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Title: The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy

Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentrations of ionic liquids, has been challenging. In the present work the 13C, 15N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid-protein interactions. Effect of an ionic liquid (1-butyl-3-methylimidazolium bromide, [C4-mim]Br) on GB1was studied over a wide range of the ionic liquid concentrations (0.6-3.5 M, which corresponds to 10-60% v/v). Interactions between GB1 and [C4-mim]Br were observed from changes in the chemical shifts of the protein backbone as well as the changes in 15N ps-ns dynamics and rotational correlation times. Site-specific interactions between the protein and [C4-mim]Br were assigned using 3D methods under HR-MAS conditions. Furthermore, HR-MAS NMR is a viable tool that could aid in elucidation of molecular mechanisms of ionic liquid-protein interactions.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [3]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States); Boise State Univ., Boise, ID (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Univ. of Wyoming, Laramie, WY (United States)
  4. Texas Christian Univ., Fort Worth, TX (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-68988
Journal ID: ISSN 2405-5808
Grant/Contract Number:
AC36-08GO28308
Type:
Published Article
Journal Name:
Biochemistry and Biophysics Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: C; Journal ID: ISSN 2405-5808
Publisher:
Elsevier
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; imidazolium ionic liquid; GB1; ionic liquid-protein interaction; HR-MAS NMR
OSTI Identifier:
1305434
Alternate Identifier(s):
OSTI ID: 1373678