Binary Electroosmotic-Pump Nanoflow Gradient Generator for Miniaturized High-Performance Liquid Chromatography
Abstract
High-performance liquid chromatography (HPLC) plays an important role in biotechnology, and a majority of chromatographic separations use gradient elution. While gradient generators can be built in different formats, binary pumps or quaternary pumps are most frequently used for gradient generator constructions. We have recently developed a high-pressure electroosmotic pump (EOP); the pump can be manufactured at a cost of a few hundred dollars. However, it is challenging to use this pump to deliver a gradient eluent directly. In this study, we first improve the monolith preparation by applying a pressure to the monomer solution during polymerization. We assemble a binary EOP gradient generator and discuss the relationship between the gradient profile and voltage applied to the EOP. Here we demonstrate the feasibility of the binary EOP gradient generator for generating a smooth and reproducible nanoflow gradient. After integration of the gradient generator into a miniaturized HPLC system, we use the HPLC system for separating peptide mixtures from trypsin-digested proteins. Lastly, the performance comparison between the above miniaturized HPLC system and an Agilent 1200 HPLC system exhibits comparable efficiencies, resolutions, and peak capacities.
- Authors:
-
- Lanzhou Univ. (China). College of Chemistry and Chemical Engineering
- Univ. of Oklahoma, Norman, OK (United States). Dept. of Chemistry and Biochemistry
- Publication Date:
- Research Org.:
- MicroChem Solutions, Norman, OK (United States)
- Sponsoring Org.:
- USDOE Advanced Research Projects Agency - Energy (ARPA-E); Oklahoma Center for the Advancement of Science and Technology; National Natural Science Foundation of China (NSFC)
- Contributing Org.:
- Univ. of Oklahoma, Norman, OK (United States)
- OSTI Identifier:
- 1337179
- Grant/Contract Number:
- SC0006351; 21105040; 20110211120010; AR11-003
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical Chemistry
- Additional Journal Information:
- Journal Volume: 86; Journal Issue: 24; Journal ID: ISSN 0003-2700
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Zhou, Lei, Lu, Joann Juan, Gu, Congying, and Liu, Shaorong. Binary Electroosmotic-Pump Nanoflow Gradient Generator for Miniaturized High-Performance Liquid Chromatography. United States: N. p., 2014.
Web. doi:10.1021/ac503223r.
Zhou, Lei, Lu, Joann Juan, Gu, Congying, & Liu, Shaorong. Binary Electroosmotic-Pump Nanoflow Gradient Generator for Miniaturized High-Performance Liquid Chromatography. United States. https://doi.org/10.1021/ac503223r
Zhou, Lei, Lu, Joann Juan, Gu, Congying, and Liu, Shaorong. Mon .
"Binary Electroosmotic-Pump Nanoflow Gradient Generator for Miniaturized High-Performance Liquid Chromatography". United States. https://doi.org/10.1021/ac503223r. https://www.osti.gov/servlets/purl/1337179.
@article{osti_1337179,
title = {Binary Electroosmotic-Pump Nanoflow Gradient Generator for Miniaturized High-Performance Liquid Chromatography},
author = {Zhou, Lei and Lu, Joann Juan and Gu, Congying and Liu, Shaorong},
abstractNote = {High-performance liquid chromatography (HPLC) plays an important role in biotechnology, and a majority of chromatographic separations use gradient elution. While gradient generators can be built in different formats, binary pumps or quaternary pumps are most frequently used for gradient generator constructions. We have recently developed a high-pressure electroosmotic pump (EOP); the pump can be manufactured at a cost of a few hundred dollars. However, it is challenging to use this pump to deliver a gradient eluent directly. In this study, we first improve the monolith preparation by applying a pressure to the monomer solution during polymerization. We assemble a binary EOP gradient generator and discuss the relationship between the gradient profile and voltage applied to the EOP. Here we demonstrate the feasibility of the binary EOP gradient generator for generating a smooth and reproducible nanoflow gradient. After integration of the gradient generator into a miniaturized HPLC system, we use the HPLC system for separating peptide mixtures from trypsin-digested proteins. Lastly, the performance comparison between the above miniaturized HPLC system and an Agilent 1200 HPLC system exhibits comparable efficiencies, resolutions, and peak capacities.},
doi = {10.1021/ac503223r},
journal = {Analytical Chemistry},
number = 24,
volume = 86,
place = {United States},
year = {Mon Nov 17 00:00:00 EST 2014},
month = {Mon Nov 17 00:00:00 EST 2014}
}
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Works referencing / citing this record:
Review: Electric field driven pumping in microfluidic device
journal, December 2017
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- ELECTROPHORESIS, Vol. 39, Issue 5-6