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Title: Procedures for cryogenic X-ray ptychographic imaging of biological samples

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This paper describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [5] ;  [5] ;  [3] ;  [6]
  1. Univ. College London, London (United Kingdom). London Centre for Nanotechnology; Research Complex at Harwell, Oxfordshire (England). Rutherford Appleton Lab.; Brunel Univ. London, Uxbridge (England)
  2. Univ. College London, London (United Kingdom). London Centre for Nanotechnology; Research Complex at Harwell, Oxfordshire (England). Rutherford Appleton Lab.; Southern Univ. of Science and Technology, Shenzhen (China)
  3. Univ. College London, London (United Kingdom). London Centre for Nanotechnology; Research Complex at Harwell, Oxfordshire (England). Rutherford Appleton Lab.
  4. Research Complex at Harwell, Oxfordshire (England). Rutherford Appleton Lab.
  5. Diamond Light Source, Didcot, Oxfordshire (England)
  6. Univ. College London, London (United Kingdom). London Centre for Nanotechnology; Research Complex at Harwell, Oxfordshire (England). Rutherford Appleton Lab.; Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.
Publication Date:
Report Number(s):
BNL-113441-2017-JA
Journal ID: ISSN 2052-2525; IUCRAJ; R&D Project: PO011; KC0201060; TRN: US1700989
Grant/Contract Number:
SC0012704; BB/ H022597/1
Type:
Published Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 4; Journal Issue: 2; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; human chromosomes; cell nuclei; chromosome imaging; X-ray ptychography; plunge freezing
OSTI Identifier:
1339020
Alternate Identifier(s):
OSTI ID: 1342640