Natural genetic variability reduces recalcitrance in poplar

Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev; Tuskan, Gerald A.; Wyman, Charles E.

doi:10.1186/s13068-016-0521-2

Title: Natural genetic variability reduces recalcitrance in poplar

Abstract

Here, lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.

Authors:: Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev; Tuskan, Gerald A.; Wyman, Charles E.

Publication Date:: Fri May 20 00:00:00 EDT 2016

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1618640

Alternate Identifier(s):: OSTI ID: 1327698

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 09 BIOMASS FUELS; rare poplar variants; liquid hot water pretreatment; high-throughput pretreatment and co-hydrolysis; Poplar ranks; lignin comparators

Citation Formats


                    Bhagia, Samarthya, Muchero, Wellington, Kumar, Rajeev, Tuskan, Gerald A., and Wyman, Charles E. Natural genetic variability reduces recalcitrance in poplar.  Netherlands: N. p., 2016. 
Web.  doi:10.1186/s13068-016-0521-2.

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                    Bhagia, Samarthya, Muchero, Wellington, Kumar, Rajeev, Tuskan, Gerald A., & Wyman, Charles E. Natural genetic variability reduces recalcitrance in poplar.  Netherlands.  https://doi.org/10.1186/s13068-016-0521-2

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                    Bhagia, Samarthya, Muchero, Wellington, Kumar, Rajeev, Tuskan, Gerald A., and Wyman, Charles E. Fri .  
"Natural genetic variability reduces recalcitrance in poplar".  Netherlands.  https://doi.org/10.1186/s13068-016-0521-2.

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@article{osti_1618640,

  title        = {Natural genetic variability reduces recalcitrance in poplar},

  author       = {Bhagia, Samarthya and Muchero, Wellington and Kumar, Rajeev and Tuskan, Gerald A. and Wyman, Charles E.},

  abstractNote = {Here, lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.},

  doi          = {10.1186/s13068-016-0521-2},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 9,

  place        = {Netherlands},

  year         = {Fri May 20 00:00:00 EDT 2016},

  month        = {Fri May 20 00:00:00 EDT 2016}

}

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Table 1: Hydrothermal pretreatment conditions applied to poplar variants

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Works referenced in this record:

Lignin Biosynthesis
journal, June 2003

Boerjan, Wout; Ralph, John; Baucher, Marie
Annual Review of Plant Biology, Vol. 54, Issue 1, p. 519-546
DOI: 10.1146/annurev.arplant.54.031902.134938

Lignin content in natural Populus variants affects sugar release
journal, March 2011

Studer, M. H.; DeMartini, J. D.; Davis, M. F.
Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
DOI: 10.1073/pnas.1009252108

Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

Chen, Fang; Dixon, Richard A.
Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
DOI: 10.1038/nbt1316

Geographical and environmental gradients shape phenotypic trait variation and genetic structure in Populus trichocarpa
journal, November 2013

McKown, Athena D.; Guy, Robert D.; Klápště, Jaroslav
New Phytologist, Vol. 201, Issue 4
DOI: 10.1111/nph.12601

Simultaneous Utilization of Cellobiose, Xylose, and Acetic Acid from Lignocellulosic Biomass for Biofuel Production by an Engineered Yeast Platform
journal, November 2014

Wei, Na; Oh, Eun Joong; Million, Gyver
ACS Synthetic Biology, Vol. 4, Issue 6
DOI: 10.1021/sb500364q

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005

Mosier, Nathan; Wyman, Charles; Dale, Bruce
Bioresource Technology, Vol. 96, Issue 6, p. 673-686
DOI: 10.1016/j.biortech.2004.06.025

Current Challenges in Commercially Producing Biofuels from Lignocellulosic Biomass
journal, January 2014

Balan, Venkatesh
ISRN Biotechnology, Vol. 2014
DOI: 10.1155/2014/463074

Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion]
journal, April 1987

Overend, R. P.; Chornet, E.; Gascoigne, J. A.
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 321, Issue 1561
DOI: 10.1098/rsta.1987.0029

Inhibition ofTrichoderma reesei cellulase by sugars and solvents
journal, July 1990

Holtzapple, Mark; Cognata, Mona; Shu, Yuancai
Biotechnology and Bioengineering, Vol. 36, Issue 3
DOI: 10.1002/bit.260360310

Population genomics of Populus trichocarpa identifies signatures of selection and adaptive trait associations
journal, August 2014

Evans, Luke M.; Slavov, Gancho T.; Rodgers-Melnick, Eli
Nature Genetics, Vol. 46, Issue 10
DOI: 10.1038/ng.3075

Biocommodity Engineering
journal, October 1999

Lynd, L. R.; Wyman, C. E.; Gerngross, T. U.
Biotechnology Progress, Vol. 15, Issue 5
DOI: 10.1021/bp990109e

High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus
journal, January 2015

Muchero, Wellington; Guo, Jianjun; DiFazio, Stephen P.
BMC Genomics, Vol. 16, Issue 1
DOI: 10.1186/s12864-015-1215-z

Challenges of the utilization of wood polymers: how can they be overcome?
journal, July 2011

Pu, Yunqiao; Kosa, Matyas; Kalluri, Udaya C.
Applied Microbiology and Biotechnology, Vol. 91, Issue 6
DOI: 10.1007/s00253-011-3350-z

Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose
journal, December 2007

Selig, M. J.; Viamajala, S.; Decker, S. R.
Biotechnology Progress, Vol. 23, Issue 6
DOI: 10.1021/bp0702018

