Manufacturing demonstration of microbially mediated zinc sulfide nanoparticles in pilot-plant scale reactors
Abstract
The thermophilic anaerobic metal-reducing bacterium Thermoanaerobacter sp. X513 efficiently produces zinc sulfide (ZnS) nanoparticles (NPs) in laboratory-scale ( ≤24-L) reactors. To determine whether this process can be up-scaled and adapted for pilot-plant production while maintaining NP yield and quality, a series of meso-scale experiments were performed using 100-l and 900-l reactors. Pasteurization and N2-sparging replaced autoclaving and boiling for deoxygenating media in the transition from small-scale to pilot-plant reactors. Consecutive 100-L batches using new or recycled media produced ZnS NPs with highly reproducible ~2 nm average crystallite size (ACS) and yields of ~0.5g L-1, similar to small-scale batches. The 900-L pilot plant reactor produced ~ 320 g ZnS without process optimization or replacement of used medium; this quantity would be sufficient to form a ZnS thin film with ~120 nm thickness over 0.5 m width 13 km length. At all scales, the bacteria produced significant amounts of acetic, lactic and formic acids, which could be neutralized by the controlled addition of sodium hydroxide without the use of an organic pH buffer, eliminating 98% of the buffer chemical costs. In conclusion, the final NP products were characterized using XRD, ICP-OES, FTIR, DLS, and C/N analyses, which confirmed the growth medium withoutmore »
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Fusion Materials for Nuclear Systems Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Energy and Transportation Science Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Materials Science and Technology Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). High Temperature Materials Lab. (HTML)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1311219
- Grant/Contract Number:
- AC05-00OR22725; CPS 24762
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied Microbiology and Biotechnology
- Additional Journal Information:
- Journal Volume: 100; Journal Issue: 18; Journal ID: ISSN 0175-7598
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Pilot plant reactor; Microbially mediated manufacturing; Zinc sulfide; nanoparticles; Scalability
Citation Formats
Moon, Ji-Won, Phelps, Tommy J., Fitzgerald Jr, Curtis L., Lind, Randall F., Elkins, James G., Jang, Gyoung Gug, Joshi, Pooran C., Kidder, Michelle, Armstrong, Beth L., Watkins, Thomas R., Ivanov, Ilia N., and Graham, David E. Manufacturing demonstration of microbially mediated zinc sulfide nanoparticles in pilot-plant scale reactors. United States: N. p., 2016.
Web. doi:10.1007/s00253-016-7556-y.
Moon, Ji-Won, Phelps, Tommy J., Fitzgerald Jr, Curtis L., Lind, Randall F., Elkins, James G., Jang, Gyoung Gug, Joshi, Pooran C., Kidder, Michelle, Armstrong, Beth L., Watkins, Thomas R., Ivanov, Ilia N., & Graham, David E. Manufacturing demonstration of microbially mediated zinc sulfide nanoparticles in pilot-plant scale reactors. United States. https://doi.org/10.1007/s00253-016-7556-y
Moon, Ji-Won, Phelps, Tommy J., Fitzgerald Jr, Curtis L., Lind, Randall F., Elkins, James G., Jang, Gyoung Gug, Joshi, Pooran C., Kidder, Michelle, Armstrong, Beth L., Watkins, Thomas R., Ivanov, Ilia N., and Graham, David E. Wed .
"Manufacturing demonstration of microbially mediated zinc sulfide nanoparticles in pilot-plant scale reactors". United States. https://doi.org/10.1007/s00253-016-7556-y. https://www.osti.gov/servlets/purl/1311219.
