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Title: Transgenic switchgrass ( Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression

Five different types of transgenic ( GAUT4, miRNA, MYB4, COMT and FPGS) Panicum virgatum L. (switchgrass) were grown in a field in Knoxville, Tenn., USA over two consecutive years between 2011 and 2015 in separate experiments. Clonal replicates were established (year-one) and produced much greater biomass during the second year. After each growing season the above ground biomass was analyzed for cell wall sugars and for recalcitrance to enzymatic digestibility, and biofuel using a separate hydrolysis and fermentation (SHF) screen. Here, each transgenic event and control had more glucan, xylan and less ethanol (g/g basis) from the second year of growth relative to the first year plants. There was no correlation between plant carbohydrate content and biofuel production. In each of cell wall-targeted transgenics, GAUT4, MYB4, COMT and FPGS, the second year of growth resulted in increased carbohydrate abundance (up to 12%) and reduced recalcitrance through higher ethanol yields (up to 21%) over the non-transgenic control plants.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [4] ;  [4] ;  [5] ;  [4] ;  [4] ;  [2] ;  [3] ;  [4] ;  [5] ;  [1] ;  [1] more »;  [1] « less
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of North Texas, Denton, TX (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); The Samuel Roberts Noble Foundation, Ardmore, OK (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Name: Plant Biotechnology Journal; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1338528

Dumitrache, Alexandru, Natzke, Jace, Rodriguez, Jr., Miguel, Yee, Kelsey L., Thompson, Olivia A., Poovaiah, Charleson R., Shen, Hui, Mazarei, Mitra, Baxter, Holly L., Fu, Chunxiang, Wang, Zeng -Yu, Biswal, Ajaya K., Li, Guifen, Tang, Yuhong, Stewart, Jr., C. Neal, Dixon, Richard A., Nelson, Richard S., Mohnen, Debra, Mielenz, Jonathan, Brown, Steven D., and Davison, Brian H.. Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression. United States: N. p., Web. doi:10.1111/pbi.12666.
Dumitrache, Alexandru, Natzke, Jace, Rodriguez, Jr., Miguel, Yee, Kelsey L., Thompson, Olivia A., Poovaiah, Charleson R., Shen, Hui, Mazarei, Mitra, Baxter, Holly L., Fu, Chunxiang, Wang, Zeng -Yu, Biswal, Ajaya K., Li, Guifen, Tang, Yuhong, Stewart, Jr., C. Neal, Dixon, Richard A., Nelson, Richard S., Mohnen, Debra, Mielenz, Jonathan, Brown, Steven D., & Davison, Brian H.. Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression. United States. doi:10.1111/pbi.12666.
Dumitrache, Alexandru, Natzke, Jace, Rodriguez, Jr., Miguel, Yee, Kelsey L., Thompson, Olivia A., Poovaiah, Charleson R., Shen, Hui, Mazarei, Mitra, Baxter, Holly L., Fu, Chunxiang, Wang, Zeng -Yu, Biswal, Ajaya K., Li, Guifen, Tang, Yuhong, Stewart, Jr., C. Neal, Dixon, Richard A., Nelson, Richard S., Mohnen, Debra, Mielenz, Jonathan, Brown, Steven D., and Davison, Brian H.. 2016. "Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression". United States. doi:10.1111/pbi.12666. https://www.osti.gov/servlets/purl/1338528.
@article{osti_1338528,
title = {Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: A two-year comparative analysis of field-grown lines modified for target gene or genetic element expression},
author = {Dumitrache, Alexandru and Natzke, Jace and Rodriguez, Jr., Miguel and Yee, Kelsey L. and Thompson, Olivia A. and Poovaiah, Charleson R. and Shen, Hui and Mazarei, Mitra and Baxter, Holly L. and Fu, Chunxiang and Wang, Zeng -Yu and Biswal, Ajaya K. and Li, Guifen and Tang, Yuhong and Stewart, Jr., C. Neal and Dixon, Richard A. and Nelson, Richard S. and Mohnen, Debra and Mielenz, Jonathan and Brown, Steven D. and Davison, Brian H.},
abstractNote = {Five different types of transgenic (GAUT4, miRNA, MYB4, COMT and FPGS) Panicum virgatum L. (switchgrass) were grown in a field in Knoxville, Tenn., USA over two consecutive years between 2011 and 2015 in separate experiments. Clonal replicates were established (year-one) and produced much greater biomass during the second year. After each growing season the above ground biomass was analyzed for cell wall sugars and for recalcitrance to enzymatic digestibility, and biofuel using a separate hydrolysis and fermentation (SHF) screen. Here, each transgenic event and control had more glucan, xylan and less ethanol (g/g basis) from the second year of growth relative to the first year plants. There was no correlation between plant carbohydrate content and biofuel production. In each of cell wall-targeted transgenics, GAUT4, MYB4, COMT and FPGS, the second year of growth resulted in increased carbohydrate abundance (up to 12%) and reduced recalcitrance through higher ethanol yields (up to 21%) over the non-transgenic control plants.},
doi = {10.1111/pbi.12666},
journal = {Plant Biotechnology Journal},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {11}
}

Works referenced in this record:

Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Loss of function of folylpolyglutamate synthetase 1 reduces lignin content and improves cell wall digestibility in Arabidopsis
journal, December 2015
  • Srivastava, Avinash C.; Chen, Fang; Ray, Tui
  • Biotechnology for Biofuels, Vol. 8, Article No. 224
  • DOI: 10.1186/s13068-015-0403-z