skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b

Abstract

It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.

Authors:
 [1];  [1];  [2];  [1]
  1. Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering
  2. Iowa State Univ., Ames, IA (United States). Dept. of Biochemistry, Biophysics and Molecular Biology
Publication Date:
Research Org.:
Univ. of Michigan, Ann Arbor, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1329003
Grant/Contract Number:  
SC0006630
Resource Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Letters (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Letters (Online); Journal Volume: 363; Journal Issue: 13; Journal ID: ISSN 1574-6968
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; methanotrophy; methanol dehydrogenase; methane monooxygenase; rare earth elements; copper

Citation Formats

Gu, Wenyu, Farhan Ul Haque, Muhammad, DiSpirito, Alan A., and Semrau, Jeremy D. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b. United States: N. p., 2016. Web. doi:10.1093/femsle/fnw129.
Gu, Wenyu, Farhan Ul Haque, Muhammad, DiSpirito, Alan A., & Semrau, Jeremy D. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b. United States. doi:10.1093/femsle/fnw129.
Gu, Wenyu, Farhan Ul Haque, Muhammad, DiSpirito, Alan A., and Semrau, Jeremy D. Thu . "Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b". United States. doi:10.1093/femsle/fnw129. https://www.osti.gov/servlets/purl/1329003.
@article{osti_1329003,
title = {Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b},
author = {Gu, Wenyu and Farhan Ul Haque, Muhammad and DiSpirito, Alan A. and Semrau, Jeremy D.},
abstractNote = {It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.},
doi = {10.1093/femsle/fnw129},
journal = {FEMS Microbiology Letters (Online)},
number = 13,
volume = 363,
place = {United States},
year = {2016},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

XoxF Acts as the Predominant Methanol Dehydrogenase in the Type I Methanotroph Methylomicrobium buryatense
journal, February 2016

  • Chu, Frances; Lidstrom, Mary E.
  • Journal of Bacteriology, Vol. 198, Issue 8
  • DOI: 10.1128/JB.00959-15

Marker Exchange Mutagenesis of mxaF , Encoding the Large Subunit of the Mxa Methanol Dehydrogenase, in Methylosinus trichosporium OB3b
journal, December 2015

  • Farhan Ul Haque, Muhammad; Gu, Wenyu; DiSpirito, Alan A.
  • Applied and Environmental Microbiology, Vol. 82, Issue 5
  • DOI: 10.1128/AEM.03615-15

Cerium Regulates Expression of Alternative Methanol Dehydrogenases in Methylosinus trichosporium OB3b
journal, August 2015

  • Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan
  • Applied and Environmental Microbiology, Vol. 81, Issue 21
  • DOI: 10.1128/AEM.02542-15

Molecular structure of La3+-induced methanol dehydrogenase-like protein in Methylobacterium radiotolerans
journal, May 2011

  • Hibi, Yoshihisa; Asai, Kentaro; Arafuka, Hiroshi
  • Journal of Bioscience and Bioengineering, Vol. 111, Issue 5
  • DOI: 10.1016/j.jbiosc.2010.12.017

PQQ-dependent methanol dehydrogenases: rare-earth elements make a difference
journal, May 2014

  • Keltjens, Jan T.; Pol, Arjan; Reimann, Joachim
  • Applied Microbiology and Biotechnology, Vol. 98, Issue 14
  • DOI: 10.1007/s00253-014-5766-8

Microbially mediated cerium oxidation in sea water
journal, May 1990


A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1
journal, November 2012


Effect of nutrient uptake by plant roots on the fate of REEs in soil
journal, February 2006


Rare earth metals are essential for methanotrophic life in volcanic mudpots: Rare earth metals essential for methanotrophic life
journal, September 2013

  • Pol, Arjan; Barends, Thomas R. M.; Dietl, Andreas
  • Environmental Microbiology, Vol. 16, Issue 1
  • DOI: 10.1111/1462-2920.12249

Methanotrophs and copper
journal, July 2010


Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides
journal, September 1976


Lanthanide-Dependent Regulation of Methanol Oxidation Systems in Methylobacterium extorquens AM1 and Their Contribution to Methanol Growth
journal, February 2016

  • Vu, Huong N.; Subuyuj, Gabriel A.; Vijayakumar, Srividhya
  • Journal of Bacteriology, Vol. 198, Issue 8
  • DOI: 10.1128/JB.00937-15

Enrichment, Isolation and Some Properties of Methane-utilizing Bacteria
journal, May 1970

  • Whittenbury, R.; Phillips, K. C.; Wilkinson, J. F.
  • Journal of General Microbiology, Vol. 61, Issue 2
  • DOI: 10.1099/00221287-61-2-205

XoxF-Type Methanol Dehydrogenase from the Anaerobic Methanotroph “Candidatus Methylomirabilis oxyfera”
journal, December 2014

  • Wu, Ming L.; Wessels, Hans J. C. T.; Pol, Arjan
  • Applied and Environmental Microbiology, Vol. 81, Issue 4
  • DOI: 10.1128/AEM.03292-14

    Works referencing / citing this record:

    Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps
    journal, October 2019

    • Farhan Ul Haque, Muhammad; Crombie, Andrew T.; Murrell, J. Colin
    • Microbiome, Vol. 7, Issue 1
    • DOI: 10.1186/s40168-019-0741-3

    Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps
    journal, October 2019

    • Farhan Ul Haque, Muhammad; Crombie, Andrew T.; Murrell, J. Colin
    • Microbiome, Vol. 7, Issue 1
    • DOI: 10.1186/s40168-019-0741-3

    A Mutagenic Screen Identifies a TonB-Dependent Receptor Required for the Lanthanide Metal Switch in the Type I Methanotroph “ Methylotuvimicrobium buryatense ” 5GB1C
    journal, May 2019

    • Groom, Joseph D.; Ford, Stephanie M.; Pesesky, Mitchell W.
    • Journal of Bacteriology, Vol. 201, Issue 15
    • DOI: 10.1128/jb.00120-19

    Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability
    journal, October 2019

    • Wehrmann, Matthias; Berthelot, Charlotte; Billard, Patrick
    • Frontiers in Microbiology, Vol. 10
    • DOI: 10.3389/fmicb.2019.02494

    MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense
    journal, January 2016

    • Chu, Frances; Beck, David A. C.; Lidstrom, Mary E.
    • PeerJ, Vol. 4
    • DOI: 10.7717/peerj.2435