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Title: Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wet disk milling

In this study, to reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150 °C for 60 min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. Lastly, after steam treatment at 150 °C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [3] ;  [4]
  1. National Inst. of Advanced Industrial Science and Technology (AIST), Hiroshima (Japan). Biomass Refinery Research Center
  2. National Inst. of Advanced Industrial Science and Technology (AIST), Hiroshima (Japan). Biomass Refinery Research Center; Kangwon National University (South Korea). Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Publication Date:
OSTI Identifier:
1327621
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Renewable Energy
Additional Journal Information:
Journal Volume: 76; Journal ID: ISSN 0960-1481
Publisher:
Elsevier
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES Hot-compressed water; Wet disk milling; Populus; Switchgrass; Biorefinery