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Title: Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation

Abstract

Nitrogen cycling is a microbial metabolic process essential for global ecological/agricultural balance. To investigate the link between the well-established ammonium and the alternative nitrate assimilation metabolic pathways, global isotope metabolomics was employed to examine three nitrate reducing bacteria using 15NO3 as a nitrogen source. In contrast to a control (Pseudomonas stutzeri RCH2), the results show that two of the isolates from Oak Ridge, Tennessee (Pseudomonas N2A2 and N2E2) utilize nitrate and ammonia for assimilation concurrently with differential labeling observed across multiple classes of metabolites including amino acids and nucleotides. The data reveal that the N2A2 and N2E2 strains conserve nitrogen-containing metabolites, indicating that the nitrate assimilation pathway is a conservation mechanism for the assimilation of nitrogen. Co-utilization of nitrate and ammonia is likely an adaption to manage higher levels of nitrite since the denitrification pathways utilized by the N2A2 and N2E2 strains from the Oak Ridge site are predisposed to the accumulation of the toxic nitrite. In conclusion, the use of global isotope metabolomics allowed for this adaptive strategy to be investigated, which would otherwise not have been possible to decipher.

Authors:
 [1];  [1];  [2];  [2];  [1];  [3];  [1];  [4];  [5];  [2];  [1]
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  1. The Scripps Research Institute, La Jolla, CA (United States)
  2. Univ. of Georgia, Athens, GA (United States)
  3. Univ. of Lausanne, Lausanne (Switzerland)
  4. Univ. of Missouri, Columbia, MO (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1326478
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
ACS Chemical Biology
Additional Journal Information:
Journal Volume: 11; Journal Issue: 6; Journal ID: ISSN 1554-8929
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENIGMA

Citation Formats

Kurczy, Michael E., Forsberg, Erica M., Thorgersen, Michael P., Poole, Farris L., Benton, H. Paul, Ivanisevic, Julijana, Tran, Minerva L., Wall, Judy D., Elias, Dwayne A., Adams, Michael W. W., and Siuzdak, Gary. Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation. United States: N. p., 2016. Web. doi:10.1021/acschembio.6b00082.
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Kurczy, Michael E., Forsberg, Erica M., Thorgersen, Michael P., Poole, Farris L., Benton, H. Paul, Ivanisevic, Julijana, Tran, Minerva L., Wall, Judy D., Elias, Dwayne A., Adams, Michael W. W., & Siuzdak, Gary. Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation. United States. https://doi.org/10.1021/acschembio.6b00082
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Kurczy, Michael E., Forsberg, Erica M., Thorgersen, Michael P., Poole, Farris L., Benton, H. Paul, Ivanisevic, Julijana, Tran, Minerva L., Wall, Judy D., Elias, Dwayne A., Adams, Michael W. W., and Siuzdak, Gary. Tue . "Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation". United States. https://doi.org/10.1021/acschembio.6b00082. https://www.osti.gov/servlets/purl/1326478.
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@article{osti_1326478,
title = {Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation},
author = {Kurczy, Michael E. and Forsberg, Erica M. and Thorgersen, Michael P. and Poole, Farris L. and Benton, H. Paul and Ivanisevic, Julijana and Tran, Minerva L. and Wall, Judy D. and Elias, Dwayne A. and Adams, Michael W. W. and Siuzdak, Gary},
abstractNote = {Nitrogen cycling is a microbial metabolic process essential for global ecological/agricultural balance. To investigate the link between the well-established ammonium and the alternative nitrate assimilation metabolic pathways, global isotope metabolomics was employed to examine three nitrate reducing bacteria using 15NO3 as a nitrogen source. In contrast to a control (Pseudomonas stutzeri RCH2), the results show that two of the isolates from Oak Ridge, Tennessee (Pseudomonas N2A2 and N2E2) utilize nitrate and ammonia for assimilation concurrently with differential labeling observed across multiple classes of metabolites including amino acids and nucleotides. The data reveal that the N2A2 and N2E2 strains conserve nitrogen-containing metabolites, indicating that the nitrate assimilation pathway is a conservation mechanism for the assimilation of nitrogen. Co-utilization of nitrate and ammonia is likely an adaption to manage higher levels of nitrite since the denitrification pathways utilized by the N2A2 and N2E2 strains from the Oak Ridge site are predisposed to the accumulation of the toxic nitrite. In conclusion, the use of global isotope metabolomics allowed for this adaptive strategy to be investigated, which would otherwise not have been possible to decipher.},
doi = {10.1021/acschembio.6b00082},
journal = {ACS Chemical Biology},
number = 6,
volume = 11,
place = {United States},
year = {Tue Apr 05 00:00:00 EDT 2016},
month = {Tue Apr 05 00:00:00 EDT 2016}
}
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https://doi.org/10.1021/acschembio.6b00082
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