High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC
Abstract
The Staphylococcus aureus manganese transporter protein MntC is under investigation as a component of a prophylactic S.aureus vaccine. Passive immunization with monoclonal antibodies mAB 305-78-7 and mAB 305-101-8 produced using MntC was shown to significantly reduce S. aureus burden in an infant rat model of infection. Earlier interference mapping suggested that a total of 23 monoclonal antibodies generated against MntC could be subdivided into three interference groups, representing three independent immunogenic regions. In the current work binding epitopes for selected representatives of each of these interference groups (mAB 305-72-5 – group 1, mAB 305-78-7 – group 2, and mAB 305-101-8 – group 3) were mapped using Hydrogen-Deuterium Exchange Mass Spectrometry (DXMS). All of the identified epitopes are discontinuous, with binding surface formed by structural elements that are separated within the primary sequence of the protein but adjacent in the context of the three-dimensional structure. The approach was validated by co-crystallizing the Fab fragment of one of the antibodies (mAB 305-78-7) with MntC and solving the three-dimensional structure of the complex. X-ray results themselves and localization of the mAB 305-78-7 epitope were further validated using antibody binding experiments with MntC variants containing substitutions of key amino acid residues. These results providedmore »
- Authors:
-
- Pfizer Vaccine Research and Development, Pearl River, NY (United States)
- Pfizer Protein Engineering and Production, Cambridge, MA (United States)
- Univ. of California at San Diego, La Jolla, CA (United States)
- National Jewish Health, Denver, CO (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1328023
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS Pathogens
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 9; Journal ID: ISSN 1553-7374
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; deuterium; monoclonal antibodies; antibodies; epitope mapping; X-ray crystallography; enzyme-linked immunoassays; amino acid substitution; Staphylococcus aureus
Citation Formats
Gribenko, Alexey V., Parris, Kevin, Mosyak, Lidia, Li, Sheng, Handke, Luke, Hawkins, Julio C., Severina, Elena, Matsuka, Yury V., Anderson, Annaliesa S., and Zhang, Gongyi. High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC. United States: N. p., 2016.
Web. doi:10.1371/journal.ppat.1005908.
Gribenko, Alexey V., Parris, Kevin, Mosyak, Lidia, Li, Sheng, Handke, Luke, Hawkins, Julio C., Severina, Elena, Matsuka, Yury V., Anderson, Annaliesa S., & Zhang, Gongyi. High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC. United States. https://doi.org/10.1371/journal.ppat.1005908
Gribenko, Alexey V., Parris, Kevin, Mosyak, Lidia, Li, Sheng, Handke, Luke, Hawkins, Julio C., Severina, Elena, Matsuka, Yury V., Anderson, Annaliesa S., and Zhang, Gongyi. Fri .
"High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC". United States. https://doi.org/10.1371/journal.ppat.1005908. https://www.osti.gov/servlets/purl/1328023.
@article{osti_1328023,
title = {High Resolution Mapping of Bactericidal Monoclonal Antibody Binding Epitopes on Staphylococcus aureus Antigen MntC},
author = {Gribenko, Alexey V. and Parris, Kevin and Mosyak, Lidia and Li, Sheng and Handke, Luke and Hawkins, Julio C. and Severina, Elena and Matsuka, Yury V. and Anderson, Annaliesa S. and Zhang, Gongyi},
abstractNote = {The Staphylococcus aureus manganese transporter protein MntC is under investigation as a component of a prophylactic S.aureus vaccine. Passive immunization with monoclonal antibodies mAB 305-78-7 and mAB 305-101-8 produced using MntC was shown to significantly reduce S. aureus burden in an infant rat model of infection. Earlier interference mapping suggested that a total of 23 monoclonal antibodies generated against MntC could be subdivided into three interference groups, representing three independent immunogenic regions. In the current work binding epitopes for selected representatives of each of these interference groups (mAB 305-72-5 – group 1, mAB 305-78-7 – group 2, and mAB 305-101-8 – group 3) were mapped using Hydrogen-Deuterium Exchange Mass Spectrometry (DXMS). All of the identified epitopes are discontinuous, with binding surface formed by structural elements that are separated within the primary sequence of the protein but adjacent in the context of the three-dimensional structure. The approach was validated by co-crystallizing the Fab fragment of one of the antibodies (mAB 305-78-7) with MntC and solving the three-dimensional structure of the complex. X-ray results themselves and localization of the mAB 305-78-7 epitope were further validated using antibody binding experiments with MntC variants containing substitutions of key amino acid residues. These results provided insight into the antigenic properties of MntC and how these properties may play a role in protecting the hostagainst S. aureus infection by preventing the capture and transport of Mn2+, a key element that the pathogen uses to evade host immunity.},
