Cell Wall Composition and Underlying QTL in an F1 Pseudo-Testcross Population of Switchgrass
Abstract
Natural genetic variation for reduced recalcitrance can be used to improve switchgrass for biofuel production. A full-sib switchgrass mapping population developed by crossing a lowland genotype, AP13, and upland genotype, VS16, was evaluated at three locations (Ardmore and Burneyville, OK and Watkinsville, GA). Biomass harvested after senescence in 2009 and 2010 was evaluated at the National Renewable Energy Laboratory (NREL) for sugar release using enzymatic hydrolysis and for lignin content and syringyl/guaiacyl lignin monomer (S/G) ratio using pyrolysis molecular beam mass spectrometry (py-MBMS). Glucose and xylose release ranged from 120 to 313 and 123 to 263 mg g-1, respectively, while lignin content ranged from 19 to 27% of the dry biomass. Statistically significant differences were observed among the genotypes and the environments for the cell wall composition traits. Regression analysis showed that a unit increase in lignin content reduced total sugar release by an average of 10 mg g-1. Quantitative trait loci (QTL) analysis detected 9 genomic regions underlying sugar release and 14 for lignin content. The phenotypic variation explained by the individual QTL identified for sugar release ranged from 4.5 to 9.4 and for lignin content from 3.8 to 11.1%. Mapping of the QTL regions to the switchgrass genomemore »
- Authors:
-
- Samuel Roberts Noble Foundation, Ardmore, OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Kansas State Univ., Manhattan, KS (United States). Agricultural Research Center-Hays
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Univ. of Georgia, Athens, GA (United States). Inst. of Plant Breeding, Genetics and Genomics, and Dept. of Crop and Soil Sciences; Univ. of Georgia, Athens, GA (United States). Dept. of Plant Biology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of California, Davis, CA (United States). Dept. of Plant Sciences
- Samuel Roberts Noble Foundation, Ardmore, OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1293804
- Report Number(s):
- NREL/JA-5100-66447
Journal ID: ISSN 1939-1234
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- BioEnergy Research
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 3; Journal ID: ISSN 1939-1234
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; lignin content; quantitative trait loci; sugar release; glucose; xylose; recalcitrant
Citation Formats
Serba, Desalegn D., Sykes, Robert W., Gjersing, Erica L., Decker, Stephen R., Daverdin, Guillaume, Devos, Katrien M., Brummer, E. Charles, and Saha, Malay C. Cell Wall Composition and Underlying QTL in an F1 Pseudo-Testcross Population of Switchgrass. United States: N. p., 2016.
Web. doi:10.1007/s12155-016-9733-3.
Serba, Desalegn D., Sykes, Robert W., Gjersing, Erica L., Decker, Stephen R., Daverdin, Guillaume, Devos, Katrien M., Brummer, E. Charles, & Saha, Malay C. Cell Wall Composition and Underlying QTL in an F1 Pseudo-Testcross Population of Switchgrass. United States. https://doi.org/10.1007/s12155-016-9733-3
Serba, Desalegn D., Sykes, Robert W., Gjersing, Erica L., Decker, Stephen R., Daverdin, Guillaume, Devos, Katrien M., Brummer, E. Charles, and Saha, Malay C. Sat .
"Cell Wall Composition and Underlying QTL in an F1 Pseudo-Testcross Population of Switchgrass". United States. https://doi.org/10.1007/s12155-016-9733-3. https://www.osti.gov/servlets/purl/1293804.
