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Title: Lipid droplets form from distinct regions of the cell in the fission yeast Schizosaccharomyces pombe

Abstract

Eukaryotic cells store cholesterol/sterol esters (SEs) and triacylglycerols (TAGs) in lipid droplets, which form from the contiguous endoplasmic reticulum (ER) network. However, it is not known if droplets preferentially form from certain regions of the ER over others. Here, we used fission yeast Schizosaccharomyces pombe cells where the nuclear and cortical/peripheral ER domains are distinguishable by light microscopy to show that SE-enriched lipid droplets form away from the nucleus at the cell tips, whereas TAG-enriched lipid droplets form around the nucleus. Sterols localize to the regions of the cells where droplets enriched in SEs are observed. TAG droplet formation around the nucleus appears to be a strong function of diacylglycerol (DAG) homeostasis with Cpt1p, which coverts DAG into phosphatidylcholine and phosphatidylethanolamine localized exclusively to the nuclear ER. Also, Dgk1p, which converts DAG into phosphatidic acid localized strongly to the nuclear ER over the cortical/peripheral ER. We also show that TAG more readily translocates from the ER to lipid droplets than do SEs. Lastly, the results augment the standard lipid droplet formation model, which has SEs and TAGs flowing into the same nascent lipid droplet regardless of its biogenesis point in the cell.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Work for Others (WFO)
OSTI Identifier:
1330554
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Traffic
Additional Journal Information:
Journal Volume: 17; Journal Issue: 6; Journal ID: ISSN 1398-9219
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Meyers, Alex, del Rio, Zuania P., Beaver, Rachael A., Morris, Ryan M., Weiskittel, Taylor M., Alshibli, Amany K., Mannik, Jaana, Morrell-Falvey, Jennifer, and Dalhaimer, Paul. Lipid droplets form from distinct regions of the cell in the fission yeast Schizosaccharomyces pombe. United States: N. p., 2016. Web. doi:10.1111/tra.12394.
Meyers, Alex, del Rio, Zuania P., Beaver, Rachael A., Morris, Ryan M., Weiskittel, Taylor M., Alshibli, Amany K., Mannik, Jaana, Morrell-Falvey, Jennifer, & Dalhaimer, Paul. Lipid droplets form from distinct regions of the cell in the fission yeast Schizosaccharomyces pombe. United States. doi:10.1111/tra.12394.
Meyers, Alex, del Rio, Zuania P., Beaver, Rachael A., Morris, Ryan M., Weiskittel, Taylor M., Alshibli, Amany K., Mannik, Jaana, Morrell-Falvey, Jennifer, and Dalhaimer, Paul. Fri . "Lipid droplets form from distinct regions of the cell in the fission yeast Schizosaccharomyces pombe". United States. doi:10.1111/tra.12394. https://www.osti.gov/servlets/purl/1330554.
@article{osti_1330554,
title = {Lipid droplets form from distinct regions of the cell in the fission yeast Schizosaccharomyces pombe},
author = {Meyers, Alex and del Rio, Zuania P. and Beaver, Rachael A. and Morris, Ryan M. and Weiskittel, Taylor M. and Alshibli, Amany K. and Mannik, Jaana and Morrell-Falvey, Jennifer and Dalhaimer, Paul},
abstractNote = {Eukaryotic cells store cholesterol/sterol esters (SEs) and triacylglycerols (TAGs) in lipid droplets, which form from the contiguous endoplasmic reticulum (ER) network. However, it is not known if droplets preferentially form from certain regions of the ER over others. Here, we used fission yeast Schizosaccharomyces pombe cells where the nuclear and cortical/peripheral ER domains are distinguishable by light microscopy to show that SE-enriched lipid droplets form away from the nucleus at the cell tips, whereas TAG-enriched lipid droplets form around the nucleus. Sterols localize to the regions of the cells where droplets enriched in SEs are observed. TAG droplet formation around the nucleus appears to be a strong function of diacylglycerol (DAG) homeostasis with Cpt1p, which coverts DAG into phosphatidylcholine and phosphatidylethanolamine localized exclusively to the nuclear ER. Also, Dgk1p, which converts DAG into phosphatidic acid localized strongly to the nuclear ER over the cortical/peripheral ER. We also show that TAG more readily translocates from the ER to lipid droplets than do SEs. Lastly, the results augment the standard lipid droplet formation model, which has SEs and TAGs flowing into the same nascent lipid droplet regardless of its biogenesis point in the cell.},
doi = {10.1111/tra.12394},
journal = {Traffic},
number = 6,
volume = 17,
place = {United States},
year = {2016},
month = {4}
}

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    Works referencing / citing this record:

    A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast
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    • Zhang, Xiao-Ran; He, Jia-Bei; Wang, Yi-Zheng
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    • G3: Genes|Genomes|Genetics, Vol. 8, Issue 6
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