doi = {10.1371/journal.ppat.1005908},
journal = {PLoS Pathogens},
number = 9,
volume = 12,
place = {United States},
year = {Fri Sep 30 00:00:00 EDT 2016},
month = {Fri Sep 30 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 21 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Protein hydrogen exchange at residue resolution by proteolytic fragmentation mass spectrometry analysis
journal, September 2013
- Kan, Z. -Y.; Walters, B. T.; Mayne, L.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigen
journal, February 2013
- Malito, Enrico; Faleri, Agnese; Lo Surdo, Paola
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 9
Design and Characterization of Epitope-Scaffold Immunogens That Present the Motavizumab Epitope from Respiratory Syncytial Virus
journal, June 2011
- McLellan, Jason S.; Correia, Bruno E.; Chen, Man
- Journal of Molecular Biology, Vol. 409, Issue 5
Evaluation of Approaches to Monitor Staphylococcus aureus Virulence Factor Expression during Human Disease
journal, February 2015
- Rozemeijer, Wouter; Fink, Pamela; Rojas, Eduardo
- PLOS ONE, Vol. 10, Issue 2
Early Hydrophobic Collapse of α1-Antitrypsin Facilitates Formation of a Metastable State: Insights from Oxidative Labeling and Mass Spectrometry
journal, November 2012
- Stocks, Bradley B.; Sarkar, Anindya; Wintrode, Patrick L.
- Journal of Molecular Biology, Vol. 423, Issue 5
Determination of the Mechanism of Action of Anti-FasL Antibody by Epitope Mapping and Homology Modeling
journal, August 2009
- Obungu, Victor H.; Gelfanova, Valentina; Rathnachalam, Radhakrishnan
- Biochemistry, Vol. 48, Issue 30
Protein structure change studied by hydrogen-deuterium exchange, functional labeling, and mass spectrometry
journal, May 2003
- Englander, J. J.; Del Mar, C.; Li, W.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 12
Differential Accessibility of a Rotavirus VP6 Epitope in Trimers Comprising Type I, II, or III Channels as Revealed by Binding of a Human Rotavirus VP6-Specific Antibody
journal, October 2013
- Aiyegbo, M. S.; Eli, I. M.; Spiller, B. W.
- Journal of Virology, Vol. 88, Issue 1
Epitope mapping of inhibitory antibodies targeting the C2 domain of coagulation factor VIII by hydrogen-deuterium exchange mass spectrometry
journal, December 2013
- Sevy, A. M.; Healey, J. F.; Deng, W.
- Journal of Thrombosis and Haemostasis, Vol. 11, Issue 12
Mapping epitopes on a protein antigen by the proteolysis of antigen-antibody complexes
journal, May 1986
- Jemmerson, R.; Paterson, Y.
- Science, Vol. 232, Issue 4753
Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus
journal, October 2013
- McLellan, J. S.; Chen, M.; Joyce, M. G.
- Science, Vol. 342, Issue 6158
IL-1β Epitope Mapping Using Site-Directed Mutagenesis and Hydrogen−Deuterium Exchange Mass Spectrometry Analysis
journal, August 2005
- Lu, Jirong; Witcher, Derrick R.; White, Melissa A.
- Biochemistry, Vol. 44, Issue 33
Conformational epitope mapping of Pru du 6, a major allergen from almond nut
journal, October 2013
- Willison, LeAnna N.; Zhang, Qian; Su, Mengna
- Molecular Immunology, Vol. 55, Issue 3-4
CED: a conformational epitope database
journal, January 2006
- Huang, Jian; Honda, Wataru
- BMC Immunology, Vol. 7, Issue 1
Staphylococcus aureus Manganese Transport Protein C Is a Highly Conserved Cell Surface Protein That Elicits Protective Immunity Against S. aureus and Staphylococcus epidermidis
journal, April 2012
- Anderson, Annaliesa S.; Scully, Ingrid L.; Timofeyeva, Yekaterina
- The Journal of Infectious Diseases, Vol. 205, Issue 11
Folding mechanism of the metastable serpin 1-antitrypsin
journal, March 2012
- Tsutsui, Y.; Dela Cruz, R.; Wintrode, P. L.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 12
Epitope Mapping of a 95 kDa Antigen in Complex with Antibody by Solution-Phase Amide Backbone Hydrogen/Deuterium Exchange Monitored by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
journal, September 2011
- Zhang, Qian; Willison, LeAnna N.; Tripathi, Pallavi
- Analytical Chemistry, Vol. 83, Issue 18
Epitope mapping of a monoclonal antibody against human thrombin by H/D-exchange mass spectrometry reveals selection of a diverse sequence in a highly conserved protein
journal, June 2002
- Baerga-Ortiz, Abel; Hughes, Carrie A.; Mandell, Jeffrey G.
