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Title: Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi

Abstract

Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. This last approach is shown to provide a novel method for identifying wood oxidation. When lignin was mildly oxidized, the association between AO and lignin was reduced such that stained wood sections emitted less green light during fluorescence microscopy. This change was detectable after less than a week, an interval that past work has shown to be too short for significant delignification of wood. Although fungal hyphae were observed in only a few wood lumina, oxidation was widespread, appearing relatively uniform over regions several hundred micrometers from the hyphae. As a result, this observation suggests that both classes of fungi release low molecular weight mild oxidants during the first few days of colonization.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
USDA Forest Service, Madison, WI (United States). Forest Products Lab.
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1337469
Alternate Identifier(s):
OSTI ID: 1362292
Grant/Contract Number:  
AI02-07ER64491; SC0006929
Resource Type:
Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Name: PLoS ONE Journal Volume: 11 Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science (PLoS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., Hunt, Christopher G., and Berrin, ed., Jean-Guy. Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0159715.
Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., Hunt, Christopher G., & Berrin, ed., Jean-Guy. Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi. United States. doi:10.1371/journal.pone.0159715.
Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., Hunt, Christopher G., and Berrin, ed., Jean-Guy. Mon . "Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi". United States. doi:10.1371/journal.pone.0159715.
@article{osti_1337469,
title = {Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi},
author = {Houtman, Carl J. and Kitin, Peter and Houtman, Jon C. D. and Hammel, Kenneth E. and Hunt, Christopher G. and Berrin, ed., Jean-Guy},
abstractNote = {Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. This last approach is shown to provide a novel method for identifying wood oxidation. When lignin was mildly oxidized, the association between AO and lignin was reduced such that stained wood sections emitted less green light during fluorescence microscopy. This change was detectable after less than a week, an interval that past work has shown to be too short for significant delignification of wood. Although fungal hyphae were observed in only a few wood lumina, oxidation was widespread, appearing relatively uniform over regions several hundred micrometers from the hyphae. As a result, this observation suggests that both classes of fungi release low molecular weight mild oxidants during the first few days of colonization.},
doi = {10.1371/journal.pone.0159715},
journal = {PLoS ONE},
number = 7,
volume = 11,
place = {United States},
year = {2016},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1371/journal.pone.0159715

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Cited by: 2 works
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Works referenced in this record:

Photochemistry on surfaces: Fluorescence emission of monomers and dimers and triplet state absorption of acridine orange adsorbed on microcrystalline cellulose
journal, February 1992

  • Wilkinson, F.; Worrall, D. R.; Ferreira, L. F. Vieira
  • Spectrochimica Acta Part A: Molecular Spectroscopy, Vol. 48, Issue 2
  • DOI: 10.1016/0584-8539(92)80015-O

Distribution of acidic and esterified polygalacturonans in sapwood of spruce, birch and aspen
journal, December 2001


Wood decay under the microscope
journal, November 2007


Acridine orange as an indicator of the cytoplasmic ribosome state
journal, November 1997


Fluorescence Lifetime Measurements and Biological Imaging
journal, May 2010

  • Berezin, Mikhail Y.; Achilefu, Samuel
  • Chemical Reviews, Vol. 110, Issue 5
  • DOI: 10.1021/cr900343z

A Highly Diastereoselective Oxidant Contributes to Ligninolysis by the White Rot Basidiomycete Ceriporiopsis subvermispora
journal, September 2014

  • Yelle, Daniel J.; Kapich, Alexander N.; Houtman, Carl J.
  • Applied and Environmental Microbiology, Vol. 80, Issue 24
  • DOI: 10.1128/AEM.02111-14

A method to determine the charge demand of single pulp fibers
journal, December 2004


Removal of Wood Components During Chlorite Delignification of Black Spruce
journal, April 1971

  • Ahlgren, P. A.; Goring, D. A. I.
  • Canadian Journal of Chemistry, Vol. 49, Issue 8
  • DOI: 10.1139/v71-207

13 C NMR Spectroscopic Study of Spruce Lignin Degraded Phanerochaete Chrysosporium
journal, January 1982


Thermodynamic behaviour of acridine orange in solution. Model system for studying stacking and charge-effects on self-aggregation
journal, January 1973

  • Robinson, B. H.; Löffler, A.; Schwarz, G.
  • Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases, Vol. 69, Issue 0
  • DOI: 10.1039/f19736900056

Kinetic Studies on Acridine Orange-DNA Interaction
journal, May 1972


Critical comparison and validation of methods for determination of anionic groups in pulp fibres
journal, August 2002


Beiträge zur fluoreszenzmikroskopischen Darstellung des Zellinhaltes, insbesondere des Cytoplasmas und des Zellkerns
journal, January 1940


Role of fungal peroxidases in biological ligninolysis
journal, June 2008


Cell wall alterations in loblolly pine wood decayed by the white-rot fungus, Ceriporiopsis subvermispora
journal, March 1997


Proposed supramolecular structure of lignin in softwood tracheid compound middle lamella regions
journal, December 2012

  • Terashima, Noritsugu; Yoshida, Masato; Hafrén, Jonas
  • Holzforschung, Vol. 66, Issue 8
  • DOI: 10.1515/hf-2012-0021

Peroxyl radicals are potential agents of lignin biodegradation
journal, November 1999


Selective staining as a tool for wood fibre characterization
journal, December 2005


Safranine fluorescent staining of wood cell walls
journal, January 2008


1H NMR investigation of self-association of aromatic drug molecules in aqueous solution. Structural and thermodynamical analysis
journal, January 1996

  • Davies, David B.; Djimant, Leonid N.; Veselkov, Alexei N.
  • Journal of the Chemical Society, Faraday Transactions, Vol. 92, Issue 3
  • DOI: 10.1039/ft9969200383

Fluorescent Labeling of Lignin in the Wood Pulp Fiber Wall
journal, January 2005

  • Li, Kecheng; Reeve, Douglas W.
  • Journal of Wood Chemistry and Technology, Vol. 24, Issue 2
  • DOI: 10.1081/WCT-200026572

Luminescence of the solid complexes of acridine orange with RNA
journal, January 1982

  • Kapuscinski, Jan; Darzynkiewicz, Zbigniew; Melamed, Myron R.
  • Cytometry, Vol. 2, Issue 4
  • DOI: 10.1002/cyto.990020402

Acridine orange as a fluorescent probe for the study of polyelectrolyte complexes
journal, March 1979


Lignin selective dyes: quantum-mechanical study of their characteristics
journal, June 2011


Isothermal titration calorimetry: A thermodynamic interpretation of measurements
journal, December 2012

  • Grolier, Jean-Pierre E.; del Río, José Manuel
  • The Journal of Chemical Thermodynamics, Vol. 55
  • DOI: 10.1016/j.jct.2012.05.018

Interactions of the DNA intercalator acridine orange, with itself, with caffeine, and with double stranded DNA
journal, April 2002


Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi
journal, June 2014

  • Riley, Robert; Salamov, Asaf A.; Brown, Daren W.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 27
  • DOI: 10.1073/pnas.1400592111

Fluoreszenzmikrokopische Beobachtungen an pilzbefallenem Holz
journal, February 1968

  • von Aufsess, H.; von Pechmann, H.; Graessle, E.
  • Holz als Roh- und Werkstoff, Vol. 26, Issue 2
  • DOI: 10.1007/BF02615810