Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Yeager, Ashley N.; Weber, Peter K.; Kraft, Mary L.

doi:10.1116/1.4939681

Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Abstract

Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the ¹⁸O-cholesterol and ¹⁵N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of ¹⁸O-cholesterol and ¹⁵N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the ¹⁸O-enriched regions suggest that these ¹⁸O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.

Authors:

Yeager, Ashley N. ^[1]; Weber, Peter K. ^[2]; Kraft, Mary L. ^[1]

Univ. of Illinois, Urbana, IL (United States). Dept. of Chemical and Biomolecular Engineering
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Glenn T. Seaborg Institute

Publication Date:: Fri Jan 08 00:00:00 EST 2016

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1305902

Alternate Identifier(s):: OSTI ID: 1421226

Report Number(s):: LLNL-JRNL-680575
Journal ID: ISSN 1934-8630; BJIOBN

Grant/Contract Number:: AC52-07NA27344

Resource Type:: Accepted Manuscript

Journal Name:: Biointerphases

Additional Journal Information:: Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1934-8630

Publisher:: AIP

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; cell membranes; secondary ion mass spectroscopy; lipids; acids; image analysis

Citation Formats


                    Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell.  United States: N. p., 2016. 
Web.  doi:10.1116/1.4939681.

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                    Yeager, Ashley N., Weber, Peter K., & Kraft, Mary L. Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell.  United States.  https://doi.org/10.1116/1.4939681

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                    Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Fri .  
"Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell".  United States.  https://doi.org/10.1116/1.4939681.  https://www.osti.gov/servlets/purl/1305902.

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@article{osti_1305902,

  title        = {Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell},

  author       = {Yeager, Ashley N. and Weber, Peter K. and Kraft, Mary L.},

  abstractNote = {Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the 18O-cholesterol and 15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of 18O-cholesterol and 15N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the 18O-enriched regions suggest that these 18O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.},

  doi          = {10.1116/1.4939681},

  journal      = {Biointerphases},

  number       = 2,

  volume       = 11,

  place        = {United States},

  year         = {Fri Jan 08 00:00:00 EST 2016},

  month        = {Fri Jan 08 00:00:00 EST 2016}

}

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Works referencing / citing this record:

Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It
journal, January 2017

Kraft, Mary L.
Frontiers in Cell and Developmental Biology, Vol. 4
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Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Abstract

Citation Formats

Bioactive sphingolipids: metabolism and function journal, April 2009

OSBP Is a Cholesterol-Regulated Scaffolding Protein in Control of ERK 1/2 Activation journal, March 2005

Effects of a sphingolipid synthesis inhibitor on membrane transport through the secretory pathway journal, April 1992

Lipids and Cholesterol as Regulators of Traffic in the Endomembrane System journal, April 2010

The pathophysiology and mechanisms of NP-C disease journal, October 2004

Natural ceramide is unable to escape the lysosome, in contrast to a fluorescent analogue journal, April 1998

Direct chemical evidence for sphingolipid domains in the plasma membranes of fibroblasts journal, January 2013

Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids journal, April 2015

Advances in Imaging Secondary Ion Mass Spectrometry for Biological Samples journal, June 2009

Imaging lipids with secondary ion mass spectrometry journal, August 2014

Separation of cell organelles in density gradients based on their permeability characteristics journal, May 1988

Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles journal, March 2011

Spontaneous and Protein-mediated Sterol Transfer between Intracellular Membranes journal, July 1996

Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications journal, February 2015

Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry journal, October 2011

TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C 60 ) Primary Ions journal, March 2007

Imaging and 3D Elemental Characterization of Intact Bacterial Spores by High-Resolution Secondary Ion Mass Spectrometry journal, August 2008

TOF-SIMS 3D Imaging of Native and Non-Native Species within HeLa Cells journal, November 2013

ToF-SIMS Depth Profiling of Cells: z -Correction, 3D Imaging, and Sputter Rate of Individual NIH/3T3 Fibroblasts journal, June 2012

The Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry journal, July 2007

Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation: 3D mass spectral imaging of HeLa-M cells journal, March 2011

Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS journal, June 2016

A stereodivergent synthesis of D-erythro-sphingosine and D-threo-sphingosine from L-serine journal, September 1988

Synthesis of Borondipyrromethene (BODIPY)-Labeled Sphingosine Derivatives by Cross-metathesis Reaction journal, March 2007

THE FORMATION OF CHOLESTEROL FROM i-CHOLESTERYL METHYL ETHER journal, January 1948

A high-level 3D visualization API for Java and ImageJ journal, May 2010

Cellular cholesterol trafficking and compartmentalization journal, February 2008

Multi-isotope imaging mass spectrometry quantifies stem cell division and metabolism journal, January 2012

Lipid Droplets and Cellular Lipid Metabolism journal, July 2012

Phospholipids and lipid droplets journal, March 2013

Not Just Fat: The Structure and Function of the Lipid Droplet journal, January 2011