- Protein Science, Vol. 11, Issue 6
Dynamics of cAPK Type IIβ Activation Revealed by Enhanced Amide H/2H Exchange Mass Spectrometry (DXMS)
journal, April 2003
- Hamuro, Yoshimoto; Zawadzki, Kerri M.; Kim, Jack S.
- Journal of Molecular Biology, Vol. 327, Issue 5
Differential hydrogen/deuterium exchange mass spectrometry analysis of protein–ligand interactions
journal, February 2011
- Chalmers, Michael J.; Busby, Scott A.; Pascal, Bruce D.
- Expert Review of Proteomics, Vol. 8, Issue 1
Primary structure effects on peptide group hydrogen exchange
journal, September 1993
- Bai, Yawen; Milne, John S.; Mayne, Leland
- Proteins: Structure, Function, and Genetics, Vol. 17, Issue 1
Molecular epitope identification by limited proteolysis of an immobilized antigen-antibody complex and mass spectrometric peptide mapping.
journal, December 1990
- Suckau, D.; Kohl, J.; Karwath, G.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 24
Structural Insights into Fibrinogen Dynamics Using Amide Hydrogen/Deuterium Exchange Mass Spectrometry
journal, July 2013
- Marsh, James J.; Guan, Henry S.; Li, Sheng
- Biochemistry, Vol. 52, Issue 32
MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake: Manganese uptake and oxidative stress in S. aureus
journal, May 2002
- Horsburgh, Malcolm J.; Wharton, Stephen J.; Cox, Alan G.
- Molecular Microbiology, Vol. 44, Issue 5
Core Sequence of PAPf39 Amyloid Fibrils and Mechanism of pH-Dependent Fibril Formation: The Role of Monomer Conformation
journal, December 2012
- French, Kinsley C.; Makhatadze, George I.
- Biochemistry, Vol. 51, Issue 51
Epitope mapping by amide hydrogen/deuterium exchange coupled with immobilization of antibody, on-line proteolysis, liquid chromatography and mass spectrometry
journal, March 2009
- Coales, Stephen J.; Tuske, Steven J.; Tomasso, Justine C.
- Rapid Communications in Mass Spectrometry, Vol. 23, Issue 5
Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
journal, October 2013
- Handke, Luke D.; Hawkins, Julio C.; Miller, Alita A.
- PLoS ONE, Vol. 8, Issue 10
Phage display: Concept, innovations, applications and future
journal, November 2010
- Pande, Jyoti; Szewczyk, Magdalena M.; Grover, Ashok K.
- Biotechnology Advances, Vol. 28, Issue 6
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Epitope mapping of human VWF A3 recognized by monoclonal antibody SZ-123 and SZ-125 using MALDI mass spectrometry
journal, August 2011
- Jiang, Miao; Zhao, Yiming; Shen, Fei
- International Journal of Hematology, Vol. 94, Issue 3
Emerging mass spectrometry-based approaches to probe protein–receptor interactions: Focus on overcoming physiological barriers
journal, July 2013
- Kaltashov, Igor A.; Bobst, Cedric E.; Nguyen, Son N.
- Advanced Drug Delivery Reviews, Vol. 65, Issue 8
Three-Dimensional Structure and Biophysical Characterization of Staphylococcus aureus Cell Surface Antigen–Manganese Transporter MntC
journal, September 2013
- Gribenko, Alexey; Mosyak, Lidia; Ghosh, Sharmistha
- Journal of Molecular Biology, Vol. 425, Issue 18
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications
journal, January 2014
- Wei, Hui; Mo, Jingjie; Tao, Li
- Drug Discovery Today, Vol. 19, Issue 1
Structural Analysis of Bacterial ABC Transporter Inhibition by an Antibody Fragment
journal, April 2015
- Ahuja, Shivani; Rougé, Lionel; Swem, Danielle L.
