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Title: Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species

Although it is well known that low nitrogen stimulates lipid accumulation, especially for algae and some oleaginous yeast, few studies have been conducted in fungal species, especially on the impact of different nitrogen deficiency strategies. In this study, we use two promising consolidated bioprocessing (CBP) candidates to examine the impact of two nitrogen deficiency strategies on lipid production, which are the extensively investigated oleaginous yeast Yarrowia lipolytica, and the commercial cellulase producer Trichoderma reesei. We first utilized bioinformatics approaches to reconstruct the fatty acid metabolic pathway and demonstrated the presence of a triacylglycerol (TAG) biosynthesis pathway in Trichoderma reesei. We then examined the lipid production of Trichoderma reesei and Y. lipomyces in different media using two nitrogen deficiency strategies of nitrogen natural repletion and nitrogen depletion through centrifugation. Our results demonstrated that nitrogen depletion was better than nitrogen repletion with about 30% lipid increase for Trichoderma reesei and Y. lipomyces, and could be an option to improve lipid production in both oleaginous yeast and filamentous fungal species. The resulting distinctive lipid composition profiles indicated that the impacts of nitrogen depletion on yeast were different from those for fungal species. Under three types of C/N ratio conditions, C16 and C18 fattymore » acids were the predominant forms of lipids for both Trichoderma reesei and Y. lipolytica. In addition, while the overall fatty acid methyl ester (FAME) profiles of Trichoderma reesei were similar, the overall FAME profiles of Y. lipolytica observed a shift. The fatty acid metabolic pathway reconstructed in this work supports previous reports of lipid production in T. reesei, and provides a pathway for future omics studies and metabolic engineering efforts. Further investigation to identify the genetic targets responsible for the effect of nitrogen depletion on lipid production improvement will facilitate strain engineering to boost lipid production under more optimal conditions for productivity than those required for nitrogen depletion.« less
Authors:
ORCiD logo [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [2] ;  [2]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States); Hubei Univ., Wuhan (China)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-67021
Journal ID: ISSN 1996-1073; ENERGA
Grant/Contract Number:
AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Energies (Basel)
Additional Journal Information:
Journal Name: Energies (Basel); Journal Volume: 9; Journal Issue: 9; Journal ID: ISSN 1996-1073
Publisher:
MDPI AG
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; advanced biofuel; lipids; nitrogen depletion; nitrogen repletion; Trichoderma reesei; Yarrowia lipolytica
OSTI Identifier:
1319280

Yang, Shihui, Wang, Wei, Wei, Hui, Van Wychen, Stefanie, Pienkos, Philip T., Zhang, Min, and Himmel, Michael E.. Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species. United States: N. p., Web. doi:10.3390/en9090685.
Yang, Shihui, Wang, Wei, Wei, Hui, Van Wychen, Stefanie, Pienkos, Philip T., Zhang, Min, & Himmel, Michael E.. Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species. United States. doi:10.3390/en9090685.
Yang, Shihui, Wang, Wei, Wei, Hui, Van Wychen, Stefanie, Pienkos, Philip T., Zhang, Min, and Himmel, Michael E.. 2016. "Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species". United States. doi:10.3390/en9090685. https://www.osti.gov/servlets/purl/1319280.
@article{osti_1319280,
title = {Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species},
author = {Yang, Shihui and Wang, Wei and Wei, Hui and Van Wychen, Stefanie and Pienkos, Philip T. and Zhang, Min and Himmel, Michael E.},
abstractNote = {Although it is well known that low nitrogen stimulates lipid accumulation, especially for algae and some oleaginous yeast, few studies have been conducted in fungal species, especially on the impact of different nitrogen deficiency strategies. In this study, we use two promising consolidated bioprocessing (CBP) candidates to examine the impact of two nitrogen deficiency strategies on lipid production, which are the extensively investigated oleaginous yeast Yarrowia lipolytica, and the commercial cellulase producer Trichoderma reesei. We first utilized bioinformatics approaches to reconstruct the fatty acid metabolic pathway and demonstrated the presence of a triacylglycerol (TAG) biosynthesis pathway in Trichoderma reesei. We then examined the lipid production of Trichoderma reesei and Y. lipomyces in different media using two nitrogen deficiency strategies of nitrogen natural repletion and nitrogen depletion through centrifugation. Our results demonstrated that nitrogen depletion was better than nitrogen repletion with about 30% lipid increase for Trichoderma reesei and Y. lipomyces, and could be an option to improve lipid production in both oleaginous yeast and filamentous fungal species. The resulting distinctive lipid composition profiles indicated that the impacts of nitrogen depletion on yeast were different from those for fungal species. Under three types of C/N ratio conditions, C16 and C18 fatty acids were the predominant forms of lipids for both Trichoderma reesei and Y. lipolytica. In addition, while the overall fatty acid methyl ester (FAME) profiles of Trichoderma reesei were similar, the overall FAME profiles of Y. lipolytica observed a shift. The fatty acid metabolic pathway reconstructed in this work supports previous reports of lipid production in T. reesei, and provides a pathway for future omics studies and metabolic engineering efforts. Further investigation to identify the genetic targets responsible for the effect of nitrogen depletion on lipid production improvement will facilitate strain engineering to boost lipid production under more optimal conditions for productivity than those required for nitrogen depletion.},
doi = {10.3390/en9090685},
journal = {Energies (Basel)},
number = 9,
volume = 9,
place = {United States},
year = {2016},
month = {8}
}

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