Intracellular sterol dynamics journal, July 2009

Modulation of membrane function by cholesterol journal, October 1991

Cholesterol Distribution in Living Cells: Fluorescence Imaging Using Dehydroergosterol as a Fluorescent Cholesterol Analog journal, October 1998

Plasma Membranes Contain Half the Phospholipid and 90% of the Cholesterol and Sphingomyelin in Cultured Human Fibroblasts journal, March 1989

Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway journal, April 2003

Transport of 13C-oleate in adipocytes measured using multi imaging mass spectrometry journal, November 2004

High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis journal, October 2014

Topographic Correction of 3D SIMS Images journal, September 1997

Protocols for Three-Dimensional Molecular Imaging Using Mass Spectrometry journal, August 2007

Three-dimensional molecular imaging using mass spectrometry and atomic force microscopy journal, December 2008

Combined SIMS-SPM instrument for high sensitivity and high-resolution elemental 3D analysis: Combined SIMS-SPM instrument for high-resolution elemental 3D analysis journal, April 2012

Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It journal, January 2017

SIMS imaging in neurobiology and cell biology journal, January 2019

Bioactive sphingolipids: metabolism and function
journal, April 2009

OSBP Is a Cholesterol-Regulated Scaffolding Protein in Control of ERK 1/2 Activation
journal, March 2005

Effects of a sphingolipid synthesis inhibitor on membrane transport through the secretory pathway
journal, April 1992

Lipids and Cholesterol as Regulators of Traffic in the Endomembrane System
journal, April 2010

The pathophysiology and mechanisms of NP-C disease
journal, October 2004

Natural ceramide is unable to escape the lysosome, in contrast to a fluorescent analogue
journal, April 1998

Direct chemical evidence for sphingolipid domains in the plasma membranes of fibroblasts
journal, January 2013

Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids
journal, April 2015

Advances in Imaging Secondary Ion Mass Spectrometry for Biological Samples
journal, June 2009

Imaging lipids with secondary ion mass spectrometry
journal, August 2014

Separation of cell organelles in density gradients based on their permeability characteristics
journal, May 1988

Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles
journal, March 2011

Spontaneous and Protein-mediated Sterol Transfer between Intracellular Membranes
journal, July 1996

Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications
journal, February 2015

Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry
journal, October 2011

TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C ₆₀ ) Primary Ions
journal, March 2007

Imaging and 3D Elemental Characterization of Intact Bacterial Spores by High-Resolution Secondary Ion Mass Spectrometry
journal, August 2008

TOF-SIMS 3D Imaging of Native and Non-Native Species within HeLa Cells
journal, November 2013

ToF-SIMS Depth Profiling of Cells: z -Correction, 3D Imaging, and Sputter Rate of Individual NIH/3T3 Fibroblasts
journal, June 2012

The Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry
journal, July 2007

Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation: 3D mass spectral imaging of HeLa-M cells
journal, March 2011

Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS
journal, June 2016

A stereodivergent synthesis of D-erythro-sphingosine and D-threo-sphingosine from L-serine
journal, September 1988

Synthesis of Borondipyrromethene (BODIPY)-Labeled Sphingosine Derivatives by Cross-metathesis Reaction
journal, March 2007

THE FORMATION OF CHOLESTEROL FROM i-CHOLESTERYL METHYL ETHER
journal, January 1948

A high-level 3D visualization API for Java and ImageJ
journal, May 2010

Cellular cholesterol trafficking and compartmentalization
journal, February 2008

Multi-isotope imaging mass spectrometry quantifies stem cell division and metabolism
journal, January 2012

Lipid Droplets and Cellular Lipid Metabolism
journal, July 2012

Phospholipids and lipid droplets
journal, March 2013

Not Just Fat: The Structure and Function of the Lipid Droplet
journal, January 2011

Intracellular sterol dynamics
journal, July 2009

Modulation of membrane function by cholesterol
journal, October 1991

Cholesterol Distribution in Living Cells: Fluorescence Imaging Using Dehydroergosterol as a Fluorescent Cholesterol Analog
journal, October 1998

Plasma Membranes Contain Half the Phospholipid and 90% of the Cholesterol and Sphingomyelin in Cultured Human Fibroblasts
journal, March 1989

Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway
journal, April 2003

Transport of ¹³C-oleate in adipocytes measured using multi imaging mass spectrometry
journal, November 2004

High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis
journal, October 2014

Topographic Correction of 3D SIMS Images
journal, September 1997

Protocols for Three-Dimensional Molecular Imaging Using Mass Spectrometry
journal, August 2007

Three-dimensional molecular imaging using mass spectrometry and atomic force microscopy
journal, December 2008

Combined SIMS-SPM instrument for high sensitivity and high-resolution elemental 3D analysis: Combined SIMS-SPM instrument for high-resolution elemental 3D analysis
journal, April 2012

Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It
journal, January 2017

SIMS imaging in neurobiology and cell biology
journal, January 2019