- Structure, Vol. 23, Issue 4
Structure and properties of -synuclein and other amyloids determined at the amino acid level
journal, October 2005
- Del Mar, C.; Greenbaum, E. A.; Mayne, L.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 43
Mapping of discontinuous conformational epitopes by amide hydrogen/deuterium exchange mass spectrometry and computational docking: EPITOPE MAPPING BY HDX-DOCK
journal, February 2012
- Pandit, Deepangi; Tuske, Steven J.; Coales, Stephen J.
- Journal of Molecular Recognition, Vol. 25, Issue 3
Two cross‐reactive monoclonal antibodies recognize overlapping epitopes on Neisseria meningitidis factor H binding protein but have different functional properties
journal, December 2013
- Faleri, Agnese; Santini, Laura; Brier, Sébastien
- The FASEB Journal, Vol. 28, Issue 4
[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997
- Otwinowski, Zbyszek; Minor, Wladek
- Macromolecular Crystallography Part A, 307-326
Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS
journal, January 2004
- Pantazatos, D.; Kim, J. S.; Klock, H. E.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 3
Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER
journal, March 2012
- Smart, Oliver S.; Womack, Thomas O.; Flensburg, Claus
- Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Mapping of discontinuous conformational epitopes by amide hydrogen/deuterium exchange mass spectrometry and computational docking: EPITOPE MAPPING BY HDX-DOCK
journal, February 2012
- Pandit, Deepangi; Tuske, Steven J.; Coales, Stephen J.
- Journal of Molecular Recognition, Vol. 25, Issue 3
What Is a B Cell Epitope
book, January 2019
- Van Regenmortel, Marc H. V.
- HIV/AIDS: Immunochemistry, Reductionism and Vaccine Design
Epitope mapping of human VWF A3 recognized by monoclonal antibody SZ-123 and SZ-125 using MALDI mass spectrometry
journal, August 2011
- Jiang, Miao; Zhao, Yiming; Shen, Fei
- International Journal of Hematology, Vol. 94, Issue 3
Emerging mass spectrometry-based approaches to probe protein–receptor interactions: Focus on overcoming physiological barriers
journal, July 2013
- Kaltashov, Igor A.; Bobst, Cedric E.; Nguyen, Son N.
- Advanced Drug Delivery Reviews, Vol. 65, Issue 8
Phage display: Concept, innovations, applications and future
journal, November 2010
- Pande, Jyoti; Szewczyk, Magdalena M.; Grover, Ashok K.
- Biotechnology Advances, Vol. 28, Issue 6
Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications
journal, January 2014
- Wei, Hui; Mo, Jingjie; Tao, Li
- Drug Discovery Today, Vol. 19, Issue 1
Applications of peptide arrays prepared by the SPOT-technology
journal, February 2001
- Reineke, Ulrich; Volkmer-Engert, Rudolf; Schneider-Mergener, Jens
- Current Opinion in Biotechnology, Vol. 12, Issue 1
Core Sequence of PAPf39 Amyloid Fibrils and Mechanism of pH-Dependent Fibril Formation: The Role of Monomer Conformation
journal, December 2012
- French, Kinsley C.; Makhatadze, George I.
- Biochemistry, Vol. 51, Issue 51
Structural Insights into Fibrinogen Dynamics Using Amide Hydrogen/Deuterium Exchange Mass Spectrometry
journal, July 2013
- Marsh, James J.; Guan, Henry S.; Li, Sheng
- Biochemistry, Vol. 52, Issue 32
Determination of the Mechanism of Action of Anti-FasL Antibody by Epitope Mapping and Homology Modeling
journal, August 2009
- Obungu, Victor H.; Gelfanova, Valentina; Rathnachalam, Radhakrishnan
- Biochemistry, Vol. 48, Issue 30
Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS
journal, January 2004
- Pantazatos, D.; Kim, J. S.; Klock, H. E.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 3
Structure and properties of -synuclein and other amyloids determined at the amino acid level
journal, October 2005
- Del Mar, C.; Greenbaum, E. A.; Mayne, L.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 43
Folding mechanism of the metastable serpin 1-antitrypsin
journal, March 2012
- Tsutsui, Y.; Dela Cruz, R.; Wintrode, P. L.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 12
Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigen
journal, February 2013
- Malito, Enrico; Faleri, Agnese; Lo Surdo, Paola
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 9
Protein structure change studied by hydrogen-deuterium exchange, functional labeling, and mass spectrometry
journal, May 2003
- Englander, J. J.; Del Mar, C.; Li, W.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 12
Protein hydrogen exchange at residue resolution by proteolytic fragmentation mass spectrometry analysis
journal, September 2013
- Kan, Z. -Y.; Walters, B. T.; Mayne, L.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
Molecular epitope identification by limited proteolysis of an immobilized antigen-antibody complex and mass spectrometric peptide mapping.
