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Title: Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a widely adopted, versatile technique, especially in high-throughput analysis and imaging. However, matrix-dependent selectivity of analytes is often a severe limitation. In this work, a mixture of organic 2,5-dihydroxybenzoic acid and inorganic Fe 3O 4 nanoparticles is developed as a binary MALDI matrix to alleviate the well-known issue of triacylglycerol (TG) ion suppression by phosphatidylcholine (PC). In application to lipid standards and maize seed cross-sections, the binary matrix not only dramatically reduced the ion suppression of TG, but also efficiently desorbed and ionized a wide variety of lipids such as cationic PC, anionic phosphatidylethanolamine (PE) and phosphatidylinositol (PI), and neutral digalactosyldiacylglycerol (DGDG). The binary matrix was also very efficient for large polysaccharides, which were not detected by either of the individual matrices. As a result, the usefulness of the binary matrix is demonstrated in MS imaging of maize seed sections, successfully visualizing diverse medium-size molecules and acquiring high-quality MS/MS spectra for these compounds.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [1] ;  [2]
  1. Iowa State Univ., Ames, IA (United States)
  2. (United States)
Publication Date:
Report Number(s):
IS-J-9044
Journal ID: ISSN 2046-2069; RSCACL
Grant/Contract Number:
AC02-07CH11358
Type:
Accepted Manuscript
Journal Name:
RSC Advances
Additional Journal Information:
Journal Volume: 6; Journal Issue: 101; Journal ID: ISSN 2046-2069
Publisher:
Royal Society of Chemistry
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1337660

Feenstra, Adam D., Ames Lab., Ames, IA, O'Neill, Kelly C., Yagnik, Gargey B., Ames Lab., Ames, IA, Lee, Young Jin, and Ames Lab., Ames, IA. Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides. United States: N. p., Web. doi:10.1039/C6RA20469D.
Feenstra, Adam D., Ames Lab., Ames, IA, O'Neill, Kelly C., Yagnik, Gargey B., Ames Lab., Ames, IA, Lee, Young Jin, & Ames Lab., Ames, IA. Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides. United States. doi:10.1039/C6RA20469D.
Feenstra, Adam D., Ames Lab., Ames, IA, O'Neill, Kelly C., Yagnik, Gargey B., Ames Lab., Ames, IA, Lee, Young Jin, and Ames Lab., Ames, IA. 2016. "Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides". United States. doi:10.1039/C6RA20469D. https://www.osti.gov/servlets/purl/1337660.
@article{osti_1337660,
title = {Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides},
author = {Feenstra, Adam D. and Ames Lab., Ames, IA and O'Neill, Kelly C. and Yagnik, Gargey B. and Ames Lab., Ames, IA and Lee, Young Jin and Ames Lab., Ames, IA},
abstractNote = {Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a widely adopted, versatile technique, especially in high-throughput analysis and imaging. However, matrix-dependent selectivity of analytes is often a severe limitation. In this work, a mixture of organic 2,5-dihydroxybenzoic acid and inorganic Fe3O4 nanoparticles is developed as a binary MALDI matrix to alleviate the well-known issue of triacylglycerol (TG) ion suppression by phosphatidylcholine (PC). In application to lipid standards and maize seed cross-sections, the binary matrix not only dramatically reduced the ion suppression of TG, but also efficiently desorbed and ionized a wide variety of lipids such as cationic PC, anionic phosphatidylethanolamine (PE) and phosphatidylinositol (PI), and neutral digalactosyldiacylglycerol (DGDG). The binary matrix was also very efficient for large polysaccharides, which were not detected by either of the individual matrices. As a result, the usefulness of the binary matrix is demonstrated in MS imaging of maize seed sections, successfully visualizing diverse medium-size molecules and acquiring high-quality MS/MS spectra for these compounds.},
doi = {10.1039/C6RA20469D},
journal = {RSC Advances},
number = 101,
volume = 6,
place = {United States},
year = {2016},
month = {10}
}