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Title: Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy

Non-clustered δ-protocadherins are homophilic cell adhesion molecules essential for the development of the vertebrate nervous system, as several are closely linked to neurodevelopmental disorders. Mutations in protocadherin-19 ( PCDH19) result in a female-limited, infant-onset form of epilepsy (PCDH19-FE). Over 100 mutations in PCDH19 have been identified in patients with PCDH19-FE, about half of which are missense mutations in the adhesive extracellular domain. Neither the mechanism of homophilic adhesion by PCDH19, nor the biochemical effects of missense mutations are understood. Here we present a crystallographic structure of the minimal adhesive fragment of the zebrafish Pcdh19 extracellular domain. This structure reveals the adhesive interface for Pcdh19, which is broadly relevant to both non-clustered δ and clustered protocadherin subfamilies. Additionally, we show that several PCDH19-FE missense mutations localize to the adhesive interface and abolish Pcdh19 adhesion in in vitro assays, thus revealing the biochemical basis of their pathogenic effects during brain development.
Authors:
 [1] ; ORCiD logo [1] ; ORCiD logo [1]
  1. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
Grant/Contract Number:
R21MH09463; GUP 40277; FR-2015-65794
Type:
Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Research Org:
Office of Scientific and Technical Information, Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1332774
Alternate Identifier(s):
OSTI ID: 1332775; OSTI ID: 1375916

Cooper, Sharon R., Jontes, James D., and Sotomayor, Marcos. Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy. United States: N. p., Web. doi:10.7554/eLife.18529.
Cooper, Sharon R., Jontes, James D., & Sotomayor, Marcos. Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy. United States. doi:10.7554/eLife.18529.
Cooper, Sharon R., Jontes, James D., and Sotomayor, Marcos. 2016. "Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy". United States. doi:10.7554/eLife.18529.
@article{osti_1332774,
title = {Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy},
author = {Cooper, Sharon R. and Jontes, James D. and Sotomayor, Marcos},
abstractNote = {Non-clustered δ-protocadherins are homophilic cell adhesion molecules essential for the development of the vertebrate nervous system, as several are closely linked to neurodevelopmental disorders. Mutations in protocadherin-19 (PCDH19) result in a female-limited, infant-onset form of epilepsy (PCDH19-FE). Over 100 mutations in PCDH19 have been identified in patients with PCDH19-FE, about half of which are missense mutations in the adhesive extracellular domain. Neither the mechanism of homophilic adhesion by PCDH19, nor the biochemical effects of missense mutations are understood. Here we present a crystallographic structure of the minimal adhesive fragment of the zebrafish Pcdh19 extracellular domain. This structure reveals the adhesive interface for Pcdh19, which is broadly relevant to both non-clustered δ and clustered protocadherin subfamilies. Additionally, we show that several PCDH19-FE missense mutations localize to the adhesive interface and abolish Pcdh19 adhesion in in vitro assays, thus revealing the biochemical basis of their pathogenic effects during brain development.},
doi = {10.7554/eLife.18529},
journal = {eLife},
number = ,
volume = 5,
place = {United States},
year = {2016},
month = {10}
}

Works referenced in this record:

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journal, August 2009
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