The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy
Abstract
Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentrations of ionic liquids, has been challenging. In the present work the 13C, 15N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid-protein interactions. Effect of an ionic liquid (1-butyl-3-methylimidazolium bromide, [C4-mim]Br) on GB1was studied over a wide range of the ionic liquid concentrations (0.6-3.5 M, which corresponds to 10-60% v/v). Interactions between GB1 and [C4-mim]Br were observed from changes in the chemical shifts of the protein backbone as well as the changes in 15N ps-ns dynamics and rotational correlation times. Site-specific interactions between the protein and [C4-mim]Br were assigned using 3D methods under HR-MAS conditions. Furthermore, HR-MAS NMR is a viable tool that could aid in elucidation of molecular mechanisms of ionic liquid-protein interactions.
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1305434
- Alternate Identifier(s):
- OSTI ID: 1373678
- Report Number(s):
- NREL/JA-5100-68988
Journal ID: ISSN 2405-5808; S2405580816301406; PII: S2405580816301406
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biochemistry and Biophysics Reports
- Additional Journal Information:
- Journal Name: Biochemistry and Biophysics Reports Journal Volume: 8 Journal Issue: C; Journal ID: ISSN 2405-5808
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; imidazolium ionic liquid; GB1; ionic liquid-protein interaction; HR-MAS NMR
Citation Formats
Warner, Lisa, Gjersing, Erica, Follett, Shelby E., Elliott, K. Wade, Dzyuba, Sergei V., and Varga, Krisztina. The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy. Netherlands: N. p., 2016.
Web. doi:10.1016/j.bbrep.2016.08.009.
Warner, Lisa, Gjersing, Erica, Follett, Shelby E., Elliott, K. Wade, Dzyuba, Sergei V., & Varga, Krisztina. The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy. Netherlands. https://doi.org/10.1016/j.bbrep.2016.08.009
Warner, Lisa, Gjersing, Erica, Follett, Shelby E., Elliott, K. Wade, Dzyuba, Sergei V., and Varga, Krisztina. Thu .
"The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy". Netherlands. https://doi.org/10.1016/j.bbrep.2016.08.009.
@article{osti_1305434,
title = {The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy},
author = {Warner, Lisa and Gjersing, Erica and Follett, Shelby E. and Elliott, K. Wade and Dzyuba, Sergei V. and Varga, Krisztina},
abstractNote = {Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentrations of ionic liquids, has been challenging. In the present work the 13C, 15N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid-protein interactions. Effect of an ionic liquid (1-butyl-3-methylimidazolium bromide, [C4-mim]Br) on GB1was studied over a wide range of the ionic liquid concentrations (0.6-3.5 M, which corresponds to 10-60% v/v). Interactions between GB1 and [C4-mim]Br were observed from changes in the chemical shifts of the protein backbone as well as the changes in 15N ps-ns dynamics and rotational correlation times. Site-specific interactions between the protein and [C4-mim]Br were assigned using 3D methods under HR-MAS conditions. Furthermore, HR-MAS NMR is a viable tool that could aid in elucidation of molecular mechanisms of ionic liquid-protein interactions.},
doi = {10.1016/j.bbrep.2016.08.009},
journal = {Biochemistry and Biophysics Reports},
number = C,
volume = 8,
place = {Netherlands},
year = {Thu Dec 01 00:00:00 EST 2016},
month = {Thu Dec 01 00:00:00 EST 2016}
}
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