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Title: Calcium transport into the cells of the sea urchin larva in relation to spicule formation

Abstract

Here, we investigated the manner in which the sea urchin larva takes up calcium from its body cavity into the primary mesenchymal cells (PMCs) that are responsible for spicule formation. We used the membrane-impermeable fluorescent dye calcein and alexa-dextran, with or without a calcium channel inhibitor, and imaged the larvae in vivo with selective-plane illumination microscopy. Both fluorescent molecules are taken up from the body cavity into the PMCs and ectoderm cells, where the two labels are predominantly colocalized in particles, whereas the calcium-binding calcein label is mainly excluded from the endoderm and is concentrated in the spicules. The presence of vesicles and vacuoles inside the PMCs that have openings through the plasma membrane directly to the body cavity was documented using high-resolution cryo-focused ion beam-SEM serial imaging. Some of the vesicles and vacuoles are interconnected to form large networks. We suggest that these vacuolar networks are involved in direct sea water uptake. We conclude that the calcium pathway from the body cavity into cells involves nonspecific endocytosis of sea water with its calcium.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1329959
Alternate Identifier(s):
OSTI ID: 1464948
Grant/Contract Number:  
FG02-07ER15899; AD 364 2-1
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 45; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; SPIM imaging; endocytosis; in vivo imaging; biomineralization; cryo-FIB-SEM

Citation Formats

Vidavsky, Netta, Addadi, Sefi, Schertel, Andreas, Ben-Ezra, David, Shpigel, Muki, Addadi, Lia, and Weiner, Steve. Calcium transport into the cells of the sea urchin larva in relation to spicule formation. United States: N. p., 2016. Web. doi:10.1073/pnas.1612017113.
Vidavsky, Netta, Addadi, Sefi, Schertel, Andreas, Ben-Ezra, David, Shpigel, Muki, Addadi, Lia, & Weiner, Steve. Calcium transport into the cells of the sea urchin larva in relation to spicule formation. United States. doi:10.1073/pnas.1612017113.
Vidavsky, Netta, Addadi, Sefi, Schertel, Andreas, Ben-Ezra, David, Shpigel, Muki, Addadi, Lia, and Weiner, Steve. Mon . "Calcium transport into the cells of the sea urchin larva in relation to spicule formation". United States. doi:10.1073/pnas.1612017113.
@article{osti_1329959,
title = {Calcium transport into the cells of the sea urchin larva in relation to spicule formation},
author = {Vidavsky, Netta and Addadi, Sefi and Schertel, Andreas and Ben-Ezra, David and Shpigel, Muki and Addadi, Lia and Weiner, Steve},
abstractNote = {Here, we investigated the manner in which the sea urchin larva takes up calcium from its body cavity into the primary mesenchymal cells (PMCs) that are responsible for spicule formation. We used the membrane-impermeable fluorescent dye calcein and alexa-dextran, with or without a calcium channel inhibitor, and imaged the larvae in vivo with selective-plane illumination microscopy. Both fluorescent molecules are taken up from the body cavity into the PMCs and ectoderm cells, where the two labels are predominantly colocalized in particles, whereas the calcium-binding calcein label is mainly excluded from the endoderm and is concentrated in the spicules. The presence of vesicles and vacuoles inside the PMCs that have openings through the plasma membrane directly to the body cavity was documented using high-resolution cryo-focused ion beam-SEM serial imaging. Some of the vesicles and vacuoles are interconnected to form large networks. We suggest that these vacuolar networks are involved in direct sea water uptake. We conclude that the calcium pathway from the body cavity into cells involves nonspecific endocytosis of sea water with its calcium.},
doi = {10.1073/pnas.1612017113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 45,
volume = 113,
place = {United States},
year = {2016},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1073/pnas.1612017113

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Cited by: 3 works
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