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Title: Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase

Abstract

Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens’ lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Furthermore, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.

Authors:
 [1];  [1];  [2];  [3];  [1];  [1];  [4];  [1];  [2]; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Univ. of Wisconsin, Madison, WI (United States)
  3. Appalachian State Univ., Boone, NC (United States)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); Montclair State Univ., Montclair, NJ (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1307422
Alternate Identifier(s):
OSTI ID: 1341631
Report Number(s):
BNL-112598-2016-JA
Journal ID: ISSN 2041-1723; ncomms11989
Grant/Contract Number:  
FC02-07ER64494; SC00112704
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Biofuels; Molecular engineering in plants; Transgenic plants

Citation Formats

Cai, Yuanheng, Zhang, Kewei, Kim, Hoon, Hou, Guichuan, Zhang, Xuebin, Yang, Huijun, Feng, Huan, Miller, Lisa, Ralph, John, and Liu, Chang -Jun. Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase. United States: N. p., 2016. Web. doi:10.1038/ncomms11989.
Cai, Yuanheng, Zhang, Kewei, Kim, Hoon, Hou, Guichuan, Zhang, Xuebin, Yang, Huijun, Feng, Huan, Miller, Lisa, Ralph, John, & Liu, Chang -Jun. Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase. United States. doi:10.1038/ncomms11989.
Cai, Yuanheng, Zhang, Kewei, Kim, Hoon, Hou, Guichuan, Zhang, Xuebin, Yang, Huijun, Feng, Huan, Miller, Lisa, Ralph, John, and Liu, Chang -Jun. Tue . "Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase". United States. doi:10.1038/ncomms11989. https://www.osti.gov/servlets/purl/1307422.
@article{osti_1307422,
title = {Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase},
author = {Cai, Yuanheng and Zhang, Kewei and Kim, Hoon and Hou, Guichuan and Zhang, Xuebin and Yang, Huijun and Feng, Huan and Miller, Lisa and Ralph, John and Liu, Chang -Jun},
abstractNote = {Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens’ lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Furthermore, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.},
doi = {10.1038/ncomms11989},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {6}
}

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Cited by: 5 works
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    Works referencing / citing this record:

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