@article{osti_1293804,
title = {Cell Wall Composition and Underlying QTL in an F1 Pseudo-Testcross Population of Switchgrass},
author = {Serba, Desalegn D. and Sykes, Robert W. and Gjersing, Erica L. and Decker, Stephen R. and Daverdin, Guillaume and Devos, Katrien M. and Brummer, E. Charles and Saha, Malay C.},
abstractNote = {Natural genetic variation for reduced recalcitrance can be used to improve switchgrass for biofuel production. A full-sib switchgrass mapping population developed by crossing a lowland genotype, AP13, and upland genotype, VS16, was evaluated at three locations (Ardmore and Burneyville, OK and Watkinsville, GA). Biomass harvested after senescence in 2009 and 2010 was evaluated at the National Renewable Energy Laboratory (NREL) for sugar release using enzymatic hydrolysis and for lignin content and syringyl/guaiacyl lignin monomer (S/G) ratio using pyrolysis molecular beam mass spectrometry (py-MBMS). Glucose and xylose release ranged from 120 to 313 and 123 to 263 mg g-1, respectively, while lignin content ranged from 19 to 27% of the dry biomass. Statistically significant differences were observed among the genotypes and the environments for the cell wall composition traits. Regression analysis showed that a unit increase in lignin content reduced total sugar release by an average of 10 mg g-1. Quantitative trait loci (QTL) analysis detected 9 genomic regions underlying sugar release and 14 for lignin content. The phenotypic variation explained by the individual QTL identified for sugar release ranged from 4.5 to 9.4 and for lignin content from 3.8 to 11.1%. Mapping of the QTL regions to the switchgrass genome sequence (v1.1) found that some of the QTL colocalized with genes involved in carbohydrate processing and metabolism, plant development, defense systems, and transcription factors. Finally, the markers associated with QTL can be implemented in breeding programs to efficiently develop improved switchgrass cultivars for biofuel production.},
doi = {10.1007/s12155-016-9733-3},
journal = {BioEnergy Research},
number = 3,
volume = 9,
place = {United States},
year = {Sat Apr 23 00:00:00 EDT 2016},
month = {Sat Apr 23 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 11 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
QTL for Yield, Earliness, and Cell Wall Quality Traits in Topcross Experiments of the F838 × F286 Early Maize RIL Progeny
journal, September 2010
- Barrière, Yves; Méchin, Valérie; Denoue, Dominique
- Crop Science, Vol. 50, Issue 5
Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment
journal, January 2010
- Li, Xu; Ximenes, Eduardo; Kim, Youngmi
- Biotechnology for Biofuels, Vol. 3, Issue 1
Expression of an Arabidopsis phosphoglycerate mutase homologue is localized to apical meristems, regulated by hormones, and induced by sedentary plant-parasitic nematodes
journal, November 2003
- Mazarei, Mitra; Lennon, Kristen A.; Puthoff, David P.
- Plant Molecular Biology, Vol. 53, Issue 4
Chemical Composition of Parenchyma and Sclerenchyma Cell Walls Isolated from Orchardgrass and Switchgrass
journal, January 1991
- Grabber, J. H.; Jung, G. A.; Hill, R. R.
- Crop Science, Vol. 31, Issue 4
Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment
journal, January 2010
- Li, Xu; Ximenes, Eduardo; Kim, Youngmi
- Biotechnology for Biofuels, Vol. 3, Issue 1
QTL for Yield, Earliness, and Cell Wall Quality Traits in Topcross Experiments of the F838 × F286 Early Maize RIL Progeny
journal, September 2010
- Barrière, Yves; Méchin, Valérie; Denoue, Dominique
- Crop Science, Vol. 50, Issue 5
High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy
book, January 2009
- Sykes, Robert; Yung, Matthew; Novaes, Evandro
- Biofuels
Switchgrass Breeding, Genetics, and Genomics
book, January 2012
- Casler, Michael D.
- Green Energy and Technology (book series)
Lignin: Characterization of a Multifaceted Crop Component
journal, January 2013
- Frei, Michael
- The Scientific World Journal, Vol. 2013
ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010
- Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
- Industrial Biotechnology, Vol. 6, Issue 2
Mapping QTLs with epistatic effects and QTL × treatment interactions for salt tolerance at seedling stage of wheat
journal, February 2012
- Xu, Yun-Feng; An, Diao-Guo; Liu, Dong-Cheng
- Euphytica, Vol. 186, Issue 1
Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011
- Xu, Bin; Escamilla-Treviño, Luis L.; Sathitsuksanoh, Noppadon
- New Phytologist, Vol. 192, Issue 3
The glycolytic enzyme, phosphoglycerate mutase, has critical roles in stomatal movement, vegetative growth, and pollen production in Arabidopsis thaliana
journal, August 2011
- Zhao, Zhixin; Assmann, Sarah M.
