal, September 2014

Extensive Recombination Due to Heteroduplexes Generates Large Amounts of Artificial Gene Fragments during PCR
journal, September 2014

Evaluation of Camelina sativa oil as a feedstock for biodiesel production
journal, January 2005

Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content: Figure 1.
journal, September 2009

FAD2 and FAD3 Desaturases Form Heterodimers That Facilitate Metabolic Channeling in Vivo
journal, May 2014

Transgenic trait deployment using designed nucleases
journal, August 2015

Transgenic expression of a Δ12-epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid
journal, April 2001

Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family
journal, May 2014

Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos
journal, October 1999

The advantages and disadvantages of being polyploid
journal, October 2005

A Cas9-guide RNA complex preorganized for target DNA recognition
journal, June 2015

A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri
journal, January 1998

Oil-seed crop: Camelina sativa
journal, May 1997

Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007

RNA-Guided Human Genome Engineering via Cas9
journal, January 2013

High-efficiency TALEN-based gene editing produces disease-resistant rice
journal, May 2012

Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013

Transgenic cotton plants with increased seed oleic acid content
journal, September 2001

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System
journal, December 2015

Genome Evolution Due to Allopolyploidization in Wheat
journal, November 2012

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
journal, August 2013

Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations
journal, June 2014

Molecular scissors and their application in genetically modified farm animals
journal, January 2015

Reduced Oil Accumulation in Cottonseeds Transformed with a Nonfunctional Allele of a Delta-12 Fatty Acid Desaturase ()
journal, January 2008

The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus
journal, September 2013

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis
journal, September 2015

Use of designer nucleases for targeted gene and genome editing in plants
journal, August 2015

Accumulation of Ricinoleic, Lesquerolic, and Densipolic Acids in Seeds of Transgenic Arabidopsis Plants That Express a Fatty Acyl Hydroxylase cDNA from Castor Bean
journal, March 1997