Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification
Abstract
The endocannabinoidsN-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups ofN-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example,N-palmitoylethanolamine (PEA),N-stearoylethanolamine (SEA), andN-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. In conclusion, the recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems.
- Authors:
-
- Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USA, Brookhaven National Laboratory, 50 Bell Avenue, Building 463, P.O. Box 5000, Upton, NY 11973-5000, USA
- Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USA
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1234050
- Alternate Identifier(s):
- OSTI ID: 1263472; OSTI ID: 1335430
- Report Number(s):
- BNL-112088-2016-JA
Journal ID: ISSN 2090-5904; PII: 2426398; 2426398
- Grant/Contract Number:
- FG02-05ER15647; SC00112704; FG02- 05ER15647
- Resource Type:
- Published Article
- Journal Name:
- Neural Plasticity
- Additional Journal Information:
- Journal Name: Neural Plasticity Journal Volume: 2016; Journal ID: ISSN 2090-5904
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Egypt
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Keereetaweep, Jantana, and Chapman, Kent D. Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification. Egypt: N. p., 2016.
Web. doi:10.1155/2016/2426398.
Keereetaweep, Jantana, & Chapman, Kent D. Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification. Egypt. https://doi.org/10.1155/2016/2426398
Keereetaweep, Jantana, and Chapman, Kent D. Fri .
"Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification". Egypt. https://doi.org/10.1155/2016/2426398.
@article{osti_1234050,
title = {Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification},
author = {Keereetaweep, Jantana and Chapman, Kent D.},
abstractNote = {The endocannabinoidsN-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups ofN-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example,N-palmitoylethanolamine (PEA),N-stearoylethanolamine (SEA), andN-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. In conclusion, the recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems.},
doi = {10.1155/2016/2426398},
journal = {Neural Plasticity},
number = ,
volume = 2016,
place = {Egypt},
year = {Fri Jan 01 00:00:00 EST 2016},
month = {Fri Jan 01 00:00:00 EST 2016}
}
https://doi.org/10.1155/2016/2426398
Web of Science
Works referenced in this record:
Isolation and structure of a brain constituent that binds to the cannabinoid receptor
journal, December 1992
- Devane, W.; Hanus, L.; Breuer, A.
- Science, Vol. 258, Issue 5090
Identification of an endogenous 2-monoglyceride, present in canine gut, that binds to cannabinoid receptors
journal, June 1995
- Mechoulam, Raphael; Ben-Shabat, Shimon; Hanus, Lumir
- Biochemical Pharmacology, Vol. 50, Issue 1
Changes in endocannabinoid and palmitoylethanolamide levels in eye tissues of patients with diabetic retinopathy and age-related macular degeneration
journal, December 2006
- Matias, I.; Wang, J. W.; Moriello, A. Schiano
- Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 75, Issue 6
Finding of endocannabinoids in human eye tissues: Implications for glaucoma
journal, May 2005
- Chen, June; Matias, Isabel; Dinh, Tim
- Biochemical and Biophysical Research Communications, Vol. 330, Issue 4
Quantification of endocannabinoids in postmortem brain of schizophrenic subjects
journal, August 2013
- Muguruza, Carolina; Lehtonen, Marko; Aaltonen, Niina
- Schizophrenia Research, Vol. 148, Issue 1-3
An introduction to the endocannabinoid system: from the early to the latest concepts
journal, February 2009
- De Petrocellis, Luciano; Di Marzo, Vincenzo
- Best Practice & Research Clinical Endocrinology & Metabolism, Vol. 23, Issue 1
The endocannabinoid system as a target for the treatment of cannabis dependence
journal, January 2009
- Clapper, Jason R.; Mangieri, Regina A.; Piomelli, Daniele
- Neuropharmacology, Vol. 56
Anandamide and 2-arachidonoylglycerol: Pharmacological Properties, Functional Features, and Emerging Specificities of the Two Major Endocannabinoids
journal, July 2012
- Luchicchi, Antonio; Pistis, Marco
- Molecular Neurobiology, Vol. 46, Issue 2
Molecular Characterization of a Phospholipase D Generating Anandamide and Its Congeners
journal, November 2003
- Okamoto, Yasuo; Morishita, Jun; Tsuboi, Kazuhito
- Journal of Biological Chemistry, Vol. 279, Issue 7
Anandamide Biosynthesis Catalyzed by the Phosphodiesterase GDE1 and Detection of Glycerophospho- N -acyl Ethanolamine Precursors in Mouse Brain
journal, January 2008
- Simon, Gabriel M.; Cravatt, Benjamin F.
