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Title: A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Abstract

Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem: Ustilago bromivora and Brachypodium sp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon. U. bromivora harbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver.

Authors:
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [2]; ORCiD logo [3];  [4];  [5];  [6];  [6];  [7];  [8]; ORCiD logo [9] more »;  [8];  [10]; ORCiD logo [11];  [12]; ORCiD logo [1] « less
  1. Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
  2. Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria
  3. Max Planck Institute for Plant Breeding Research, Cologne, Germany
  4. Vienna Biocenter Core Facilities GmbH, Vienna, Austria
  5. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
  6. DOE Joint Genome Institute, California, United States
  7. INRA UMR BIOGER, AgroParisTech, Université Paris-Saclay, Thiverval-Grignon, France
  8. Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
  9. Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany
  10. Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
  11. Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany, Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany
  12. Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1331828
Alternate Identifier(s):
OSTI ID: 1331830
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Name: eLife Journal Volume: 5; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English

Citation Formats

Rabe, Franziska, Bosch, Jason, Stirnberg, Alexandra, Guse, Tilo, Bauer, Lisa, Seitner, Denise, Rabanal, Fernando A., Czedik-Eysenberg, Angelika, Uhse, Simon, Bindics, Janos, Genenncher, Bianca, Navarrete, Fernando, Kellner, Ronny, Ekker, Heinz, Kumlehn, Jochen, Vogel, John P., Gordon, Sean P., Marcel, Thierry C., Münsterkötter, Martin, Walter, Mathias C., Sieber, Christian MK, Mannhaupt, Gertrud, Güldener, Ulrich, Kahmann, Regine, and Djamei, Armin. A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem. United States: N. p., 2016. Web. doi:10.7554/eLife.20522.
Rabe, Franziska, Bosch, Jason, Stirnberg, Alexandra, Guse, Tilo, Bauer, Lisa, Seitner, Denise, Rabanal, Fernando A., Czedik-Eysenberg, Angelika, Uhse, Simon, Bindics, Janos, Genenncher, Bianca, Navarrete, Fernando, Kellner, Ronny, Ekker, Heinz, Kumlehn, Jochen, Vogel, John P., Gordon, Sean P., Marcel, Thierry C., Münsterkötter, Martin, Walter, Mathias C., Sieber, Christian MK, Mannhaupt, Gertrud, Güldener, Ulrich, Kahmann, Regine, & Djamei, Armin. A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem. United States. doi:10.7554/eLife.20522.
Rabe, Franziska, Bosch, Jason, Stirnberg, Alexandra, Guse, Tilo, Bauer, Lisa, Seitner, Denise, Rabanal, Fernando A., Czedik-Eysenberg, Angelika, Uhse, Simon, Bindics, Janos, Genenncher, Bianca, Navarrete, Fernando, Kellner, Ronny, Ekker, Heinz, Kumlehn, Jochen, Vogel, John P., Gordon, Sean P., Marcel, Thierry C., Münsterkötter, Martin, Walter, Mathias C., Sieber, Christian MK, Mannhaupt, Gertrud, Güldener, Ulrich, Kahmann, Regine, and Djamei, Armin. Fri . "A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem". United States. doi:10.7554/eLife.20522.
@article{osti_1331828,
title = {A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem},
author = {Rabe, Franziska and Bosch, Jason and Stirnberg, Alexandra and Guse, Tilo and Bauer, Lisa and Seitner, Denise and Rabanal, Fernando A. and Czedik-Eysenberg, Angelika and Uhse, Simon and Bindics, Janos and Genenncher, Bianca and Navarrete, Fernando and Kellner, Ronny and Ekker, Heinz and Kumlehn, Jochen and Vogel, John P. and Gordon, Sean P. and Marcel, Thierry C. and Münsterkötter, Martin and Walter, Mathias C. and Sieber, Christian MK and Mannhaupt, Gertrud and Güldener, Ulrich and Kahmann, Regine and Djamei, Armin},
abstractNote = {Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem: Ustilago bromivora and Brachypodium sp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon. U. bromivora harbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver.},
doi = {10.7554/eLife.20522},
journal = {eLife},
number = ,
volume = 5,
place = {United States},
year = {2016},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.7554/eLife.20522

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