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Title: Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes

Reactive oxygen species (ROS) are produced by and have the potential to be damaging to all aerobic organisms. In photosynthetic organisms, they are an unavoidable byproduct of electron transfer in both the chloroplast and mitochondrion. Here, in this paper, we employ the reference unicellular green alga Chlamydomonas reinhardtii to identify the effect of H 2O 2 on gene expression by monitoring the changes in the transcriptome in a time-course experiment. Comparison of transcriptomes from cells sampled immediately prior to the addition of H 2O 2 and 0.5 and 1 h subsequently revealed 1278 differentially abundant transcripts. Of those transcripts that increase in abundance, many encode proteins involved in ROS detoxification, protein degradation and stress responses, whereas among those that decrease are transcripts encoding proteins involved in photosynthesis and central carbon metabolism. In addition to these transcriptomic adjustments, we observe that addition of H 2O 2 is followed by an accumulation and oxidation of the total intracellular glutathione pool, and a decrease in photosynthetic O 2 output. Additionally, we analyze our transcriptomes in the context of changes in transcript abundance in response to singlet O 2 (O 2 *), and relate our H 2O 2-induced transcripts to a diurnal transcriptome, wheremore » we demonstrate enrichments of H 2O 2-induced transcripts early in the light phase, late in the light phase and 2 h prior to light. In conclusion, on this basis several genes that are highlighted in this work may be involved in previously undiscovered stress remediation pathways or acclimation responses.« less
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6]
  1. Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry; Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Department
  2. Sorbonne Universites, UPMC Univ., Paris (France); Laboratoire de Biologie Moleculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris (France); Instituto de Bioqui­mica Vegetal y Fotosi­ntesis, Consejo Superior de Investigaciones Cienti­ficas (CSIC)-Universidad de Sevilla, Seville (Spain)
  3. Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry
  4. Univ. of California, Los Angeles, CA (United States). Institute for Genomics and Proteomics
  5. Sorbonne Universites, UPMC Univ., Paris (France); Laboratoire de Biologie Moleculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris (France)
  6. Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry; Univ. of California, Los Angeles, CA (United States). Institute for Genomics and Proteomics
Publication Date:
Report Number(s):
BNL-111999-2016-JA
Journal ID: ISSN 0960-7412
Grant/Contract Number:
SC0012704; FD02-04ER15529
Type:
Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 84; Journal Issue: 5; Journal ID: ISSN 0960-7412
Publisher:
Society for Experimental Biology
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; algae; H2O2; RNA-seq; transcriptome; oxidative stress; stress responses; redox signaling; reactive oxygen species
OSTI Identifier:
1335416