- Journal of Experimental Botany, Vol. 62, Issue 14
The MYB Transcription Factor Superfamily of Arabidopsis: Expression Analysis and Phylogenetic Comparison with the Rice MYB Family
journal, January 2006
- Yanhui, Chen; Xiaoyuan, Yang; Kun, He
- Plant Molecular Biology, Vol. 60, Issue 1
Genetic Modification of Herbaceous Plants for Feed and Fuel
journal, January 2001
- Vogel, Kenneth P.; Jung, Hans-Joachim G.
- Critical Reviews in Plant Sciences, Vol. 20, Issue 1
The glycolytic enzyme, phosphoglycerate mutase, has critical roles in stomatal movement, vegetative growth, and pollen production in Arabidopsis thaliana
journal, August 2011
- Zhao, Zhixin; Assmann, Sarah M.
- Journal of Experimental Botany, Vol. 62, Issue 14
Rapid Assessment of Lignin Content and Structure in Switchgrass (Panicum virgatum L.) Grown Under Different Environmental Conditions
journal, October 2009
- Mann, David G. J.; Labbé, Nicole; Sykes, Robert W.
- BioEnergy Research, Vol. 2, Issue 4
High resolution of quantitative traits into multiple loci via interval mapping.
journal, April 1994
- Jansen, R. C.; Stam, P.
- Genetics, Vol. 136, Issue 4
Lignification of switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii) plant parts during maturation and its effect on fibre degradability
journal, January 1992
- Jung, Hans-Joachim G.; Vogel, Kenneth P.
- Journal of the Science of Food and Agriculture, Vol. 59, Issue 2
A Genomics Approach to Deciphering Lignin Biosynthesis in Switchgrass
journal, November 2013
- Shen, H.; Mazarei, M.; Hisano, H.
- The Plant Cell, Vol. 25, Issue 11
Linkage Maps of Lowland and Upland Tetraploid Switchgrass Ecotypes
journal, March 2013
- Serba, Desalegn; Wu, Limin; Daverdin, Guillaume
- BioEnergy Research, Vol. 6, Issue 3
Variation of S/G Ratio and Lignin Content in a <i>Populus</i> Family Influences the Release of Xylose by Dilute Acid Hydrolysis
journal, January 2006
- Davison, Brian H.; Drescher, Sadie R.; Tuskan, Gerald A.
- Applied Biochemistry and Biotechnology, Vol. 130, Issue 1-3
High-Throughput Screening Techniques for Biomass Conversion
journal, October 2009
- Decker, Stephen R.; Brunecky, Roman; Tucker, Melvin P.
- BioEnergy Research, Vol. 2, Issue 4
Switchgrass Breeding, Genetics, and Genomics
book, January 2012
- Casler, Michael D.
- Green Energy and Technology (book series)
Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production: Overexpression of miR156 improved switchgrass biomass
journal, January 2012
- Fu, Chunxiang; Sunkar, Ramanjulu; Zhou, Chuanen
- Plant Biotechnology Journal, Vol. 10, Issue 4
Carbon Source-Dependent Phosphorylation of Hexokinase PII and Its Role in the Glucose-Signaling Response in Yeast
journal, May 1998
- Randez-Gil, Francisca; Sanz, Pascual; Entian, Karl-Dieter
- Molecular and Cellular Biology, Vol. 18, Issue 5
The Roles of Auxin Response Factor Domains in Auxin-Responsive Transcription
journal, January 2003
- Tiwari, Shiv B.; Hagen, Gretchen; Guilfoyle, Tom
- The Plant Cell, Vol. 15, Issue 2
Ethanol yields and cell wall properties in divergently bred switchgrass genotypes
journal, October 2011
- Sarath, Gautam; Dien, Bruce; Saathoff, Aaron J.
- Bioresource Technology, Vol. 102, Issue 20
Comparing the Recalcitrance of Eucalyptus, Pine, and Switchgrass Using Ionic Liquid and Dilute Acid Pretreatments
journal, May 2012
- Li, Chenlin; Sun, Lan; Simmons, Blake A.
- BioEnergy Research, Vol. 6, Issue 1
Lignin content in natural Populus variants affects sugar release
journal, March 2011
- Studer, M. H.; DeMartini, J. D.; Davis, M. F.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011
- Shen, Hui; He, Xianzhi; Poovaiah, Charleson R.
- New Phytologist, Vol. 193, Issue 1, p. 121-136
Identification of quantitative trait loci and epistatic interactions for plant height and heading date in rice
journal, March 2002
- Yu, S. B.; Li, J. X.; Xu, C. G.
- Theoretical and Applied Genetics, Vol. 104, Issue 4
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
- Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
Estimation of Genetic Parameters for Biomass Yield in Lowland Switchgrass (Panicum virgatum L.)
journal, January 2011
- Bhandari, H. S.; Saha, M. C.; Fasoula, V. A.
- Crop Science, Vol. 51, Issue 4
Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass
journal, October 2006
- Dien, B.; Jung, H.; Vogel, K.
- Biomass and Bioenergy, Vol. 30, Issue 10, p. 880-891
Variation among Half-Sib Families and Heritability for Biomass Yield and Other Traits in Lowland Switchgrass ( L.)
journal, January 2010
- Bhandari, H. S.; Saha, M. C.; Mascia, P. N.
- Crop Science, Vol. 50, Issue 6
Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States
journal, June 2005
- McLaughlin, Samuel B.; Adams Kszos, Lynn
- Biomass and Bioenergy, Vol. 28, Issue 6
Maximum Likelihood Techniques for the Mapping and Analysis of Quantitative Trait Loci with the Aid of Genetic Markers
journal, September 1986
- Weller, J. I.
- Biometrics, Vol. 42, Issue 3
Molecular breeding of switchgrass for use as a biofuel crop
journal, December 2007
- Bouton, Joseph H.
- Current Opinion in Genetics & Development, Vol. 17, Issue 6
Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations
journal, November 2007
- Li, Huihui; Ribaut, Jean-Marcel; Li, Zhonglai
- Theoretical and Applied Genetics, Vol. 116, Issue 2
Efficient Method for Analysis of QTL Using F1 Progenies in an Outcrossing Species
journal, October 2004
- Hayashi, Takeshi; Awata, Takashi
- Genetica, Vol. 122, Issue 2
Quantitative Trait Loci (QTL) Underlying Biomass Yield and Plant Height in Switchgrass
journal, August 2014
- Serba, Desalegn D.; Daverdin, Guillaume; Bouton, Joseph H.
- BioEnergy Research, Vol. 8, Issue 1
Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013
- Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
- Biotechnology for Biofuels, Vol. 6, Issue 1
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
QTL mapping for lignin content, lignin monomeric composition, p-hydroxycinnamate content, and cell wall digestibility in the maize recombinant inbred line progeny F838×F286
journal, October 2008
- Barrière, Y.; Thomas, J.; Denoue, D.
- Plant Science, Vol. 175, Issue 4
QTL analysis of main and epistatic effects for flour color traits in durum wheat
journal, February 2012
- Roncallo, Pablo F.; Cervigni, Gerardo L.; Jensen, Carlos
- Euphytica, Vol. 185, Issue 1
Ethanol yields and cell wall properties in divergently bred switchgrass genotypes
journal, October 2011
- Sarath, Gautam; Dien, Bruce; Saathoff, Aaron J.
- Bioresource Technology, Vol. 102, Issue 20
Polymorphisms in monolignol biosynthetic genes are associated with biomass yield and agronomic traits in European maize (Zea mays L.)
journal, January 2010
- Chen, Yongsheng; Zein, Imad; Brenner, Everton
- BMC Plant Biology, Vol. 10, Issue 1
QTL mapping for lignin content, lignin monomeric composition, p-hydroxycinnamate content, and cell wall digestibility in the maize recombinant inbred line progeny F838×F286
journal, October 2008
- Barrière, Y.; Thomas, J.; Denoue, D.
