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Title: Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass

Abstract

We report that cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture. Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations. Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses. Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]. This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform. In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied. For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes. The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that weremore » not detectable by conventional gross composition analyses. Lastly, we found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.« less

Authors:
 [1];  [1];  [2];  [3]
  1. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  2. Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering
  3. Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering; Rutgers Univ., Piscataway, NJ (United States). Dept. of Chemical and Biochemical Engineering
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1293995
Grant/Contract Number:  
AC05-00OR22725; FC02-07ER64494; DBI-0421683; IOS-0923992
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; recalcitrance; plant biomass; glycome profiling; AFEX; biofuels; cell walls

Citation Formats

Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., and Chundawat, Shishir P. S.. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass. United States: N. p., 2015. Web. doi:10.1093/jxb/erv107.
Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., & Chundawat, Shishir P. S.. Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass. United States. https://doi.org/10.1093/jxb/erv107
Pattathil, Sivakumar, Hahn, Michael G., Dale, Bruce E., and Chundawat, Shishir P. S.. Thu . "Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass". United States. https://doi.org/10.1093/jxb/erv107. https://www.osti.gov/servlets/purl/1293995.
@article{osti_1293995,
title = {Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass},
author = {Pattathil, Sivakumar and Hahn, Michael G. and Dale, Bruce E. and Chundawat, Shishir P. S.},
abstractNote = {We report that cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture. Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations. Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses. Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]. This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform. In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied. For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes. The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses. Lastly, we found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.},
doi = {10.1093/jxb/erv107},
journal = {Journal of Experimental Botany},
number = 14,
volume = 66,
place = {United States},
year = {Thu Apr 23 00:00:00 EDT 2015},
month = {Thu Apr 23 00:00:00 EDT 2015}
}

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Cited by: 42 works
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journal, June 2008


Revealing the structural and functional diversity of plant cell walls
journal, June 2008


Unique aspects of the grass cell wall
journal, June 2008


Pectin structure and biosynthesis
journal, June 2008


In Vitro Model Assemblies To Study the Impact of Lignin−Carbohydrate Interactions on the Enzymatic Conversion of Xylan
journal, September 2009

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Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate
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3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils
journal, August 2013

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journal, August 2014

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Covalent Cross-Links in the Cell Wall
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A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010

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Developmental and Tissue-Specific Structural Alterations of the Cell-Wall Polysaccharides of Arabidopsis thaliana Roots
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journal, March 2013

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journal, November 2012


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journal, July 2011


Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013

  • Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-71

Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material
journal, November 2014

  • Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna
  • Biotechnology for Biofuels, Vol. 7, Issue 1
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Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance
journal, October 2014

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Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels
journal, November 2010

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Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment
text, January 2011

  • Chundawat, Shishir P. S.; Donohoe, Bryon S.; Sousa, Leonardo
  • Rutgers University
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Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra)
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  • Rutgers University
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Works referencing / citing this record:

Probing the role of cation-π interaction in the thermotolerance and catalytic performance of endo-polygalacturonases
journal, December 2016

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Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling
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  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0703-6

Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover
journal, March 2017

  • Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar
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Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass
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Xylan epitope profiling: an enhanced approach to study organ development-dependent changes in xylan structure, biosynthesis, and deposition in plant cell walls
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  • DOI: 10.1038/srep38413

Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling
journal, February 2017

  • Walker, Johnnie A.; Pattathil, Sivakumar; Bergeman, Lai F.
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  • DOI: 10.1186/s13068-017-0703-6

Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover
journal, March 2017

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Xylan epitope profiling: an enhanced approach to study organ development-dependent changes in xylan structure, biosynthesis, and deposition in plant cell walls
journal, November 2017

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  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0935-5

Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells
journal, November 2017


Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery
journal, January 2018

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Comparative genome analysis indicates high evolutionary potential of pathogenicity genes in Colletotrichum tanaceti
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