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Title: Ordering in bio-inorganic hybrid nanomaterials probed by in situ scanning transmission X-ray microscopy

Here, phospholipid bilayer coated Si nanowires are one-dimensional (1D) composites that provide versatile bio-nanoelectronic functionality via incorporation of a wide variety of biomolecules into the phospholipid matrix. The physiochemical behaviour of the phospholipid bilayer is strongly dependent on its structure and, as a consequence, substantial modelling and experimental efforts have been directed at the structural characterization of supported bilayers and unsupported phospholipid vesicles; nonetheless, the experimental studies conducted to date have exclusively involved volume-averaged techniques, which do not allow for the assignment of spatially resolved structural variations that could critically impact the performance of the 1D phospholipid-Si NW composites. In this manuscript, we use scanning transmission X-ray microscopy (STXM) to probe bond orientation and bilayer thickness as a function of position with a spatial resolution of ~30 nm for Δ9-cis 1,2-dioleoyl-sn-glycero-3-phosphocholine layers prepared Si NWs. When coupled with small angle X-ray scattering measurements, the STXM data reveal structural motifs of the Si NWs that give rise to multi-bilayer formation and enable assignment of the orientation of specific bonds known to affect the order and rigidity of phospholipid bilayers.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [1] ;  [3] ;  [1] ;  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Berkeley, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Report Number(s):
LLNL-JRNL-663215
Journal ID: ISSN 2040-3364; NANOHL
Grant/Contract Number:
AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
Nanoscale
Additional Journal Information:
Journal Volume: 7; Journal Issue: 21; Journal ID: ISSN 2040-3364
Publisher:
Royal Society of Chemistry
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1313546

Lee, Jonathan R. I., Bagge-Hansen, Michael, Tunuguntla, Ramya, Kim, Kyunghoon, Bangar, Mangesh, Willey, Trevor M., Tran, Ich C., Kilcoyne, David A., Noy, Aleksandr, and van Buuren, Tony. Ordering in bio-inorganic hybrid nanomaterials probed by in situ scanning transmission X-ray microscopy. United States: N. p., Web. doi:10.1039/C5NR00622H.
Lee, Jonathan R. I., Bagge-Hansen, Michael, Tunuguntla, Ramya, Kim, Kyunghoon, Bangar, Mangesh, Willey, Trevor M., Tran, Ich C., Kilcoyne, David A., Noy, Aleksandr, & van Buuren, Tony. Ordering in bio-inorganic hybrid nanomaterials probed by in situ scanning transmission X-ray microscopy. United States. doi:10.1039/C5NR00622H.
Lee, Jonathan R. I., Bagge-Hansen, Michael, Tunuguntla, Ramya, Kim, Kyunghoon, Bangar, Mangesh, Willey, Trevor M., Tran, Ich C., Kilcoyne, David A., Noy, Aleksandr, and van Buuren, Tony. 2015. "Ordering in bio-inorganic hybrid nanomaterials probed by in situ scanning transmission X-ray microscopy". United States. doi:10.1039/C5NR00622H. https://www.osti.gov/servlets/purl/1313546.
@article{osti_1313546,
title = {Ordering in bio-inorganic hybrid nanomaterials probed by in situ scanning transmission X-ray microscopy},
author = {Lee, Jonathan R. I. and Bagge-Hansen, Michael and Tunuguntla, Ramya and Kim, Kyunghoon and Bangar, Mangesh and Willey, Trevor M. and Tran, Ich C. and Kilcoyne, David A. and Noy, Aleksandr and van Buuren, Tony},
abstractNote = {Here, phospholipid bilayer coated Si nanowires are one-dimensional (1D) composites that provide versatile bio-nanoelectronic functionality via incorporation of a wide variety of biomolecules into the phospholipid matrix. The physiochemical behaviour of the phospholipid bilayer is strongly dependent on its structure and, as a consequence, substantial modelling and experimental efforts have been directed at the structural characterization of supported bilayers and unsupported phospholipid vesicles; nonetheless, the experimental studies conducted to date have exclusively involved volume-averaged techniques, which do not allow for the assignment of spatially resolved structural variations that could critically impact the performance of the 1D phospholipid-Si NW composites. In this manuscript, we use scanning transmission X-ray microscopy (STXM) to probe bond orientation and bilayer thickness as a function of position with a spatial resolution of ~30 nm for Δ9-cis 1,2-dioleoyl-sn-glycero-3-phosphocholine layers prepared Si NWs. When coupled with small angle X-ray scattering measurements, the STXM data reveal structural motifs of the Si NWs that give rise to multi-bilayer formation and enable assignment of the orientation of specific bonds known to affect the order and rigidity of phospholipid bilayers.},
doi = {10.1039/C5NR00622H},
journal = {Nanoscale},
number = 21,
volume = 7,
place = {United States},
year = {2015},
month = {4}
}