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Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the 18O-cholesterol and 15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of 18O-cholesterol and 15N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the 18O-enriched regions suggest that these 18O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.
Authors:
 [1] ;  [2] ;  [1]
  1. Univ. of Illinois, Urbana, IL (United States). Dept. of Chemical and Biomolecular Engineering
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Glenn T. Seaborg Institute
Publication Date:
Report Number(s):
LLNL-JRNL-680575
Journal ID: ISSN 1934-8630; BJIOBN
Grant/Contract Number:
AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
Biointerphases
Additional Journal Information:
Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1934-8630
Publisher:
AIP
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; cell membranes; secondary ion mass spectroscopy; lipids; acids; image analysis
OSTI Identifier:
1305902

Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L.. Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell. United States: N. p., Web. doi:10.1116/1.4939681.
Yeager, Ashley N., Weber, Peter K., & Kraft, Mary L.. Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell. United States. doi:10.1116/1.4939681.
Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L.. 2016. "Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell". United States. doi:10.1116/1.4939681. https://www.osti.gov/servlets/purl/1305902.
@article{osti_1305902,
title = {Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell},
author = {Yeager, Ashley N. and Weber, Peter K. and Kraft, Mary L.},
abstractNote = {Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the 18O-cholesterol and 15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of 18O-cholesterol and 15N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the 18O-enriched regions suggest that these 18O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.},
doi = {10.1116/1.4939681},
journal = {Biointerphases},
number = 2,
volume = 11,
place = {United States},
year = {2016},
month = {1}
}