@article{osti_1311219,
title = {Manufacturing demonstration of microbially mediated zinc sulfide nanoparticles in pilot-plant scale reactors},
author = {Moon, Ji-Won and Phelps, Tommy J. and Fitzgerald Jr, Curtis L. and Lind, Randall F. and Elkins, James G. and Jang, Gyoung Gug and Joshi, Pooran C. and Kidder, Michelle and Armstrong, Beth L. and Watkins, Thomas R. and Ivanov, Ilia N. and Graham, David E.},
abstractNote = {The thermophilic anaerobic metal-reducing bacterium Thermoanaerobacter sp. X513 efficiently produces zinc sulfide (ZnS) nanoparticles (NPs) in laboratory-scale ( ≤24-L) reactors. To determine whether this process can be up-scaled and adapted for pilot-plant production while maintaining NP yield and quality, a series of meso-scale experiments were performed using 100-l and 900-l reactors. Pasteurization and N2-sparging replaced autoclaving and boiling for deoxygenating media in the transition from small-scale to pilot-plant reactors. Consecutive 100-L batches using new or recycled media produced ZnS NPs with highly reproducible ~2 nm average crystallite size (ACS) and yields of ~0.5g L-1, similar to small-scale batches. The 900-L pilot plant reactor produced ~ 320 g ZnS without process optimization or replacement of used medium; this quantity would be sufficient to form a ZnS thin film with ~120 nm thickness over 0.5 m width 13 km length. At all scales, the bacteria produced significant amounts of acetic, lactic and formic acids, which could be neutralized by the controlled addition of sodium hydroxide without the use of an organic pH buffer, eliminating 98% of the buffer chemical costs. In conclusion, the final NP products were characterized using XRD, ICP-OES, FTIR, DLS, and C/N analyses, which confirmed the growth medium without organic buffer enhanced the ZnS NP properties by reducing carbon and nitrogen surface coatings and supporting better dispersivity with similar ACS.},
doi = {10.1007/s00253-016-7556-y},
journal = {Applied Microbiology and Biotechnology},
number = 18,
volume = 100,
place = {United States},
year = {Wed Apr 27 00:00:00 EDT 2016},
month = {Wed Apr 27 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 17 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Biological Synthesis of Semiconductor Zinc Sulfide Nanoparticles by Immobilized Rhodobacter sphaeroides
journal, June 2006
- Bai, Hong-Juan; Zhang, Zhao-Ming; Gong, Jun
- Biotechnology Letters, Vol. 28, Issue 14
Nanostructured ZnS:Ni2+ Photocatalysts Prepared by Ultrasonic Spray Pyrolysis
journal, July 2008
- Bang, Jin Ho; Helmich, Richard J.; Suslick, Kenneth S.
- Advanced Materials, Vol. 20, Issue 13, p. 2599-2603
ZnO and ZnS Nanostructures: Ultraviolet-Light Emitters, Lasers, and Sensors
journal, November 2009
- Fang, Xiaosheng; Bando, Yoshio; Gautam, Ujjal K.
- Critical Reviews in Solid State and Materials Sciences, Vol. 34, Issue 3-4
ZnS nanostructures: From synthesis to applications
journal, February 2011
- Fang, Xiaosheng; Zhai, Tianyou; Gautam, Ujjal K.
- Progress in Materials Science, Vol. 56, Issue 2
Palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen
journal, May 2011
- Hennebel, Tom; Van Nevel, Sam; Verschuere, Stephanie
- Applied Microbiology and Biotechnology, Vol. 91, Issue 5
A biomimetic approach towards synthesis of zinc oxide nanoparticles
journal, March 2012
- Jain, Navin; Bhargava, Arpit; Tarafdar, Jagadish C.
- Applied Microbiology and Biotechnology, Vol. 97, Issue 2
Size tunable elemental copper nanoparticles: extracellular synthesis by thermoanaerobic bacteria and capping molecules
journal, January 2015
- Jang, Gyoung Gug; Jacobs, Christopher B.; Gresback, Ryan G.
- Journal of Materials Chemistry C, Vol. 3, Issue 3
In situ capping for size control of monochalcogenide (ZnS, CdS and SnS) nanocrystals produced by anaerobic metal-reducing bacteria
journal, July 2015
- Jang, Gyoung Gug; Jacobs, Christopher B.; Ivanov, Ilia N.
- Nanotechnology, Vol. 26, Issue 32
Ligand-Controlling Synthesis and Ordered Assembly of ZnS Nanorods and Nanodots
journal, October 2004
- Li, Yunchao; Li, Xiaohong; Yang, Chunhe
- The Journal of Physical Chemistry B, Vol. 108, Issue 41
Extension of the benzyl alcohol route to metal sulfides: “nonhydrolytic” thio sol–gel synthesis of ZnS and SnS2
journal, January 2011
- Ludi, Bettina; Olliges-Stadler, Inga; Rossell, Marta D.
- Chemical Communications, Vol. 47, Issue 18
Long-term solid-phase fate of co-precipitated U(VI)-Fe(III) following biological iron reduction by Thermoanaerobacter
journal, September 2012
- Madden, A. S.; Swindle, A. L.; Beazley, M. J.
- American Mineralogist, Vol. 97, Issue 10
Facile production of ZnS quantum dot nanoparticles by Saccharomyces cerevisiae MTCC 2918
journal, January 2014
- Sandana Mala, John Geraldine; Rose, Chellan
- Journal of Biotechnology, Vol. 170
Controllable synthesis, characterization and optical properties of ZnS:Mn nanoparticles as a novel biosensor
journal, August 2009
- Mohagheghpour, E.; Rabiee, M.; Moztarzadeh, F.
- Materials Science and Engineering: C, Vol. 29, Issue 6
Scalable economic extracellular synthesis of CdS nanostructured particles by a non-pathogenic thermophile
journal, September 2013
- Moon, Ji-Won; Ivanov, Ilia N.; Duty, Chad E.
- Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 11
Large-scale production of magnetic nanoparticles using bacterial fermentation
journal, June 2010
- Moon, Ji-Won; Rawn, Claudia J.; Rondinone, Adam J.
