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Title: Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii

Upon fertilization Chlamydomonas reinhardtii zygotes undergo a program of differentiation into a diploid zygospore that is accompanied by transcription of hundreds of zygote-specific genes. We identified a distinct sequence motif we term a zygotic response element (ZYRE) that is highly enriched in promoter regions of C. reinhardtii early zygotic genes. A luciferase reporter assay was used to show that native ZYRE motifs within the promoter of zygotic gene ZYS3 or intron of zygotic gene DMT4 are necessary for zygotic induction. A synthetic luciferase reporter with a minimal promoter was used to show that ZYRE motifs introduced upstream are sufficient to confer zygotic upregulation, and that ZYRE-controlled zygotic transcription is dependent on the homeodomain transcription factor GSP1. Furthermore, we predict that ZYRE motifs will correspond to binding sites for the homeodomain proteins GSP1-GSM1 that heterodimerize and activate zygotic gene expression in early zygotes.
Authors:
 [1] ;  [2] ;  [2] ;  [1]
  1. Donald Danforth Plant Science Center, St Louis, MO (United States)
  2. Univ. of California, Los Angeles, Los Angeles, CA (United States). Dept. of Molecular, Cell, and Developmental Biology
Publication Date:
Grant/Contract Number:
FC02-02ER63421
Type:
Accepted Manuscript
Journal Name:
G3
Additional Journal Information:
Journal Volume: 6; Journal Issue: 6; Journal ID: ISSN 2160-1836
Publisher:
Genetics Society of America
Research Org:
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Chlamydomonas; cis-regulatory element; fertilization; homeodomain protein; zygote; mating-type locus; chloroplast dna; gamete fusion; genome; transcription; inheritance; evolution; promoter; reporter
OSTI Identifier:
1287328

Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, and Umen, James. Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii. United States: N. p., Web. doi:10.1534/g3.116.029181.
Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, & Umen, James. Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii. United States. doi:10.1534/g3.116.029181.
Hamaji, Takashi, Lopez, David, Pellegrini, Matteo, and Umen, James. 2016. "Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii". United States. doi:10.1534/g3.116.029181. https://www.osti.gov/servlets/purl/1287328.
@article{osti_1287328,
title = {Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii},
author = {Hamaji, Takashi and Lopez, David and Pellegrini, Matteo and Umen, James},
abstractNote = {Upon fertilization Chlamydomonas reinhardtii zygotes undergo a program of differentiation into a diploid zygospore that is accompanied by transcription of hundreds of zygote-specific genes. We identified a distinct sequence motif we term a zygotic response element (ZYRE) that is highly enriched in promoter regions of C. reinhardtii early zygotic genes. A luciferase reporter assay was used to show that native ZYRE motifs within the promoter of zygotic gene ZYS3 or intron of zygotic gene DMT4 are necessary for zygotic induction. A synthetic luciferase reporter with a minimal promoter was used to show that ZYRE motifs introduced upstream are sufficient to confer zygotic upregulation, and that ZYRE-controlled zygotic transcription is dependent on the homeodomain transcription factor GSP1. Furthermore, we predict that ZYRE motifs will correspond to binding sites for the homeodomain proteins GSP1-GSM1 that heterodimerize and activate zygotic gene expression in early zygotes.},
doi = {10.1534/g3.116.029181},
journal = {G3},
number = 6,
volume = 6,
place = {United States},
year = {2016},
month = {3}
}