journal, December 1990
- Suckau, D.; Kohl, J.; Karwath, G.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 24
Staphylococcus aureus Manganese Transport Protein C Is a Highly Conserved Cell Surface Protein That Elicits Protective Immunity Against S. aureus and Staphylococcus epidermidis
journal, April 2012
- Anderson, Annaliesa S.; Scully, Ingrid L.; Timofeyeva, Yekaterina
- The Journal of Infectious Diseases, Vol. 205, Issue 11
Two cross‐reactive monoclonal antibodies recognize overlapping epitopes on Neisseria meningitidis factor H binding protein but have different functional properties
journal, December 2013
- Faleri, Agnese; Santini, Laura; Brier, Sébastien
- The FASEB Journal, Vol. 28, Issue 4
Epitope mapping of a monoclonal antibody against human thrombin by H/D-exchange mass spectrometry reveals selection of a diverse sequence in a highly conserved protein
journal, June 2002
- Baerga-Ortiz, Abel; Hughes, Carrie A.; Mandell, Jeffrey G.
- Protein Science, Vol. 11, Issue 6
Epitope mapping of inhibitory antibodies targeting the C2 domain of coagulation factor VIII by hydrogen-deuterium exchange mass spectrometry
journal, December 2013
- Sevy, A. M.; Healey, J. F.; Deng, W.
- Journal of Thrombosis and Haemostasis, Vol. 11, Issue 12
Evaluation of Approaches to Monitor Staphylococcus aureus Virulence Factor Expression during Human Disease
journal, February 2015
- Rozemeijer, Wouter; Fink, Pamela; Rojas, Eduardo
- PLOS ONE, Vol. 10, Issue 2
Differential hydrogen/deuterium exchange mass spectrometry analysis of protein–ligand interactions
journal, February 2011
- Chalmers, Michael J.; Busby, Scott A.; Pascal, Bruce D.
- Expert Review of Proteomics, Vol. 8, Issue 1
Works referencing / citing this record:
Anti-bacterial Monoclonal Antibodies
book, January 2017
- Nagy, Eszter; Nagy, Gábor; Power, Christine A.
- Recombinant Antibodies for Infectious Diseases
An overview of hydrogen deuterium exchange mass spectrometry (HDX-MS) in drug discovery
journal, August 2017
- Masson, Glenn R.; Jenkins, Meredith L.; Burke, John E.
- Expert Opinion on Drug Discovery, Vol. 12, Issue 10
The development of a staphylococcus aureus four antigen vaccine for use prior to elective orthopedic surgery
journal, September 2018
- Gurtman, A.; Begier, E.; Mohamed, N.
- Human Vaccines & Immunotherapeutics, Vol. 15, Issue 2
High-Definition Mapping of Four Spatially Distinct Neutralizing Epitope Clusters on RiVax, a Candidate Ricin Toxin Subunit Vaccine
journal, October 2017
- Toth, Ronald T.; Angalakurthi, Siva Krishna; Van Slyke, Greta
- Clinical and Vaccine Immunology, Vol. 24, Issue 12
Two Vaccines for Staphylococcus aureus Induce a B-Cell-Mediated Immune Response
journal, August 2018
- Dupont, Christopher D.; Scully, Ingrid L.; Zimnisky, Ross M.
- mSphere, Vol. 3, Issue 4
MntC-Dependent Manganese Transport Is Essential for Staphylococcus aureus Oxidative Stress Resistance and Virulence
journal, July 2018
- Handke, Luke D.; Gribenko, Alexey V.; Timofeyeva, Yekaterina
- mSphere, Vol. 3, Issue 4
Tracking Higher Order Protein Structure by Hydrogen-Deuterium Exchange Mass Spectrometry
journal, February 2019
- Benhaim, Mark; Lee, Kelly K.; Guttman, Miklos
- Protein & Peptide Letters, Vol. 26, Issue 1
A Collection of Single-Domain Antibodies that Crowd Ricin Toxin’s Active Site
journal, December 2018
- Angalakurthi, Siva; Vance, David; Rong, Yinghui
- Antibodies, Vol. 7, Issue 4
Persistence of Immune Responses Through 36 Months in Healthy Adults After Vaccination With a Novel Staphylococcus aureus 4-Antigen Vaccine (SA4Ag)
journal, December 2019
- Creech, C. Buddy; Frenck, Robert W.; Fiquet, Anne
- Open Forum Infectious Diseases, Vol. 7, Issue 1