- Journal of Industrial Microbiology & Biotechnology, Vol. 37, Issue 10
Crystallite Sizes and Lattice Parameters of Nano-Biomagnetite Particles
journal, December 2010
- Moon, Ji-Won; Rawn, Claudia J.; Rondinone, Adam J.
- Journal of Nanoscience and Nanotechnology, Vol. 10, Issue 12
Microbial preparation of metal-substituted magnetite nanoparticles
journal, July 2007
- Moon, Ji-Won; Roh, Yul; Lauf, Robert J.
- Journal of Microbiological Methods, Vol. 70, Issue 1, p. 150-158
Microbial formation of lanthanide-substituted magnetites by Thermoanaerobacter sp. TOR-39
journal, August 2007
- Moon, Ji-Won; Roh, Yul; Yeary, Lucas W.
- Extremophiles, Vol. 11, Issue 6, p. 859-867
Extracellular Proteins Limit the Dispersal of Biogenic Nanoparticles
journal, June 2007
- Moreau, J. W.; Weber, P. K.; Martin, M. C.
- Science, Vol. 316, Issue 5831
Sulfide product inhibition of Desulfovibrio desulfuricans in batch and continuous cultures
journal, February 1995
- Okabe, S.; Nielsen, P. H.; Jones, W. L.
- Water Research, Vol. 29, Issue 2
Advances in microbial biosynthesis of metal nanoparticles
journal, August 2015
- Park, Tae Jung; Lee, Kyoung G.; Lee, Sang Yup
- Applied Microbiology and Biotechnology, Vol. 100, Issue 2
Isolation and Characterization of Metal-Reducing Thermoanaerobacter Strains from Deep Subsurface Environments of the Piceance Basin, Colorado
journal, December 2002
- Roh, Y.; Liu, S. V.; Li, G.
- Applied and Environmental Microbiology, Vol. 68, Issue 12
Comparative study of MnO2 nanoparticle synthesis by marine bacterium Saccharophagus degradans and yeast Saccharomyces cerevisiae
journal, April 2015
- Salunke, Bipinchandra K.; Sawant, Shailesh S.; Lee, Sang-Ill
- Applied Microbiology and Biotechnology, Vol. 99, Issue 13
Applications of biosynthesized metallic nanoparticles – A review
journal, October 2014
- Schröfel, Adam; Kratošová, Gabriela; Šafařík, Ivo
- Acta Biomaterialia, Vol. 10, Issue 10
Synthesis and fluorescence properties of pure and metal-doped spherical ZnS particles from EDTA–metal complexes
journal, January 2008
- Song, Haiyan; Leem, Young-Min; Kim, Byoung-Gyu
- Journal of Physics and Chemistry of Solids, Vol. 69, Issue 1, p. 153-160
Community Analysis of Plant Biomass-Degrading Microorganisms from Obsidian Pool, Yellowstone National Park
journal, October 2014
- Vishnivetskaya, Tatiana A.; Hamilton-Brehm, Scott D.; Podar, Mircea
- Microbial Ecology, Vol. 69, Issue 2
Visible emission characteristics from different defects of ZnS nanocrystals
journal, January 2011
- Wang, Xiuli; Shi, Jianying; Feng, Zhaochi
- Physical Chemistry Chemical Physics, Vol. 13, Issue 10
Generation and Optical Properties of Monodisperse Wurtzite-Type ZnS Microspheres
journal, August 2006
- Wu, Qingzhi; Cao, Huaqiang; Zhang, Sichun
- Inorganic Chemistry, Vol. 45, Issue 18
Shape and Phase Control of ZnS Nanocrystals: Template Fabrication of Wurtzite ZnS Single-Crystal Nanosheets and ZnO Flake-like Dendrites from a Lamellar Molecular Precursor ZnS·(NH2CH2CH2NH2)0.5
journal, February 2002
- Yu, S. -H.; Yoshimura, M.
- Advanced Materials, Vol. 14, Issue 4
Works referencing / citing this record:
Nanotechnology in Plant Science: To Make a Long Story Short
journal, May 2019
- Sanzari, Ilaria; Leone, Antonietta; Ambrosone, Alfredo
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Improved ZnS nanoparticle properties through sequential NanoFermentation
journal, August 2018
- Moon, Ji-Won; Eskelsen, Jeremy R.; Ivanov, Ilia N.
- Applied Microbiology and Biotechnology, Vol. 102, Issue 19
A review on the biosynthesis of metal and metal salt nanoparticles by microbes
journal, January 2019
- Gahlawat, Geeta; Choudhury, Anirban Roy
- RSC Advances, Vol. 9, Issue 23
Nanotechnology in Plant Science: To Make a Long Story Short
journal, May 2019
- Sanzari, Ilaria; Leone, Antonietta; Ambrosone, Alfredo
- Frontiers in Bioengineering and Biotechnology, Vol. 7