Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass
Abstract
We report that cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture. Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations. Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses. Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]. This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform. In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied. For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes. The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that weremore »
- Authors:
-
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering; Rutgers Univ., Piscataway, NJ (United States). Dept. of Chemical and Biochemical Engineering
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1293995
- Grant/Contract Number:
- AC05-00OR22725; FC02-07ER64494; DBI-0421683; IOS-0923992
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Experimental Botany
- Additional Journal Information:
- Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; recalcitrance; plant biomass; glycome profiling; AFEX; biofuels; cell walls
Citation Formats
Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., and Chundawat, Shishir P. S.. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass. United States: N. p., 2015.
Web. doi:10.1093/jxb/erv107.
Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., & Chundawat, Shishir P. S.. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass. United States. https://doi.org/10.1093/jxb/erv107
Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., and Chundawat, Shishir P. S.. Thu .
"Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass". United States. https://doi.org/10.1093/jxb/erv107. https://www.osti.gov/servlets/purl/1293995.
@article{osti_1293995,
title = {Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass},
author = {Pattathil, Sivakumar and Hahn, Michael G. and Dale, Bruce E. and Chundawat, Shishir P. S.},
abstractNote = {We report that cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture. Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations. Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses. Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]. This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform. In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied. For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes. The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses. Lastly, we found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.},
doi = {10.1093/jxb/erv107},
journal = {Journal of Experimental Botany},
number = 14,
volume = 66,
place = {United States},
year = {2015},
month = {4}
}
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journal, August 2014
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journal, September 2009
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Covalent Cross-Links in the Cell Wall
journal, February 1994
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A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010
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Developmental and Tissue-Specific Structural Alterations of the Cell-Wall Polysaccharides of Arabidopsis thaliana Roots
journal, April 1996
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GUX1 and GUX2 glucuronyltransferases decorate distinct domains of glucuronoxylan with different substitution patterns
journal, March 2013
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- The Plant Journal, Vol. 74, Issue 3
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
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- Science, Vol. 315, Issue 5813, p. 804-807
How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012
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- Science, Vol. 338, Issue 6110
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011
- Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E.
- Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145
Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013
- Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
- Biotechnology for Biofuels, Vol. 6, Issue 1
Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material
journal, November 2014
- Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna
- Biotechnology for Biofuels, Vol. 7, Issue 1
Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance
journal, October 2014
- Chung, Daehwan; Pattathil, Sivakumar; Biswal, Ajaya K.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels
journal, November 2010
- Achyuthan, Komandoor Elayavalli; Achyuthan, Ann Mary; Adams, Paul David
- Molecules, Vol. 15, Issue 12
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment
text, January 2011
- Chundawat, Shishir P. S.; Donohoe, Bryon S.; Sousa, Leonardo
- Rutgers University
Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra)
text, January 2009
- Balan, Venkatesh; da Costa Sousa, Leonardo; Dale, Bruce E.
- Rutgers University
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Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells
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