- Journal of Biological Chemistry, Vol. 283, Issue 14
A biosynthetic pathway for anandamide
journal, August 2006
- Liu, J.; Wang, L.; Harvey-White, J.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 36
Multiple pathways involved in the biosynthesis of anandamide
journal, January 2008
- Liu, Jie; Wang, Lei; Harvey-White, Judith
- Neuropharmacology, Vol. 54, Issue 1
Biosynthesis of anandamide and N-palmitoylethanolamine by sequential actions of phospholipase A2 and lysophospholipase D
journal, June 2004
- Sun, Yong-Xin; Tsuboi, Kazuhito; Okamoto, Yasuo
- Biochemical Journal, Vol. 380, Issue 3
Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain
journal, November 2003
- Bisogno, Tiziana; Howell, Fiona; Williams, Gareth
- Journal of Cell Biology, Vol. 163, Issue 3
Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides
journal, November 1996
- Cravatt, Benjamin F.; Giang, Dan K.; Mayfield, Stephen P.
- Nature, Vol. 384, Issue 6604
Brain monoglyceride lipase participating in endocannabinoid inactivation
journal, July 2002
- Dinh, T. P.; Carpenter, D.; Leslie, F. M.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 16
Lipidomic analysis of endocannabinoid metabolism in biological samples
journal, September 2009
- Astarita, Giuseppe; Piomelli, Daniele
- Journal of Chromatography B, Vol. 877, Issue 26
The N-acylation-phosphodiesterase pathway and cell signalling
journal, May 1996
- Schmid, Harald H. O.; Schmid, Patricia C.; Natarajan, Viswanathan
- Chemistry and Physics of Lipids, Vol. 80, Issue 1-2
Endocannabinoids
journal, October 1998
- Mechoulam, Raphael; Fride, Ester; Di Marzo, Vincenzo
- European Journal of Pharmacology, Vol. 359, Issue 1
N-acylethanolamine accumulation in infarcted myocardium
journal, September 1979
- Epps, Dennis E.; Schmid, Patricia C.; Natarajan, V.
- Biochemical and Biophysical Research Communications, Vol. 90, Issue 2
Acute Stress Increases Circulating Anandamide and Other N-Acylethanolamines in Healthy Humans
journal, July 2012
- Dlugos, Andrea; Childs, Emma; Stuhr, Kara L.
- Neuropsychopharmacology, Vol. 37, Issue 11
Stress-induced generation of N-acylethanolamines in mouse epidermal JB6 P+ cells
journal, March 2000
- Berdyshev, Evgueni V.; Schmid, Patricia C.; Dong, Zigang
- Biochemical Journal, Vol. 346, Issue 2
Changes in brain levels of N-acylethanolamines and 2-arachidonoylglycerol in focal cerebral ischemia in mice
journal, December 2007
- Degn, Matilda; Lambertsen, Kate L.; Petersen, Gitte
- Journal of Neurochemistry, Vol. 103, Issue 5
The endocannabinoid system: a general view and latest additions
journal, March 2004
- Petrocellis, Luciano De; Cascio, Maria Grazia; Marzo, Vincenzo Di
- British Journal of Pharmacology, Vol. 141, Issue 5
Endocannabinoids and their oxygenation by cyclo-oxygenases, lipoxygenases and other oxygenases
journal, April 2015
- Urquhart, P.; Nicolaou, A.; Woodward, D. F.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1851, Issue 4
Metabolism of the Endocannabinoids, 2-Arachidonylglycerol and Anandamide, into Prostaglandin, Thromboxane, and Prostacyclin Glycerol Esters and Ethanolamides
journal, September 2002
- Kozak, Kevin R.; Crews, Brenda C.; Morrow, Jason D.
