skip to main content

DOE PAGESDOE PAGES

Title: α- tocopherol’s location in membranes is not affected by their composition

To this day, α-tocopherol's (aToc) role in humans is not well known. In previous studies, we have tried to connect aToc's biological function with its location in a-lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aToc's hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aToc's hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl- sn-glycero-3-phospho-L-serine (POPS), and Sphingomyelin bilayers, suggesting that aToc's location near the lipid water interface may be a universal property of vitamin E. Lastly, in light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.
Authors:
 [1] ;  [2] ;  [3] ;  [1]
  1. Brock Univ., St. Catharines, ON (Canada)
  2. Canadian Neutron Beam Centre, Chalk River, ON (Canada); Comenius Univ., Bratislava (Slovakia); Joint Institute for Nuclear Research, Moscow Region (Russia)
  3. Brock Univ., St. Catharines, ON (Canada); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Joint Institute for Neutron Sciences, Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 31; Journal Issue: 15; Journal ID: ISSN 0743-7463
Publisher:
American Chemical Society
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1330532

Marquardt, Drew, Kucerka, Norbert, Katsaras, John, and Harroun, Thad A. α- tocopherol’s location in membranes is not affected by their composition. United States: N. p., Web. doi:10.1021/la502605c.
Marquardt, Drew, Kucerka, Norbert, Katsaras, John, & Harroun, Thad A. α- tocopherol’s location in membranes is not affected by their composition. United States. doi:10.1021/la502605c.
Marquardt, Drew, Kucerka, Norbert, Katsaras, John, and Harroun, Thad A. 2014. "α- tocopherol’s location in membranes is not affected by their composition". United States. doi:10.1021/la502605c. https://www.osti.gov/servlets/purl/1330532.
@article{osti_1330532,
title = {α- tocopherol’s location in membranes is not affected by their composition},
author = {Marquardt, Drew and Kucerka, Norbert and Katsaras, John and Harroun, Thad A.},
abstractNote = {To this day, α-tocopherol's (aToc) role in humans is not well known. In previous studies, we have tried to connect aToc's biological function with its location in a-lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aToc's hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aToc's hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), and Sphingomyelin bilayers, suggesting that aToc's location near the lipid water interface may be a universal property of vitamin E. Lastly, in light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.},
doi = {10.1021/la502605c},
journal = {Langmuir},
number = 15,
volume = 31,
place = {United States},
year = {2014},
month = {10}
}