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Title: A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. As a result, the method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1]
  1. Bioenergy Science Center, Golden, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
  3. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-65297
Journal ID: ISSN 1860-6768
Grant/Contract Number:
AC36-08GO28308; DOE Office of Science BER DE-FC02-07ER64494; No. DE-AC36-08-GO28308
Type:
Published Article
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Volume: 11; Journal Issue: 10; Journal ID: ISSN 1860-6768
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; cell wall structure; lignin; S/G ratio; thioacidolysis
OSTI Identifier:
1324472
Alternate Identifier(s):
OSTI ID: 1328999; OSTI ID: 1345672

Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, and Davis, Mark F.. A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers. United States: N. p., Web. doi:10.1002/biot.201600266.
Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, & Davis, Mark F.. A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers. United States. doi:10.1002/biot.201600266.
Harman-Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, and Davis, Mark F.. 2016. "A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers". United States. doi:10.1002/biot.201600266.
@article{osti_1324472,
title = {A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers},
author = {Harman-Ware, Anne E. and Foster, Cliff and Happs, Renee M. and Doeppke, Crissa and Meunier, Kristoffer and Gehan, Jackson and Yue, Fengxia and Lu, Fachuang and Davis, Mark F.},
abstractNote = {Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. As a result, the method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.},
doi = {10.1002/biot.201600266},
journal = {Biotechnology Journal},
number = 10,
volume = 11,
place = {United States},
year = {2016},
month = {9}
}

Works referenced in this record:

Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition
journal, June 2011