Title: Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a widely adopted, versatile technique, especially in high-throughput analysis and imaging. However, matrix-dependent selectivity of analytes is often a severe limitation. In this work, a mixture of organic 2,5-dihydroxybenzoic acid and inorganic Fe₃O₄ nanoparticles is developed as a binary MALDI matrix to alleviate the well-known issue of triacylglycerol (TG) ion suppression by phosphatidylcholine (PC). In application to lipid standards and maize seed cross-sections, the binary matrix not only dramatically reduced the ion suppression of TG, but also efficiently desorbed and ionized a wide variety of lipids such as cationic PC, anionic phosphatidylethanolamine (PE) and phosphatidylinositol (PI), and neutral digalactosyldiacylglycerol (DGDG). The binary matrix was also very efficient for large polysaccharides, which were not detected by either of the individual matrices. As a result, the usefulness of the binary matrix is demonstrated in MS imaging of maize seed sections, successfully visualizing diverse medium-size molecules and acquiring high-quality MS/MS spectra for these compounds.

Authors:

Feenstra, Adam D. ^[1]; Ames Lab., Ames, IA ^[2]; O'Neill, Kelly C. ^[1]; Yagnik, Gargey B. ^[1]; Ames Lab., Ames, IA ^[2]; Lee, Young Jin ^[1]; Ames Lab., Ames, IA ^[2]

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+ Show Author Affiliations

1. Iowa State Univ., Ames, IA (United States)
2. (United States)

Publication Date:

Thu Oct 13 00:00:00 EDT 2016

Research Org.:

Ames Lab., Ames, IA (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:

1337660

Report Number(s):

IS-J-9044
Journal ID: ISSN 2046-2069; RSCACL

Grant/Contract Number:  

AC02-07CH11358

Resource Type:

Accepted Manuscript

Journal Name:

RSC Advances

Additional Journal Information:

Journal Volume: 6; Journal Issue: 101; Journal ID: ISSN 2046-2069

Publisher:

Royal Society of Chemistry

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES