High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling
Abstract
The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H2 and CO2 based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H2 with a yield of two H2 per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H2 productivity was increased 3-fold to 32 mmol H2∙L₋1∙h₋1. The productivity was further enhanced to 54 mmol H2∙L₋1∙h₋1 by increasing reaction temperature, substrate, and enzyme concentrations—an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.
- Authors:
-
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Cell-Free Bioinnovations, Blacksburg, VA (United States)
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Inst. for Critical Technology and Applied Science
- Cell-Free Bioinnovations, Blacksburg, VA (United States)
- Milwaukee School of Engineering, Milwaukee, WI (United States). Dept. of Physics and Chemistry
- Texas A&M Univ., Kingsville, TX (United States). Dept. of Chemical and Natural Gas Engineering
- Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
- Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Cell-Free Bioinnovations, Blacksburg, VA (United States); Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Inst. for Critical Technology and Applied Science; Chinese Academy of Sciences (CAS), Tianjin(China). Tianjin Inst. of Industrial Biotechnology
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1293980
- Grant/Contract Number:
- FG05-95ER20175; IIP-1321528; IIP-1353266
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 112; Journal Issue: 16; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; 09 BIOMASS FUELS; 08 HYDROGEN; hydrogen; biomass; in vitro metabolic engineering; metabolic network modeling; global sensitivity analysis
Citation Formats
Rollin, Joseph A., Martin del Campo, Julia, Myung, Suwan, Sun, Fangfang, You, Chun, Bakovic, Allison, Castro, Roberto, Chandrayan, Sanjeev K., Wu, Chang-Hao, Adams, Michael W. W., Senger, Ryan S., and Zhang, Y. -H. Percival. High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling. United States: N. p., 2015.
Web. doi:10.1073/pnas.1417719112.
Rollin, Joseph A., Martin del Campo, Julia, Myung, Suwan, Sun, Fangfang, You, Chun, Bakovic, Allison, Castro, Roberto, Chandrayan, Sanjeev K., Wu, Chang-Hao, Adams, Michael W. W., Senger, Ryan S., & Zhang, Y. -H. Percival. High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling. United States. https://doi.org/10.1073/pnas.1417719112
Rollin, Joseph A., Martin del Campo, Julia, Myung, Suwan, Sun, Fangfang, You, Chun, Bakovic, Allison, Castro, Roberto, Chandrayan, Sanjeev K., Wu, Chang-Hao, Adams, Michael W. W., Senger, Ryan S., and Zhang, Y. -H. Percival. Mon .
"High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling". United States. https://doi.org/10.1073/pnas.1417719112. https://www.osti.gov/servlets/purl/1293980.
@article{osti_1293980,
title = {High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling},
author = {Rollin, Joseph A. and Martin del Campo, Julia and Myung, Suwan and Sun, Fangfang and You, Chun and Bakovic, Allison and Castro, Roberto and Chandrayan, Sanjeev K. and Wu, Chang-Hao and Adams, Michael W. W. and Senger, Ryan S. and Zhang, Y. -H. Percival},
abstractNote = {The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H2 and CO2 based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H2 with a yield of two H2 per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H2 productivity was increased 3-fold to 32 mmol H2∙L₋1∙h₋1. The productivity was further enhanced to 54 mmol H2∙L₋1∙h₋1 by increasing reaction temperature, substrate, and enzyme concentrations—an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.},
doi = {10.1073/pnas.1417719112},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 16,
volume = 112,
place = {United States},
year = {Mon Apr 06 00:00:00 EDT 2015},
month = {Mon Apr 06 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Hydrogen Production Reactions from Carbon Feedstocks: Fossil Fuels and Biomass
journal, October 2007
- Navarro, R. M.; Peña, M. A.; Fierro, J. L. G.
- Chemical Reviews, Vol. 107, Issue 10
Rethinking biological activation of methane and conversion to liquid fuels
journal, April 2014
- Haynes, Chad A.; Gonzalez, Ramon
- Nature Chemical Biology, Vol. 10, Issue 5
Toward Efficient Hydrogen Production at Surfaces
journal, June 2006
- Norskov, J. K.; Christensen, C. H.
- Science, Vol. 312, Issue 5778
Nanoporous BiVO4 Photoanodes with Dual-Layer Oxygen Evolution Catalysts for Solar Water Splitting
journal, February 2014
- Kim, Tae Woo; Choi, Kyoung-Shin
- Science, Vol. 343, Issue 6174, p. 990-994
Hydrogen Production by Molecular Photocatalysis
journal, October 2007
- Esswein, Arthur J.; Nocera, Daniel G.
- Chemical Reviews, Vol. 107, Issue 10
The Hydrogen Issue
journal, December 2010
- Armaroli, Nicola; Balzani, Vincenzo
- ChemSusChem, Vol. 4, Issue 1
Engineering of yeast hexose transporters to transport D-xylose without inhibition by D-glucose
journal, March 2014
- Farwick, A.; Bruder, S.; Schadeweg, V.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 14
Supercritical water gasification of biomass: Thermodynamic constraints
journal, August 2011
- Castello, Daniele; Fiori, Luca
- Bioresource Technology, Vol. 102, Issue 16
Hydrogen production by recombinant Escherichia coli strains: H2 production by recombinant E.coli strains
journal, September 2011
- Maeda, Toshinari; Sanchez-Torres, Viviana; Wood, Thomas K.
- Microbial Biotechnology, Vol. 5, Issue 2
Hydrogen from catalytic reforming of biomass-derived hydrocarbons in liquid water
journal, August 2002
- Cortright, R. D.; Davda, R. R.; Dumesic, J. A.
- Nature, Vol. 418, Issue 6901
Biohydrogen as a renewable energy resource—Prospects and potentials
journal, January 2008
- Meherkotay, S.; Das, D.
- International Journal of Hydrogen Energy, Vol. 33, Issue 1
Biocommodity Engineering
journal, October 1999
- Lynd, L. R.; Wyman, C. E.; Gerngross, T. U.
- Biotechnology Progress, Vol. 15, Issue 5
A Functional Protein Chip for Pathway Optimization and in Vitro Metabolic Engineering
journal, April 2004
- Jung, G. Y.
- Science, Vol. 304, Issue 5669
New biotechnology paradigm: cell-free biosystems for biomanufacturing
journal, January 2013
- Rollin, Joseph A.; Tam, Tsz Kin; Zhang, Y. -H. Percival
- Green Chemistry, Vol. 15, Issue 7
The good of two worlds: increasing complexity in cell-free systems
journal, December 2013
- Billerbeck, Sonja; Härle, Johannes; Panke, Sven
- Current Opinion in Biotechnology, Vol. 24, Issue 6
Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry
journal, October 2012
- Harris, D. Calvin; Jewett, Michael C.
- Current Opinion in Biotechnology, Vol. 23, Issue 5
A synthetic biochemistry molecular purge valve module that maintains redox balance
journal, June 2014
- Opgenorth, Paul H.; Korman, Tyler P.; Bowie, James U.
- Nature Communications, Vol. 5, Issue 1
High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System
journal, March 2013
- Martín del Campo, Julia S.; Rollin, Joseph; Myung, Suwan
- Angewandte Chemie International Edition, Vol. 52, Issue 17
Cell-free synthetic biology: Thinking outside the cell
journal, May 2012
- Hodgman, C. Eric; Jewett, Michael C.
- Metabolic Engineering, Vol. 14, Issue 3
A high-energy-density sugar biobattery based on a synthetic enzymatic pathway
journal, January 2014
- Zhu, Zhiguang; Kin Tam, Tsz; Sun, Fangfang
- Nature Communications, Vol. 5, Issue 1
Enzymatic transformation of nonfood biomass to starch
journal, April 2013
- You, C.; Chen, H.; Myung, S.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 18
Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
journal, October 2012
- Guterl, Jan-Karl; Garbe, Daniel; Carsten, Jörg
- ChemSusChem, Vol. 5, Issue 11
Trends and innovations in industrial biocatalysis for the production of fine chemicals
journal, August 2004
- Panke, S.; Held, M.; Wubbolts, M.
- Current Opinion in Biotechnology, Vol. 15, Issue 4
Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes
journal, January 2011
- Tufvesson, Pär; Lima-Ramos, Joana; Nordblad, Mathias
- Organic Process Research & Development, Vol. 15, Issue 1
In silico evaluation of a complex multi-enzymatic system using one-pot and modular approaches: Application to the high-yield production of hydrogen from a synthetic metabolic pathway
journal, January 2013
- Ardao, Inés; Zeng, An-Ping
- Chemical Engineering Science, Vol. 87
Designing novel cellulase systems through agent-based modeling and global sensitivity analysis
journal, May 2014
- Apte, Advait A.; Senger, Ryan S.; Fong, Stephen S.
- Bioengineered, Vol. 5, Issue 4
Global sensitivity analysis of a process-based model for ammonia emissions from manure storage and treatment structures
journal, September 2010
- Ogejo, J. Arogo; Senger, R. S.; Zhang, R. H.
- Atmospheric Environment, Vol. 44, Issue 30
One-step purification and immobilization of thermophilic polyphosphate glucokinase from Thermobifida fusca YX: glucose-6-phosphate generation without ATP
journal, July 2011
- Liao, Hehuan; Myung, Suwan; Zhang, Y. -H. Percival
- Applied Microbiology and Biotechnology, Vol. 93, Issue 3
Comparative study of corn stover pretreated by dilute acid and cellulose solvent-based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility
journal, July 2009
- Zhu, Zhiguang; Sathitsuksanoh, Noppadon; Vinzant, Todd
- Biotechnology and Bioengineering, Vol. 103, Issue 4
Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails
journal, February 2009
- Ye, Xinhao; Wang, Yiran; Hopkins, Robert C.
- ChemSusChem, Vol. 2, Issue 2, p. 149-152
Is Simpler Better? Synthetic Nicotinamide Cofactor Analogues for Redox Chemistry
journal, January 2014
- Paul, Caroline E.; Arends, Isabel W. C. E.; Hollmann, Frank
- ACS Catalysis, Vol. 4, Issue 3
Bio-hydrogen production by different operational modes of dark and photo-fermentation: An overview
journal, July 2011
- Argun, Hidayet; Kargi, Fikret
- International Journal of Hydrogen Energy, Vol. 36, Issue 13
Editorial: The Nonsense of Biofuels
journal, February 2012
- Michel, Hartmut
- Angewandte Chemie International Edition, Vol. 51, Issue 11
Facilitated Substrate Channeling in a Self-Assembled Trifunctional Enzyme Complex
journal, July 2012
- You, Chun; Myung, Suwan; Zhang, Y. -H. Percival
- Angewandte Chemie International Edition, Vol. 51, Issue 35
Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation
journal, March 2014
- Brasen, C.; Esser, D.; Rauch, B.
- Microbiology and Molecular Biology Reviews, Vol. 78, Issue 1
Solar flux, water, and land impose limits on biology: Solar Flux, Water, and Land Impose Limits on Biology
journal, April 2014
- Jones, Mark E.; Banholzer, William F.
- Biotechnology and Bioengineering, Vol. 111, Issue 6
Biophysical Chemistry of Metabolic Reaction Sequences in Concentrated Enzyme Solution and in the Cell
journal, June 1987
- Srivastava, D. K.; Bernhard, S. A.
- Annual Review of Biophysics and Biophysical Chemistry, Vol. 16, Issue 1
Cell-Free Protein Expression under Macromolecular Crowding Conditions
journal, December 2011
- Ge, Xumeng; Luo, Dan; Xu, Jianfeng
- PLoS ONE, Vol. 6, Issue 12
Predicting in vivo drug interactions from in vitro drug discovery data
journal, October 2005
- Wienkers, Larry C.; Heath, Timothy G.
- Nature Reviews Drug Discovery, Vol. 4, Issue 10
Metabolite transfer via enzyme-enzyme complexes
journal, November 1986
- Srivastava, D.; Bernhard, S.
- Science, Vol. 234, Issue 4780
Engineering Hyperthermophilic Archaeon Pyrococcus furiosus to Overproduce Its Cytoplasmic [NiFe]-Hydrogenase
journal, December 2011
- Chandrayan, Sanjeev K.; McTernan, Patrick M.; Hopkins, R. Christopher
- Journal of Biological Chemistry, Vol. 287, Issue 5, p. 3257-3264
Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia
journal, September 2010
- Rollin, Joseph A.; Zhu, Zhiguang; Sathitsuksanoh, Noppadon
- Biotechnology and Bioengineering, Vol. 108, Issue 1
More Accurate Determination of Acid-Labile Carbohydrates in Lignocellulose by Modified Quantitative Saccharification
journal, November 2007
- Moxley, Geoffery; Zhang, Y. -H. Percival
- Energy & Fuels, Vol. 21, Issue 6
In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose
journal, July 2014
- Myung, Suwan; Rollin, Joseph; You, Chun
- Metabolic Engineering, Vol. 24
Facilitated Substrate Channeling in a Self-Assembled Trifunctional Enzyme Complex
journal, July 2012
- You, Chun; Myung, Suwan; Zhang, Y. -H. Percival
- Angewandte Chemie, Vol. 124, Issue 35
High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System
journal, March 2013
- Martín del Campo, Julia S.; Rollin, Joseph; Myung, Suwan
- Angewandte Chemie, Vol. 125, Issue 17
Hydrogen Production Reactions from Carbon Feedstocks: Fossil Fuels and Biomass
journal, December 2007
- Navarro, R. M.; Pena, M. A.; Fierro, J. L. G.
- ChemInform, Vol. 38, Issue 50
Hydrogen Production by Molecular Photocatalysis
journal, December 2007
- Esswein, Arthur J.; Nocera, Daviel G.
- ChemInform, Vol. 38, Issue 50
Works referencing / citing this record:
Macromolecular crowding effects on transcription and translation are regulated by free magnesium ion
journal, January 2020
- Ge, Xumeng; Xu, Jianfeng
- Biotechnology and Applied Biochemistry, Vol. 67, Issue 1
A Recombinant 12‐His Tagged Pyrococcus furiosus Soluble [NiFe]‐Hydrogenase I Overexpressed in Thermococcus kodakarensis KOD1 Facilitates Hydrogen‐Powered in vitro NADH Regeneration
journal, October 2018
- Song, Yunhong; Liu, Meixia; Xie, Leipeng
- Biotechnology Journal, Vol. 14, Issue 4
Permeabilized Escherichia coli Whole Cells Containing Co‐Expressed Two Thermophilic Enzymes Facilitate the Synthesis of scyllo ‐Inositol from myo ‐Inositol
journal, September 2019
- Li, Yuan; Liu, Shan; You, Chun
- Biotechnology Journal, Vol. 15, Issue 2
Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts
journal, June 2019
- Finnigan, William; Cutlan, Rhys; Snajdrova, Radka
- ChemCatChem, Vol. 11, Issue 15
Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces
journal, June 2018
- Yates, Nicholas D. J.; Fascione, Martin A.; Parkin, Alison
- Chemistry - A European Journal, Vol. 24, Issue 47
Evolutionary Prediction of Biohydrogen Production by Dark Fermentation
journal, November 2018
- Akhbari, Azam; Ibrahim, Shaliza; Zinatizadeh, Ali A.
- CLEAN - Soil, Air, Water, Vol. 47, Issue 1
Building a Thermostable Metabolon for Facilitating Coenzyme Transport and In Vitro Hydrogen Production at Elevated Temperature
journal, August 2018
- Chen, Hui; Huang, Rui; Kim, Eui-Jin
- ChemSusChem, Vol. 11, Issue 18
Catalytic Transfer Hydrogenation Using Biomass as Hydrogen Source
journal, August 2018
- Manna, Srimanta; Antonchick, Andrey P.
- ChemSusChem, Vol. 12, Issue 13
In vitro biosynthesis of optically pure d‐ (−)‐ acetoin from meso ‐2,3‐butanediol using 2,3‐butanediol dehydrogenase and NADH oxidase
journal, May 2019
- Cui, Zhenzhen; Zhao, Yujiao; Mao, Yufeng
- Journal of Chemical Technology & Biotechnology, Vol. 94, Issue 8
Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen production in vitro
journal, February 2018
- Huang, Rui; Chen, Hui; Zhou, Wei
- Applied Microbiology and Biotechnology, Vol. 102, Issue 7
Biomanufacturing: history and perspective
journal, November 2016
- Zhang, Yi-Heng Percival; Sun, Jibin; Ma, Yanhe
- Journal of Industrial Microbiology & Biotechnology, Vol. 44, Issue 4-5
A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo
journal, September 2019
- Li, Lu; Ye, Lei; Guo, Zhijie
- Biotechnology Letters, Vol. 41, Issue 11
In vitro multi-enzymatic cascades using recombinant lysates of E. coli: an emerging biocatalysis platform
journal, January 2020
- Alissandratos, Apostolos
- Biophysical Reviews, Vol. 12, Issue 1
Forward design of a complex enzyme cascade reaction
journal, September 2016
- Hold, Christoph; Billerbeck, Sonja; Panke, Sven
- Nature Communications, Vol. 7, Issue 1
Synthetic and systems biology for microbial production of commodity chemicals
journal, April 2016
- Chubukov, Victor; Mukhopadhyay, Aindrila; Petzold, Christopher J.
- npj Systems Biology and Applications, Vol. 2, Issue 1
Streamlined hydrogen production from biomass
journal, April 2018
- Zhang, Ping; Guo, Yan-Jun; Chen, Jianbin
- Nature Catalysis, Vol. 1, Issue 5
Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor
journal, November 2015
- Xie, Guo-Jun; Liu, Bing-Feng; Wang, Rui-Qing
- Scientific Reports, Vol. 5, Issue 1
Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae
journal, January 2016
- Zhang, Lijie; Liu, Qiuyuan; Ge, Yongsheng
- Green Chemistry, Vol. 18, Issue 6
Ultra-rapid rates of water splitting for biohydrogen gas production through in vitro artificial enzymatic pathways
journal, January 2018
- Kim, Eui-Jin; Kim, Jae-Eung; Zhang, Yi-Heng P. Job
- Energy & Environmental Science, Vol. 11, Issue 8
Electrical decoupling of microbial electrochemical reactions enables spontaneous H 2 evolution
journal, January 2020
- Chen, Xi; Lobo, Fernanda Leite; Bian, Yanhong
- Energy & Environmental Science, Vol. 13, Issue 2
Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production
journal, February 2018
- Wang, Yajie; Ren, Hengqian; Zhao, Huimin
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 53, Issue 2
Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX
journal, June 2018
- Zhou, Wei; Huang, Rui; Zhu, Zhiguang
- Applied and Environmental Microbiology, Vol. 84, Issue 16
Biosynthesis of Raffinose and Stachyose from Sucrose via an In Vitro Multienzyme System
journal, November 2018
- Tian, Chaoyu; Yang, Jiangang; Zeng, Yan
- Applied and Environmental Microbiology, Vol. 85, Issue 2
Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion
journal, April 2019
- Taniguchi, Hironori; Imura, Makoto; Okano, Kenji
- Microbial Cell Factories, Vol. 18, Issue 1
Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem
journal, November 2019
- Meng, Dongdong; Wu, Ranran; Wang, Juan
- Biotechnology for Biofuels, Vol. 12, Issue 1
Stoichiometric Regeneration of ATP by A NAD(P)/CoA-free and Phosphate-balanced In Vitro Synthetic Enzymatic Biosystem
journal, November 2018
- Wei, Xinlei; Xie, Leipeng; Job Zhang, Yi-Heng P.
- ChemCatChem, Vol. 10, Issue 24
Methanol-Water Aqueous-Phase Reforming with the Assistance of Dehydrogenases at Near-Room Temperature
journal, February 2018
- Shen, Yangbin; Zhan, Yulu; Li, Shuping
- ChemSusChem, Vol. 11, Issue 5
Hierarchical Micro‐ and Mesoporous Zn‐Based Metal–Organic Frameworks Templated by Hydrogels: Their Use for Enzyme Immobilization and Catalysis of Knoevenagel Reaction
journal, October 2019
- Cheng, Kaipeng; Svec, Frantisek; Lv, Yongqin
- Small, Vol. 15, Issue 49
In vitro production of cysteine from glucose
journal, August 2019
- Hanatani, Yohei; Imura, Makoto; Taniguchi, Hironori
- Applied Microbiology and Biotechnology, Vol. 103, Issue 19
Forward design of a complex enzyme cascade reaction
text, January 2016
- Christoph, Hold,; Sonja, Billerbeck,; Sven, Panke,
- ETH Zurich
Hierarchical Micro‐ and Mesoporous Zn‐Based Metal–Organic Frameworks Templated by Hydrogels: Their Use for Enzyme Immobilization and Catalysis of Knoevenagel Reaction
journal, September 2019
- Cheng, Kaipeng; Svec, Frantisek; Lv, Yongqin
- Small, Vol. 15, Issue 44
In vitro multi-enzymatic cascades using recombinant lysates of E. coli: an emerging biocatalysis platform
journal, January 2020
- Alissandratos, Apostolos
- Biophysical Reviews, Vol. 12, Issue 1
Protein engineering of oxidoreductases utilizing nicotinamide-based coenzymes, with applications in synthetic biology
journal, September 2017
- You, Chun; Huang, Rui; Wei, Xinlei
- Synthetic and Systems Biotechnology, Vol. 2, Issue 3
Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions
journal, September 2018
- Xie, Leipeng; Wei, Xinlei; Zhou, Xigui
- Synthetic and Systems Biotechnology, Vol. 3, Issue 3
Metabolite-based mutualism enhances hydrogen production in a two-species microbial consortium
journal, February 2019
- Wang, Shaojie; Tang, Hongzhi; Peng, Fei
- Communications Biology, Vol. 2, Issue 1
Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor
journal, November 2015
- Xie, Guo-Jun; Liu, Bing-Feng; Wang, Rui-Qing
- Scientific Reports, Vol. 5, Issue 1
Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus
journal, January 2015
- Wu, Chang-Hao; McTernan, Patrick M.; Walter, Mary E.
- Archaea, Vol. 2015
Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion
journal, April 2019
- Taniguchi, Hironori; Imura, Makoto; Okano, Kenji
- Microbial Cell Factories, Vol. 18, Issue 1
Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem
journal, November 2019
- Meng, Dongdong; Wu, Ranran; Wang, Juan
- Biotechnology for Biofuels, Vol. 12, Issue 1