- Plant Science, Vol. 175, Issue 4
Rewriting the lignin roadmap
journal, June 2002
- Humphreys, John M.; Chapple, Clint
- Current Opinion in Plant Biology, Vol. 5, Issue 3
Estimation of genetic variability and heritability for biofuel feedstock yield in several populations of switchgrass
journal, February 2008
- Rose, L. W.; Das, M. K.; Taliaferro, C. M.
- Annals of Applied Biology, Vol. 152, Issue 1
Mapping QTLs with epistatic effects and QTL × environment interactions for plant height using a doubled haploid population in cultivated wheat
journal, February 2008
- Zhang, Kunpu; Tian, Jichun; Zhao, Liang
- Journal of Genetics and Genomics, Vol. 35, Issue 2
Genetic structure composed of additive QTL, epistatic QTL pairs and collective unmapped minor QTL conferring oil content and fatty acid components of soybeans
journal, September 2011
- Li, Haiwang; Zhao, Tuanjie; Wang, Yufeng
- Euphytica, Vol. 182, Issue 1
Mapping QTLs with epistatic effect and QTL×environmental interaction effect of heading date in rice: Mapping QTLs with epistatic effect and QTL×environmental interaction effect of heading date in rice
journal, June 2010
- Lei, Dong-yang; Chen, Li-yun
- JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY, Vol. 36, Issue 3
Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013
- Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
- Biotechnology for Biofuels, Vol. 6, Issue 1
Quantitative trait loci for seed yield and yield-related traits, and their responses to reduced phosphorus supply in Brassica napus
journal, January 2012
- Ding, Guangda; Zhao, Zunkang; Liao, Yuan
- Annals of Botany, Vol. 109, Issue 4
Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release
journal, February 2010
- Studer, Michael H.; DeMartini, Jaclyn D.; Brethauer, Simone
- Biotechnology and Bioengineering, Vol. 105, Issue 2, p. 231-238
Quantitative Trait Loci (QTL) Underlying Biomass Yield and Plant Height in Switchgrass
journal, August 2014
- Serba, Desalegn D.; Daverdin, Guillaume; Bouton, Joseph H.
- BioEnergy Research, Vol. 8, Issue 1
Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States
journal, June 2005
- McLaughlin, Samuel B.; Adams Kszos, Lynn
- Biomass and Bioenergy, Vol. 28, Issue 6
Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment
journal, July 2015
- Scullin, Chessa; Cruz, Alejandro G.; Chuang, Yi-De
- Biotechnology for Biofuels, Vol. 8, Issue 1
Mapping mendelian factors underlying quantitative traits using RFLP linkage maps.
journal, January 1989
- Lander, E. S.; Botstein, D.
- Genetics, Vol. 121, Issue 1
High resolution of quantitative traits into multiple loci via interval mapping.
journal, April 1994
- Jansen, R. C.; Stam, P.
- Genetics, Vol. 136, Issue 4
Works referencing / citing this record:
Transcriptome divergence during leaf development in two contrasting switchgrass (Panicum virgatum L.) cultivars
journal, September 2019
- Palmer, Nathan A.; Chowda-Reddy, R. V.; Muhle, Anthony A.
- PLOS ONE, Vol. 14, Issue 9
Development and field assessment of transgenic hybrid switchgrass for improved biofuel traits
journal, January 2020
- Alexander, Lisa; Hatcher, Catherine; Mazarei, Mitra
- Euphytica, Vol. 216, Issue 2
Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition
journal, May 2017
- Belmokhtar, Nassim; Arnoult, Stéphanie; Chabbert, Brigitte
- Frontiers in Plant Science, Vol. 8
High‐density linkage map reveals QTL underlying growth traits in AP13×VS16 biparental population of switchgrass
journal, January 2019
- Ali, Shahjahan; Serba, Desalegn D.; Jenkins, Jerry
- GCB Bioenergy, Vol. 11, Issue 5