A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin
Abstract
Summary Miscanthus spp. are promising lignocellulosic energy crops, but cell wall recalcitrance to deconstruction still hinders their widespread use as bioenergy and biomaterial feedstocks. Identification of cell wall characteristics desirable for biorefining applications is crucial for lignocellulosic biomass improvement. However, the task of scoring biomass quality is often complicated by the lack of a reference for a given feedstock. A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points. A comprehensive suite of 155 monoclonal antibodies was used to monitor changes in distribution, structure and extractability of noncellulosic cell wall matrix glycans. Glycan microarrays complemented with immunohistochemistry elucidated the nature of compositional variation, and in situ distribution of carbohydrate epitopes. Key observations demonstrated that there are crucial differences in miscanthus cell wall glycomes, which may impact biomass amenability to deconstruction. For the first time, variations in miscanthus cell wall glycan components were comprehensively characterized across different harvests, organs and genotypes, to generate a representative reference profile for miscanthus cell wall biomass. Ultimately, this portrait of the miscanthus cell wall will help to steer breeding and genetic engineering strategies for the development of superiormore »
- Authors:
-
- Institute of Biological Environmental and Rural Sciences Aberystwyth University Plas Gogerddan Aberystwyth Ceredigion SY23 3EE UK
- Complex Carbohydrate Research Center The University of Georgia 315 Riverbend Road Athens GA 30602 USA, US Department of Energy Bioenergy Science Center Oak Ridge National Laboratory Oak Ridge TN 37831 USA
- Biology Department University of Massachusetts Amherst MA 01003 USA
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Univ. of California, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1332376
- Alternate Identifier(s):
- OSTI ID: 1333022; OSTI ID: 1625915
- Grant/Contract Number:
- AC05-00OR22725; SC0006621
- Resource Type:
- Published Article
- Journal Name:
- New Phytologist
- Additional Journal Information:
- Journal Name: New Phytologist Journal Volume: 213 Journal Issue: 4; Journal ID: ISSN 0028-646X
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- Plant Sciences
Citation Formats
da Costa, Ricardo M. F., Pattathil, Sivakumar, Avci, Utku, Lee, Scott J., Hazen, Samuel P., Winters, Ana, Hahn, Michael G., and Bosch, Maurice. A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin. United Kingdom: N. p., 2016.
Web. doi:10.1111/nph.14306.
da Costa, Ricardo M. F., Pattathil, Sivakumar, Avci, Utku, Lee, Scott J., Hazen, Samuel P., Winters, Ana, Hahn, Michael G., & Bosch, Maurice. A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin. United Kingdom. https://doi.org/10.1111/nph.14306
da Costa, Ricardo M. F., Pattathil, Sivakumar, Avci, Utku, Lee, Scott J., Hazen, Samuel P., Winters, Ana, Hahn, Michael G., and Bosch, Maurice. Tue .
"A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin". United Kingdom. https://doi.org/10.1111/nph.14306.
@article{osti_1332376,
title = {A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin},
author = {da Costa, Ricardo M. F. and Pattathil, Sivakumar and Avci, Utku and Lee, Scott J. and Hazen, Samuel P. and Winters, Ana and Hahn, Michael G. and Bosch, Maurice},
abstractNote = {Summary Miscanthus spp. are promising lignocellulosic energy crops, but cell wall recalcitrance to deconstruction still hinders their widespread use as bioenergy and biomaterial feedstocks. Identification of cell wall characteristics desirable for biorefining applications is crucial for lignocellulosic biomass improvement. However, the task of scoring biomass quality is often complicated by the lack of a reference for a given feedstock. A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points. A comprehensive suite of 155 monoclonal antibodies was used to monitor changes in distribution, structure and extractability of noncellulosic cell wall matrix glycans. Glycan microarrays complemented with immunohistochemistry elucidated the nature of compositional variation, and in situ distribution of carbohydrate epitopes. Key observations demonstrated that there are crucial differences in miscanthus cell wall glycomes, which may impact biomass amenability to deconstruction. For the first time, variations in miscanthus cell wall glycan components were comprehensively characterized across different harvests, organs and genotypes, to generate a representative reference profile for miscanthus cell wall biomass. Ultimately, this portrait of the miscanthus cell wall will help to steer breeding and genetic engineering strategies for the development of superior energy crops.},
doi = {10.1111/nph.14306},
journal = {New Phytologist},
number = 4,
volume = 213,
place = {United Kingdom},
year = {Tue Nov 15 00:00:00 EST 2016},
month = {Tue Nov 15 00:00:00 EST 2016}
}
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Figures / Tables:
Table 1: Composition of miscanthus biomass expressed as percentage of cell wall material (CWM) DW (%CWM)
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