- Journal of Biological Chemistry, Vol. 277, Issue 47
Oxidative Metabolism of Endocannabinoids by COX-2
journal, February 2004
- Kozak, Kevin; Prusakiewicz, Jeffery; Marnett, Lawrence
- Current Pharmaceutical Design, Vol. 10, Issue 6
Lipid Signaling and Synaptic Plasticity
journal, October 2006
- Sang, Nan; Chen, Chu
- The Neuroscientist, Vol. 12, Issue 5
Synthesis of Prostaglandin E 2 Ethanolamide from Anandamide by Cyclooxygenase-2
journal, August 1997
- Yu, Ming; Ives, Danial; Ramesha, Chakkodabylu S.
- Journal of Biological Chemistry, Vol. 272, Issue 34
A six-month randomized clinical trial comparing the intraocular pressure-lowering efficacy of bimatoprost and latanoprost in patients with ocular hypertension or glaucoma
journal, January 2003
- Noecker, Robert S.; Dirks, Monte S.; Choplin, Neil T.
- American Journal of Ophthalmology, Vol. 135, Issue 1
A comparison of latanoprost, bimatoprost, and travoprost in patients with elevated intraocular pressure:
journal, May 2003
- Parrish, Richard K.; Palmberg, Paul; Sheu, Wang-Pui
- American Journal of Ophthalmology, Vol. 135, Issue 5
Human Platelets and Polymorphonuclear Leukocytes Synthesize Oxygenated Derivatives of Arachidonylethanolamide (Anandamide): Their Affinities for Cannabinoid Receptors and Pathways of Inactivation
journal, July 1998
- Edgemond, William S.; Hillard, Cecilia J.; Falck, J. R.
- Molecular Pharmacology, Vol. 54, Issue 1
A possible role of lipoxygenase in the activation of vanilloid receptors by anandamide in the guinea-pig bronchus
journal, September 2001
- Craib, Susan J.; Ellington, Heather C.; Pertwee, Roger G.
- British Journal of Pharmacology, Vol. 134, Issue 1
Lipoxygenase-catalyzed oxygenation of arachidonylethanolamide, a cannabinoid receptor agonist
journal, January 1995
- Ueda, Natsuo; Yamamoto, Kei; Yamamoto, Shozo
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 1254, Issue 2
Oxidation of the Endogenous Cannabinoid Arachidonoyl Ethanolamide by the Cytochrome P450 Monooxygenases: Physiological and Pharmacological Implications
journal, February 2010
- Snider, Natasha T.; Walker, Vyvyca J.; Hollenberg, Paul F.
- Pharmacological Reviews, Vol. 62, Issue 1
N-Acyl amino acids and N-acyl neurotransmitter conjugates: neuromodulators and probes for new drug targets: Acyl amino acid/neurotransmitter pharmacology
journal, May 2010
- Connor, Mark; Vaughan, Chris W.; Vandenberg, Robert J.
- British Journal of Pharmacology, Vol. 160, Issue 8
An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors
journal, June 2002
- Huang, S. M.; Bisogno, T.; Trevisani, M.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 12
Identification of a New Class of Molecules, the Arachidonyl Amino Acids, and Characterization of One Member That Inhibits Pain
journal, August 2001
- Huang, Susan M.; Bisogno, Tiziana; Petros, Timothy J.
- Journal of Biological Chemistry, Vol. 276, Issue 46
Analgesic actions of N -arachidonoyl-serotonin, a fatty acid amide hydrolase inhibitor with antagonistic activity at vanilloid TRPV1 receptors
journal, March 2007
- Maione, S.; Petrocellis, L.; Novellis, V.
- British Journal of Pharmacology, Vol. 150, Issue 6
Chemical characterization of a family of brain lipids that induce sleep
journal, June 1995
- Cravatt, B.; Prospero-Garcia, O.; Siuzdak, G.
- Science, Vol. 268, Issue 5216
Quantification of brain endocannabinoid levels: methods, interpretations and pitfalls: Quantification of brain eCBs
journal, March 2010
- Buczynski, Matthew W.; Parsons, Loren H.