Deactivation of cellulases by phenols
journal, January 2011

Ximenes, Eduardo; Kim, Youngmi; Mosier, Nathan
Enzyme and Microbial Technology, Vol. 48, Issue 1
DOI: 10.1016/j.enzmictec.2010.09.006

Advances and Developments in Strategies to Improve Strains of Saccharomyces cerevisiae and Processes to Obtain the Lignocellulosic Ethanol−A Review
journal, March 2012

Laluce, C.; Schenberg, A. C. G.; Gallardo, J. C. M.
Applied Biochemistry and Biotechnology, Vol. 166, Issue 8
DOI: 10.1007/s12010-012-9619-6

Measurement of protein using bicinchoninic acid
journal, October 1985

Smith, P. K.; Krohn, R. I.; Hermanson, G. T.
Analytical Biochemistry, Vol. 150, Issue 1
DOI: 10.1016/0003-2697(85)90442-7

Strong cellulase inhibition by Mannan polysaccharides in cellulose conversion to sugars: Strong Cellulase Inhibition by Heteromannans
journal, May 2014

Kumar, Rajeev; Wyman, Charles E.
Biotechnology and Bioengineering, Vol. 111, Issue 7
DOI: 10.1002/bit.25218

Poplar as a feedstock for biofuels: A review of compositional characteristics
journal, March 2010

Sannigrahi, Poulomi; Ragauskas, Arthur J.; Tuskan, Gerald A.
Biofuels, Bioproducts and Biorefining, Vol. 4, Issue 2
DOI: 10.1002/bbb.206

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011

Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E.
Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145
DOI: 10.1146/annurev-chembioeng-061010-114205

Changes in the enzymatic hydrolysis rate of Avicel cellulose with conversion
journal, January 2006

Yang, Bin; Willies, Deidre M.; Wyman, Charles E.
Biotechnology and Bioengineering, Vol. 94, Issue 6
DOI: 10.1002/bit.20942

Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release
journal, February 2010

Studer, Michael H.; DeMartini, Jaclyn D.; Brethauer, Simone
Biotechnology and Bioengineering, Vol. 105, Issue 2, p. 231-238
DOI: 10.1002/bit.22527

A review of cellulosic biofuel commercial-scale projects in the United States
journal, February 2013

Brown, Tristan R.; Brown, Robert C.
Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 3
DOI: 10.1002/bbb.1387

Gene Flow and Local Adaptation in Trees
journal, December 2007

Savolainen, Outi; Pyhäjärvi, Tanja; Knürr, Timo
Annual Review of Ecology, Evolution, and Systematics, Vol. 38, Issue 1
DOI: 10.1146/annurev.ecolsys.38.091206.095646

Works referencing / citing this record:

Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems
journal, November 2017

Thomas, Vanessa A.; Kothari, Ninad; Bhagia, Samarthya
Biotechnology for Biofuels, Vol. 10, Issue 1
DOI: 10.1186/s13068-017-0975-x

Bridging the gap between feedstock growers and users: the study of a coppice poplar-based biorefinery
journal, March 2018

Dou, Chang; Gustafson, Rick; Bura, Renata
Biotechnology for Biofuels, Vol. 11, Issue 1
DOI: 10.1186/s13068-018-1079-y

Sugar yield and composition of tubers from Jerusalem Artichoke ( Helianthus tuberosus ) irrigated with saline waters
journal, March 2018

Bhagia, Samarthya; Ferreira, Jorge F. S.; Kothari, Ninad
Biotechnology and Bioengineering, Vol. 115, Issue 6
DOI: 10.1002/bit.26582

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis
journal, April 2018

Wang, Jack P.; Matthews, Megan L.; Williams, Cranos M.
Nature Communications, Vol. 9, Issue 1
DOI: 10.1038/s41467-018-03863-z

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii
journal, August 2019

Straub, Christopher T.; Khatibi, Piyum A.; Wang, Jack P.
Nature Communications, Vol. 10, Issue 1
DOI: 10.1038/s41467-019-11376-6

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii
journal, June 2017

Zurawski, Jeffrey V.; Khatibi, Piyum A.; Akinosho, Hannah O.
Applied and Environmental Microbiology, Vol. 83, Issue 17
DOI: 10.1128/aem.00969-17

Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction
journal, October 2017

Conway, Jonathan M.; McKinley, Bennett S.; Seals, Nathaniel L.
Applied and Environmental Microbiology, Vol. 83, Issue 24
DOI: 10.1128/aem.01828-17

Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood
journal, February 2017

Johnson, Amanda M.; Kim, Hoon; Ralph, John
Biotechnology for Biofuels, Vol. 10, Issue 1
DOI: 10.1186/s13068-017-0734-z

Multiple levers for overcoming the recalcitrance of lignocellulosic biomass
journal, January 2019

Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad
Biotechnology for Biofuels, Vol. 12, Issue 1
DOI: 10.1186/s13068-019-1353-7

Emerging Technologies for the Production of Renewable Liquid Transport Fuels from Biomass Sources Enriched in Plant Cell Walls
journal, December 2016

Tan, Hwei-Ting; Corbin, Kendall R.; Fincher, Geoffrey B.
Frontiers in Plant Science, Vol. 7
DOI: 10.3389/fpls.2016.01854

The Economics of Rapid Multiplication of Hybrid Poplar Biomass Varieties
journal, May 2019

Stanton, Brian J.; Haiby, Kathy; Gantz, Carlos
Forests, Vol. 10, Issue 5
DOI: 10.3390/f10050446

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