A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni
Abstract
A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. Lastly, we propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.
- Authors:
-
[3];
- Griffith Univ., Queensland (Australia)
- Univ. of Tennessee, Knoxville, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1330560
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; bacteria; pathogens
Citation Formats
Day, Christopher J., King, Rebecca M., Shewell, Lucy K., Tram, Greg, Najnin, Tahria, Hartley-Tassell, Lauren E., Wilson, Jennifer C., Fleetwood, Aaron D., Zhulin, Igor B., and Korolik, Victoria. A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni. United States: N. p., 2016.
Web. doi:10.1038/ncomms13206.
Day, Christopher J., King, Rebecca M., Shewell, Lucy K., Tram, Greg, Najnin, Tahria, Hartley-Tassell, Lauren E., Wilson, Jennifer C., Fleetwood, Aaron D., Zhulin, Igor B., & Korolik, Victoria. A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni. United States. https://doi.org/10.1038/ncomms13206
Day, Christopher J., King, Rebecca M., Shewell, Lucy K., Tram, Greg, Najnin, Tahria, Hartley-Tassell, Lauren E., Wilson, Jennifer C., Fleetwood, Aaron D., Zhulin, Igor B., and Korolik, Victoria. Thu .
"A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni". United States. https://doi.org/10.1038/ncomms13206. https://www.osti.gov/servlets/purl/1330560.
@article{osti_1330560,
title = {A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni},
author = {Day, Christopher J. and King, Rebecca M. and Shewell, Lucy K. and Tram, Greg and Najnin, Tahria and Hartley-Tassell, Lauren E. and Wilson, Jennifer C. and Fleetwood, Aaron D. and Zhulin, Igor B. and Korolik, Victoria},
abstractNote = {A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. Lastly, we propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.},
doi = {10.1038/ncomms13206},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {Thu Oct 20 00:00:00 EDT 2016},
month = {Thu Oct 20 00:00:00 EDT 2016}
}
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