- British Journal of Pharmacology, Vol. 160, Issue 3
Quantitative profiling of endocannabinoids and related compounds in rat brain using liquid chromatography–tandem electrospray ionization mass spectrometry
journal, January 2007
- Richardson, Denise; Ortori, Catharine A.; Chapman, Victoria
- Analytical Biochemistry, Vol. 360, Issue 2
Quantification of endocannabinoids in rat biological samples by GC/MS: Technical and theoretical considerations
journal, December 2006
- Hardison, Sarah; Weintraub, Susan T.; Giuffrida, Andrea
- Prostaglandins & Other Lipid Mediators, Vol. 81, Issue 3-4
Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry
journal, April 2011
- Lehtonen, Marko; Storvik, Markus; Malinen, Hanna
- Journal of Chromatography B, Vol. 879, Issue 11-12
Involvement of the Endocannabinoid System in Retinal Damage after High Intraocular Pressure–Induced Ischemia in Rats
journal, July 2007
- Nucci, Carlo; Gasperi, Valeria; Tartaglione, Rosanna
- Investigative Opthalmology & Visual Science, Vol. 48, Issue 7
Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase
journal, August 2010
- Kilaru, Aruna; Isaac, Giorgis; Tamura, Pamela
- Lipids, Vol. 45, Issue 9
Novel and sensitive method for the detection of anandamide by the use of its dansyl derivative
journal, March 2000
- Yagen, Boris; Burstein, Sumner
- Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 740, Issue 1
Quantification of endocannabinoids in biological systems by chromatography and mass spectrometry: A comprehensive review from an analytical and biological perspective
journal, November 2011
- Zoerner, Alexander A.; Gutzki, Frank-Mathias; Batkai, Sandor
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1811, Issue 11
Determination of anandamide and other fatty acyl ethanolamides in human serum by electrospray tandem mass spectrometry
journal, February 2007
- Schreiber, Daniela; Harlfinger, Stephanie; Nolden, Brit M.
- Analytical Biochemistry, Vol. 361, Issue 2
Ultra performance liquid chromatography tandem mass spectrometry method for the measurement of anandamide in human plasma
journal, September 2008
- Lam, Patricia M. W.; Marczylo, Timothy H.; El-Talatini, Mona
- Analytical Biochemistry, Vol. 380, Issue 2
Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters
journal, January 2008
- Wood, JodiAnne T.; Williams, John S.; Pandarinathan, Lakshmipathi
- Clinical Chemistry and Laboratory Medicine, Vol. 46, Issue 9
Endogenous ethanolamide analysis in human plasma using HPLC tandem MS with electrospray ionization
journal, July 2009
- Palandra, Joe; Prusakiewicz, Jeff; Ozer, Josef S.
- Journal of Chromatography B, Vol. 877, Issue 22
Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography–tandem mass spectrometry
journal, May 2009
- Balvers, Michiel G. J.; Verhoeckx, Kitty C. M.; Witkamp, Renger F.
- Journal of Chromatography B, Vol. 877, Issue 14-15
Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells
journal, September 2007
- Opitz, Christiane A.; Rimmerman, Neta; Zhang, Yanmin
- FEBS Letters, Vol. 581, Issue 25
A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
Quantification of Bioactive Acylethanolamides in Rat Plasma by Electrospray Mass Spectrometry
journal, April 2000
- Giuffrida, Andrea; Rodríguez de Fonseca, Fernando; Piomelli, Daniele
- Analytical Biochemistry, Vol. 280, Issue 1
Endogenous cannabinoids in post-mortem brains of Cloninger type 1 and 2 alcoholics
journal, April 2010
- Lehtonen, Marko; Storvik, Markus; Tupala, Erkki
- European Neuropsychopharmacology, Vol. 20, Issue 4
An optimized GC–MS method detects nanomolar amounts of anandamide in mouse brain
journal, February 2008
- Muccioli, Giulio G.; Stella, Nephi
- Analytical Biochemistry, Vol. 373, Issue 2
Targeted lipidomics approach for endogenous N-acyl amino acids in rat brain tissue
journal, September 2009
- Tan, Bo; William Yu, Y.; Francesca Monn, M.
