A Genetic Screen Identifies a Requirement for Cysteine-Rich–Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity
Abstract
Systemic acquired resistance, mediated by the Arabidopsis NPR1 gene and the rice NH1 gene, confers broad-spectrum immunity to diverse pathogens. NPR1 and NH1 interact with TGA transcription factors to activate downstream defense genes. Despite the importance of this defense response, the signaling components downstream of NPR1/NH1 and TGA proteins are poorly defined. Here we report the identification of a rice mutant, snim1, which suppresses NH1-mediated immunity and demonstrate that two genes encoding previously uncharacterized cysteine-rich-receptor-like kinases (CRK6 and CRK10), complement the snim1 mutant phenotype. Silencing of CRK6 and CRK10 genes individually in the parental genetic background recreates the snim1 phenotype. We identified a rice mutant in the Kitaake genetic background with a frameshift mutation in crk10; this mutant also displays a compromised immune response highlighting the important role of crk10. We also show that elevated levels of NH1 expression lead to enhanced CRK10 expression and that the rice TGA2.1 protein binds to the CRK10 promoter. Furthermore, these experiments demonstrate a requirement for CRKs in NH1-mediated immunity and establish a molecular link between NH1 and induction of CRK10 expression.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of California, Davis, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1338422
- Alternate Identifier(s):
- OSTI ID: 1288267
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- PLoS Genetics
- Additional Journal Information:
- Journal Name: PLoS Genetics Journal Volume: 12 Journal Issue: 5; Journal ID: ISSN 1553-7404
- Publisher:
- Public Library of Science (PLoS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; systemic acquired-resistance; enhanced disease resistance; lesion mimic mutants; salicylic-acid; transcription factors; arabidopsis npr1; pseudomonas-syringae; negative regulator; xanthomonas-oryzae; btb/poz domain
Citation Formats
Chern, Mawsheng, Xu, Qiufang, Bart, Rebecca S., Bai, Wei, Ruan, Deling, Sze-To, Wing Hoi, Canlas, Patrick E., Jain, Rashmi, Chen, Xuewei, Ronald, Pamela C., and Wang, ed., Guo-Liang. A Genetic Screen Identifies a Requirement for Cysteine-Rich–Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity. United States: N. p., 2016.
Web. doi:10.1371/journal.pgen.1006049.
Chern, Mawsheng, Xu, Qiufang, Bart, Rebecca S., Bai, Wei, Ruan, Deling, Sze-To, Wing Hoi, Canlas, Patrick E., Jain, Rashmi, Chen, Xuewei, Ronald, Pamela C., & Wang, ed., Guo-Liang. A Genetic Screen Identifies a Requirement for Cysteine-Rich–Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity. United States. https://doi.org/10.1371/journal.pgen.1006049
Chern, Mawsheng, Xu, Qiufang, Bart, Rebecca S., Bai, Wei, Ruan, Deling, Sze-To, Wing Hoi, Canlas, Patrick E., Jain, Rashmi, Chen, Xuewei, Ronald, Pamela C., and Wang, ed., Guo-Liang. Fri .
"A Genetic Screen Identifies a Requirement for Cysteine-Rich–Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity". United States. https://doi.org/10.1371/journal.pgen.1006049.
@article{osti_1338422,
title = {A Genetic Screen Identifies a Requirement for Cysteine-Rich–Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity},
author = {Chern, Mawsheng and Xu, Qiufang and Bart, Rebecca S. and Bai, Wei and Ruan, Deling and Sze-To, Wing Hoi and Canlas, Patrick E. and Jain, Rashmi and Chen, Xuewei and Ronald, Pamela C. and Wang, ed., Guo-Liang},
abstractNote = {Systemic acquired resistance, mediated by the Arabidopsis NPR1 gene and the rice NH1 gene, confers broad-spectrum immunity to diverse pathogens. NPR1 and NH1 interact with TGA transcription factors to activate downstream defense genes. Despite the importance of this defense response, the signaling components downstream of NPR1/NH1 and TGA proteins are poorly defined. Here we report the identification of a rice mutant, snim1, which suppresses NH1-mediated immunity and demonstrate that two genes encoding previously uncharacterized cysteine-rich-receptor-like kinases (CRK6 and CRK10), complement the snim1 mutant phenotype. Silencing of CRK6 and CRK10 genes individually in the parental genetic background recreates the snim1 phenotype. We identified a rice mutant in the Kitaake genetic background with a frameshift mutation in crk10; this mutant also displays a compromised immune response highlighting the important role of crk10. We also show that elevated levels of NH1 expression lead to enhanced CRK10 expression and that the rice TGA2.1 protein binds to the CRK10 promoter. Furthermore, these experiments demonstrate a requirement for CRKs in NH1-mediated immunity and establish a molecular link between NH1 and induction of CRK10 expression.},
doi = {10.1371/journal.pgen.1006049},
journal = {PLoS Genetics},
number = 5,
volume = 12,
place = {United States},
year = {Fri May 13 00:00:00 EDT 2016},
month = {Fri May 13 00:00:00 EDT 2016}
}
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