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Title: Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice

Abstract

Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. In conclusion, CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.

Authors:
 [1];  [2];  [1];  [1];  [3];  [1];  [1];  [1];  [2];  [3];  [1];  [4];  [2];  [3];  [1];  [5];  [5]
  1. Purdue Univ., West Lafayette, IN (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
  4. Northeastern Univ., Boston, MA (United States)
  5. Purdue Univ., West Lafayette, IN (United States); Univ. of Maryland, College Park, MD (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1324324
Alternate Identifier(s):
OSTI ID: 1324325; OSTI ID: 1325506
Report Number(s):
NREL/JA-2700-66087
Journal ID: ISSN 1467-7644
Grant/Contract Number:  
AC36-08GO28308; SC0000997; AC02-06CH11357; FG02-06ER1580
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 10; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANAYLYTICAL CHEMISTRY; biofuels; cell walls; secretion; iron-binding peptide; carbohydrate-binding module (CBM); iron concentration

Citation Formats

Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., and Peer, Wendy A. Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice. United States: N. p., 2016. Web. doi:10.1111/pbi.12557.
Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., & Peer, Wendy A. Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice. United States. doi:10.1111/pbi.12557.
Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., and Peer, Wendy A. Thu . "Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice". United States. doi:10.1111/pbi.12557.
@article{osti_1324324,
title = {Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice},
author = {Yang, Haibing and Wei, Hui and Ma, Guojie and Antunes, Mauricio S. and Vogt, Stefan and Cox, Joseph and Zhang, Xiao and Liu, Xiping and Bu, Lintao and Gleber, S. Charlotte and Carpita, Nicholas C. and Makowski, Lee and Himmel, Michael E. and Tucker, Melvin P. and McCann, Maureen C. and Murphy, Angus S. and Peer, Wendy A.},
abstractNote = {Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. In conclusion, CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.},
doi = {10.1111/pbi.12557},
journal = {Plant Biotechnology Journal},
number = 10,
volume = 14,
place = {United States},
year = {2016},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.12557

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Cited by: 4 works
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Works referenced in this record:

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