- Journal of Chromatography B, Vol. 877, Issue 26
Differential Regulation of the Endocannabinoids Anandamide and 2-Arachidonylglycerol within the Limbic Forebrain by Dopamine Receptor Activity
journal, June 2003
- Patel, Sachin; Rademacher, David J.; Hillard, Cecilia J.
- Journal of Pharmacology and Experimental Therapeutics, Vol. 306, Issue 3
The postmortal accumulation of brain N -arachidonylethanolamine (anandamide) is dependent upon fatty acid amide hydrolase activity
journal, December 2004
- Patel, Sachin; Carrier, Erica J.; Ho, W-S. Vanessa
- Journal of Lipid Research, Vol. 46, Issue 2
Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines
journal, October 1995
- Fontana, A.; Di Marzo, V.; Cadas, H.
- Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 53, Issue 4
Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues
journal, February 2002
- Fezza, Filomena; Bisogno, Tiziana; Minassi, Alberto
- FEBS Letters, Vol. 513, Issue 2-3
Ether-linked analogue of 2-arachidonoylglycerol (noladin ether) was not detected in the brains of various mammalian species: Noladin ether was not detected in the brain
journal, May 2003
- Oka, Saori; Tsuchie, Akiko; Tokumura, Akira
- Journal of Neurochemistry, Vol. 85, Issue 6
Estimation of reference intervals of five endocannabinoids and endocannabinoid related compounds in human plasma by two dimensional-LC/MS/MS
journal, December 2011
- Fanelli, Flaminia; Di Lallo, Valentina D.; Belluomo, Ilaria
- Journal of Lipid Research, Vol. 53, Issue 3
High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-acylethanolamines
journal, November 2011
- Dumlao, Darren S.; Buczynski, Matthew W.; Norris, Paul C.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1811, Issue 11
A sensitive endocannabinoid assay. The simultaneous analysis of N-acylethanolamines and 2-monoacylglycerols
journal, February 2000
- Schmid, Patricia C.; Schwartz, Keith D.; Smith, Candice N.
- Chemistry and Physics of Lipids, Vol. 104, Issue 2
Simultaneous determination of endocannabinoids (arachidonylethanolamide and 2-arachidonylglycerol) and isoprostane (8-epiprostaglandin F2α) by gas chromatography–mass spectrometry-selected ion monitoring for medical samples
journal, July 2003
- Obata, T.; Sakurai, Y.; Kase, Y.
- Journal of Chromatography B, Vol. 792, Issue 1
N-acylethanolamines in seeds of selected legumes
journal, August 2005
- Venables, Barney J.; Waggoner, Cheryl A.; Chapman, Kent D.
- Phytochemistry, Vol. 66, Issue 16
Ethanolamide Oxylipins of Linolenic Acid Can Negatively Regulate Arabidopsis Seedling Development
journal, October 2013
- Keereetaweep, Jantana; Blancaflor, Elison B.; Hornung, Ellen
- The Plant Cell, Vol. 25, Issue 10
Astrocytes in Culture Produce Anandamide and Other Acylethanolamides
journal, March 2002
- Walter, Lisa; Franklin, Allyn; Witting, Anke
- Journal of Biological Chemistry, Vol. 277, Issue 23
Isotope dilution GC/MS determination of anandamide and other fatty acylethanolamides in rat blood plasma
journal, February 1998
- Giuffrida, Andrea; Piomelli, Daniele
- FEBS Letters, Vol. 422, Issue 3
Isotope Dilution Mass Spectrometric Measurements Indicate That Arachidonylethanolamide, the Proposed Endogenous Ligand of the Cannabinoid Receptor, Accumulates in Rat Brain Tissue Post Mortem but Is Contained at Low Levels in or Is Absent from Fresh Tissue
journal, July 1996
- Kempe, Kirsten; Hsu, Fong-Fu; Bohrer, Alan
- Journal of Biological Chemistry, Vol. 271, Issue 29
Targeted stable-isotope dilution GC–MS/MS analysis of the endocannabinoid anandamide and other fatty acid ethanol amides in human plasma
journal, September 2009
- Zoerner, Alexander A.; Gutzki, Frank M.; Suchy, Maria T.
