Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis
Abstract
Among the R2R3 MYB transcription factors that involve in the regulation of secondary cell wall formation in Arabidopsis, MYB46 alone is sufficient to induce the entire secondary cell wall biosynthesis program. PtrMYB021, the poplar homolog of MYB46, has been reported to regulate secondary cell wall formation when expressed in Arabidopsis. We report here that spatially and temporally restricted expression of PtrMYB021 is critical for its function in regulating secondary cell wall formation. By using quantitative RT-PCR, we found that PtrMYB021 was expressed primarily in xylem tissues. When expressed in Arabidopsis under the control of PtrCesA8, but not the 35S promoter, PtrMYB021 increased secondary cell wall thickness, which is likely caused by increased lignification as well as changes in cell wall carbohydrate composition. Consistent with this, elevated expression of lignin and cellulose biosynthetic genes were observed in the transgenic plants. Finally, when expressed in Arabidopsis protoplasts as fusion proteins to the Gal4 DNA binding domain, PtrMYB021 activated the reporter gene Gal4-GUS. In summary, our results suggest that PtrMYB021 is a transcriptional activator, and spatially and temporally restricted expression of PtrMYB021 in Arabidopsis regulates secondary cell wall formation by activating a subset of secondary cell wall biosynthesis genes.
- Authors:
-
- Northeast Normal Univ. Changchun (China). Key Lab. of Molecular Epigenetics of MOE
- Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Botany
- Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Wood Science
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; Northeast Normal University; Genome British Columbia Applied Genomics Innovation Program
- OSTI Identifier:
- 1319199
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Plant Biology
- Additional Journal Information:
- Journal Volume: 59; Journal Issue: 1; Journal ID: ISSN 1226-9239
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Arabidopsis thaliana; Populus trichocarpa; PtrMYB021; R2R3 MYB; Secondary cell wall biosynthesis; Transcription factor
Citation Formats
Wang, Wei, Li, Eryang, Porth, Ilga, Chen, Jin-Gui, Mansfield, Shawn D., Douglas, Carl J., and Wang, Shucai. Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis. United States: N. p., 2016.
Web. doi:10.1007/s12374-016-0438-0.
Wang, Wei, Li, Eryang, Porth, Ilga, Chen, Jin-Gui, Mansfield, Shawn D., Douglas, Carl J., & Wang, Shucai. Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis. United States. https://doi.org/10.1007/s12374-016-0438-0
Wang, Wei, Li, Eryang, Porth, Ilga, Chen, Jin-Gui, Mansfield, Shawn D., Douglas, Carl J., and Wang, Shucai. Tue .
"Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis". United States. https://doi.org/10.1007/s12374-016-0438-0. https://www.osti.gov/servlets/purl/1319199.
@article{osti_1319199,
title = {Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis},
author = {Wang, Wei and Li, Eryang and Porth, Ilga and Chen, Jin-Gui and Mansfield, Shawn D. and Douglas, Carl J. and Wang, Shucai},
abstractNote = {Among the R2R3 MYB transcription factors that involve in the regulation of secondary cell wall formation in Arabidopsis, MYB46 alone is sufficient to induce the entire secondary cell wall biosynthesis program. PtrMYB021, the poplar homolog of MYB46, has been reported to regulate secondary cell wall formation when expressed in Arabidopsis. We report here that spatially and temporally restricted expression of PtrMYB021 is critical for its function in regulating secondary cell wall formation. By using quantitative RT-PCR, we found that PtrMYB021 was expressed primarily in xylem tissues. When expressed in Arabidopsis under the control of PtrCesA8, but not the 35S promoter, PtrMYB021 increased secondary cell wall thickness, which is likely caused by increased lignification as well as changes in cell wall carbohydrate composition. Consistent with this, elevated expression of lignin and cellulose biosynthetic genes were observed in the transgenic plants. Finally, when expressed in Arabidopsis protoplasts as fusion proteins to the Gal4 DNA binding domain, PtrMYB021 activated the reporter gene Gal4-GUS. In summary, our results suggest that PtrMYB021 is a transcriptional activator, and spatially and temporally restricted expression of PtrMYB021 in Arabidopsis regulates secondary cell wall formation by activating a subset of secondary cell wall biosynthesis genes.},
doi = {10.1007/s12374-016-0438-0},
journal = {Journal of Plant Biology},
number = 1,
volume = 59,
place = {United States},
year = {Tue Feb 02 00:00:00 EST 2016},
month = {Tue Feb 02 00:00:00 EST 2016}
}
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Works referencing / citing this record:
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