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Title: A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers

Abstract

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. As a result, the method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.

Authors:
 [1];  [2];  [1];  [1];  [2];  [2];  [3];  [3];  [1]
  1. Bioenergy Science Center, Golden CO USA, National Bioenergy Center, National Renewable Energy Laboratory, Golden CO USA
  2. Great Lakes BioEnergy Research Center, Michigan State University, East Lansing MI USA
  3. Wisconsin Bioenergy Initiative, University of Wisconsin, Madison WI USA
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1324472
Alternate Identifier(s):
OSTI ID: 1328999; OSTI ID: 1345672
Report Number(s):
NREL/JA-5100-65297
Journal ID: ISSN 1860-6768
Grant/Contract Number:  
DOE Office of Science BER DE-FC02-07ER64494; No. DE-AC36-08-GO28308; AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Name: Biotechnology Journal Journal Volume: 11 Journal Issue: 10; Journal ID: ISSN 1860-6768
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Germany
Language:
English
Subject:
09 BIOMASS FUELS; cell wall structure; lignin; S/G ratio; thioacidolysis

Citation Formats

Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, and Davis, Mark F. A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers. Germany: N. p., 2016. Web. doi:10.1002/biot.201600266.
Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, & Davis, Mark F. A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers. Germany. doi:https://doi.org/10.1002/biot.201600266
Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, and Davis, Mark F. Wed . "A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers". Germany. doi:https://doi.org/10.1002/biot.201600266.
@article{osti_1324472,
title = {A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers},
author = {Harman-Ware, Anne E. and Foster, Cliff and Happs, Renee M. and Doeppke, Crissa and Meunier, Kristoffer and Gehan, Jackson and Yue, Fengxia and Lu, Fachuang and Davis, Mark F.},
abstractNote = {Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. As a result, the method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.},
doi = {10.1002/biot.201600266},
journal = {Biotechnology Journal},
number = 10,
volume = 11,
place = {Germany},
year = {2016},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: https://doi.org/10.1002/biot.201600266

Citation Metrics:
Cited by: 4 works
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