- Journal of Chromatography B, Vol. 877, Issue 26
Gas chromatography-mass spectrometry analysis of endogenous cannabinoids in healthy and tumoral human brain and human cells in culture
journal, January 2001
- Maccarrone, Mauro; Attinà, Marina; Cartoni, Antonella
- Journal of Neurochemistry, Vol. 76, Issue 2
Analyses of Anandamide and Endocannabinoid-like Compounds Using Collision-induced Dissociation in Fast Atom Bombardment Ionization-Mass Spectrometry and Gas Chromatography/Chemical Ionization-Mass Spectrometry
journal, January 2003
- Kasai, Hiroko F.; Tsubuki, Masayoshi; Takahashi, Kazunori
- Analytical Sciences, Vol. 19, Issue 12
Endocannabinoid levels in rat limbic forebrain and hypothalamus in relation to fasting, feeding and satiation: stimulation of eating by 2-arachidonoyl glycerol
journal, June 2002
- Kirkham, Tim C.; Williams, Claire M.; Fezza, Filomena
- British Journal of Pharmacology, Vol. 136, Issue 4
Liquid chromatographic-atmospheric pressure chemical ionization mass spectrometric determination of anandamide and its analogs in rat brain and peripheral tissues
journal, March 1997
- Koga, Daisuke; Santa, Tomofumi; Fukushima, Takeshi
- Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 690, Issue 1-2
Quantification of anandamide, oleoylethanolamide and palmitoylethanolamide in rodent brain tissue using high performance liquid chromatography–electrospray mass spectroscopy
journal, August 2014
- Liput, Daniel J.; Tsakalozou, Eleftheria; Hammell, Dana C.
- Journal of Pharmaceutical Analysis, Vol. 4, Issue 4
Validation and application of an LC–MS/MS method for quantitation of three fatty acid ethanolamides as biomarkers for fatty acid hydrolase inhibition in human plasma
journal, June 2010
- Jian, Wenying; Edom, Richard; Weng, Naidong
- Journal of Chromatography B, Vol. 878, Issue 20
Simultaneous Measurement of Anandamide and 2-Arachidonoylglycerol by Polymyxin B-Selective Adsorption and Subsequent High-Performance Liquid Chromatography Analysis: Increase in Endogenous Cannabinoids in the Sera of Patients with Endotoxic Shock
journal, July 2001
- Wang, Yin; Liu, Yan; Ito, Yasuhiko
- Analytical Biochemistry, Vol. 294, Issue 1
Quantitative Method for the Profiling of the Endocannabinoid Metabolome by LC-Atmospheric Pressure Chemical Ionization-MS
journal, August 2007
- Williams, John; Wood, JodiAnne; Pandarinathan, Lakshmipathi
- Analytical Chemistry, Vol. 79, Issue 15
Simultaneous UPLC–MS/MS quantification of the endocannabinoids 2-arachidonoyl glycerol (2AG), 1-arachidonoyl glycerol (1AG), and anandamide in human plasma: Minimization of matrix-effects, 2AG/1AG isomerization and degradation by toluene solvent extraction
journal, February 2012
- Zoerner, Alexander A.; Batkai, Sandor; Suchy, Maria-Theresia
- Journal of Chromatography B, Vol. 883-884
Quantitative analysis of anandamide and related acylethanolamides in human seminal plasma by ultra performance liquid chromatography tandem mass spectrometry
journal, December 2010
- Amoako, Akwasi A.; Marczylo, Timothy H.; Lam, Patricia M. W.
- Journal of Chromatography B, Vol. 878, Issue 31
Analysis of endocannabinoids by Ag+ coordination tandem mass spectrometry
journal, March 2003
- Kingsley, Philip J.; Marnett, Lawrence J.
- Analytical Biochemistry, Vol. 314, Issue 1
Sex and hormonal cycle differences in rat brain levels of pain-related cannabimimetic lipid mediators
journal, August 2006
- Bradshaw, Heather B.; Rimmerman, Neta; Krey, Jocelyn F.
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 291, Issue 2
A second endogenous cannabinoid that modulates long-term potentiation
journal, August 1997
- Stella, Nephi; Schweitzer, Paul; Piomelli, Daniele
- Nature, Vol. 388, Issue 6644