Negative cooperativity in the nitrogenase Fe protein electron delivery cycle
Abstract
Mo-dependent nitrogenase catalyzes the biological reduction of atmospheric dinitrogen (N2) to two ammonia (NH3) molecules, through the action of two component proteins, the MoFe protein and the Fe protein. The catalytic MoFe protein is a symmetric dimer of αβ units, each of which contains one active site FeMo-co (FeMo-co; [7Fe-9S-Mo-C-homocitrate]) and an electron-carrier P cluster. Each half of the nitrogenase ternary complex, in which one Fe protein with two bound ATP molecules has bound to each MoFe protein αβ unit, undergoes an electron transfer (ET) cycle with ET from a Fe protein [4Fe-4S] cluster into its αβ unit followed by the hydrolysis of the two ATP to two ADP and two Pi. The prevailing model holds that each αβ unit of the MoFe protein functions independently. We now report that the ET cycle exhibits negative cooperativity, with ET and ATP hydrolysis in one half of the ternary nitrogenase complex suppressing these processes in the other half. The observed ET, ATP hydrolysis, and Pi release behavior is captured in a global fit to a two-branch negative-cooperativity kinetic model. A possible mechanism for communication between the two halves of MoFe protein is suggested by normal mode analysis showing correlated and anti-correlated motionsmore »
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Biological Electron Transfer and Catalysis (BETCy)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1328477
- Alternate Identifier(s):
- OSTI ID: 1340770; OSTI ID: 1388830
- Report Number(s):
- PNNL-SA-111505
Journal ID: ISSN 0027-8424
- Grant/Contract Number:
- SC0012518; AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 40; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; ATP hydrolysis; conformational control; allosteric control; half-sites reactivity
Citation Formats
Danyal, Karamatullah, Shaw, Sudipta, Page, Taylor R., Duval, Simon, Horitani, Masaki, Marts, Amy R., Lukoyanov, Dmitriy, Dean, Dennis R., Raugei, Simone, Hoffman, Brian M., Seefeldt, Lance C., and Antony, Edwin. Negative cooperativity in the nitrogenase Fe protein electron delivery cycle. United States: N. p., 2016.
Web. doi:10.1073/pnas.1613089113.
Danyal, Karamatullah, Shaw, Sudipta, Page, Taylor R., Duval, Simon, Horitani, Masaki, Marts, Amy R., Lukoyanov, Dmitriy, Dean, Dennis R., Raugei, Simone, Hoffman, Brian M., Seefeldt, Lance C., & Antony, Edwin. Negative cooperativity in the nitrogenase Fe protein electron delivery cycle. United States. https://doi.org/10.1073/pnas.1613089113
Danyal, Karamatullah, Shaw, Sudipta, Page, Taylor R., Duval, Simon, Horitani, Masaki, Marts, Amy R., Lukoyanov, Dmitriy, Dean, Dennis R., Raugei, Simone, Hoffman, Brian M., Seefeldt, Lance C., and Antony, Edwin. Tue .
"Negative cooperativity in the nitrogenase Fe protein electron delivery cycle". United States. https://doi.org/10.1073/pnas.1613089113.
@article{osti_1328477,
title = {Negative cooperativity in the nitrogenase Fe protein electron delivery cycle},
author = {Danyal, Karamatullah and Shaw, Sudipta and Page, Taylor R. and Duval, Simon and Horitani, Masaki and Marts, Amy R. and Lukoyanov, Dmitriy and Dean, Dennis R. and Raugei, Simone and Hoffman, Brian M. and Seefeldt, Lance C. and Antony, Edwin},
abstractNote = {Mo-dependent nitrogenase catalyzes the biological reduction of atmospheric dinitrogen (N2) to two ammonia (NH3) molecules, through the action of two component proteins, the MoFe protein and the Fe protein. The catalytic MoFe protein is a symmetric dimer of αβ units, each of which contains one active site FeMo-co (FeMo-co; [7Fe-9S-Mo-C-homocitrate]) and an electron-carrier P cluster. Each half of the nitrogenase ternary complex, in which one Fe protein with two bound ATP molecules has bound to each MoFe protein αβ unit, undergoes an electron transfer (ET) cycle with ET from a Fe protein [4Fe-4S] cluster into its αβ unit followed by the hydrolysis of the two ATP to two ADP and two Pi. The prevailing model holds that each αβ unit of the MoFe protein functions independently. We now report that the ET cycle exhibits negative cooperativity, with ET and ATP hydrolysis in one half of the ternary nitrogenase complex suppressing these processes in the other half. The observed ET, ATP hydrolysis, and Pi release behavior is captured in a global fit to a two-branch negative-cooperativity kinetic model. A possible mechanism for communication between the two halves of MoFe protein is suggested by normal mode analysis showing correlated and anti-correlated motions between the two nitrogenase αβ halves. EPR spectra furthermore show small differences between those of resting-state and singly-reduced MoFe protein that can be attributed to an intra-complex allosteric perturbation of the resting-state FeMo-co in one αβ unit by reduction of FeMo-co in the other. This work is supported as a part of the Biological and Electron Transfer and Catalysis (EFRC) program, an Energy Frontiers Research Center funded by the US Department of Energy (DOE), Office of Science (DE-SC0012518) to LCS, by National Institutes of Health (NIH) grants HL 63203 and GM 111097to BMH, and R15GM110671 to EA, and by the Division of Chemical Sciences, Geosciences, and Bio-Sciences, DOE to SR. The protein production, ATP hydrolysis, and stopped flow electron transfer studies were supported by the EFRC program, phosphate release and pulse chase by the NIH, calculations by the DOE, and rapid freeze quench and data fitting by the NIH.},
doi = {10.1073/pnas.1613089113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 40,
volume = 113,
place = {United States},
year = {Tue Oct 04 00:00:00 EDT 2016},
month = {Tue Oct 04 00:00:00 EDT 2016}
}
https://doi.org/10.1073/pnas.1613089113
Web of Science
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