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Title: Sugarcane transgenics expressing MYB transcription factors show improved glucose release

Abstract

In this study, sugarcane, a tropical C4 perennial crop, is capable of producing 30-100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and can provide a significant source of biomass for the production of second-generation bioethanol. As a result, MYB31 and MYB42 were cloned from maize and expressed in sugarcane with and without the UTR sequences. The cloned sequences were 98 and 99 % identical to the published nucleotide sequences. The inclusion of the UTR sequences did not affect any of the parameters tested. There was little difference in plant height and the number of internodes of the MYB-overexpressing sugarcane plants when compared with controls. MYB transgene expression determined by qPCR exhibited continued expression in young and maturing internodes. MYB31 downregulated more genes within the lignin biosynthetic pathway than MYB42. MYB31 and MYB42 expression resulted in decreased lignin content in some lines. All MYB42 plants further analyzed showed significant increases in glucose release by enzymatic hydrolysis in 72 h, whereas only two MYB31 plants released more glucose than control plants. This correlated directly with a significant decrease in acid-insoluble lignin. Soluble sucrose content of the MYB42 transgenic plants did notmore » vary compared to control plants. In conclusion, this study demonstrates the use of MYB transcription factors to improve the production of bioethanol from sugarcane bagasse remaining after sugar extraction.« less

Authors:
 [1];  [2];  [3];  [4]; ORCiD logo [1]
  1. Syracuse Univ., Syracuse, NY (United States). Dept. of Biology
  2. Queensland Univ. of Technology, Brisbane (Australia). Center for Tropical Crops and Biocommodities
  3. Univ. of Wisconsin, Madison, WI (United States). US Dept. of Energy, Great Lakes Bioenergy Research Center (GLBRC), Wisconsin Energy Inst. and Dept. of Biological System Engineering
  4. Univ. of Wisconsin, Madison, WI (United States). US Dept. of Energy, Great Lakes Bioenergy Research Center (GLBRC), Wisconsin Energy Inst. and Dept. of Biochemistry
Publication Date:
Research Org.:
Univ. of Wisconsin System, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1299148
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; sugarcane bagasse; bioethanol; biofuel; lignin modification; cinnamyl alcohol-dehydrogenase; state 2d nmr; lignin biosynthesis; biofuel production; untranslated regions; chemical-composition; acid pretreatment; lignification; promoter; plants

Citation Formats

Poovaiah, Charleson R., Bewg, William P., Lan, Wu, Ralph, John, and Coleman, Heather D. Sugarcane transgenics expressing MYB transcription factors show improved glucose release. United States: N. p., 2016. Web. doi:10.1186/s13068-016-0559-1.
Poovaiah, Charleson R., Bewg, William P., Lan, Wu, Ralph, John, & Coleman, Heather D. Sugarcane transgenics expressing MYB transcription factors show improved glucose release. United States. doi:10.1186/s13068-016-0559-1.
Poovaiah, Charleson R., Bewg, William P., Lan, Wu, Ralph, John, and Coleman, Heather D. Fri . "Sugarcane transgenics expressing MYB transcription factors show improved glucose release". United States. doi:10.1186/s13068-016-0559-1. https://www.osti.gov/servlets/purl/1299148.
@article{osti_1299148,
title = {Sugarcane transgenics expressing MYB transcription factors show improved glucose release},
author = {Poovaiah, Charleson R. and Bewg, William P. and Lan, Wu and Ralph, John and Coleman, Heather D.},
abstractNote = {In this study, sugarcane, a tropical C4 perennial crop, is capable of producing 30-100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and can provide a significant source of biomass for the production of second-generation bioethanol. As a result, MYB31 and MYB42 were cloned from maize and expressed in sugarcane with and without the UTR sequences. The cloned sequences were 98 and 99 % identical to the published nucleotide sequences. The inclusion of the UTR sequences did not affect any of the parameters tested. There was little difference in plant height and the number of internodes of the MYB-overexpressing sugarcane plants when compared with controls. MYB transgene expression determined by qPCR exhibited continued expression in young and maturing internodes. MYB31 downregulated more genes within the lignin biosynthetic pathway than MYB42. MYB31 and MYB42 expression resulted in decreased lignin content in some lines. All MYB42 plants further analyzed showed significant increases in glucose release by enzymatic hydrolysis in 72 h, whereas only two MYB31 plants released more glucose than control plants. This correlated directly with a significant decrease in acid-insoluble lignin. Soluble sucrose content of the MYB42 transgenic plants did not vary compared to control plants. In conclusion, this study demonstrates the use of MYB transcription factors to improve the production of bioethanol from sugarcane bagasse remaining after sugar extraction.},
doi = {10.1186/s13068-016-0559-1},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {2016},
month = {7}
}

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