Short-Time Glassy Dynamics in Viscous Protein Solutions with Competing Interactions
Abstract
Although there have been numerous investigations of the glass transition for colloidal dispersions with only a short-ranged attraction, less is understood for systems interacting with a long-ranged repulsion in addition to this attraction, which is ubiquitous in aqueous protein solutions at low ionic strength. Highly puri ed concentrated lysozyme solutions are used as a model system and investigated over a large range of protein concentrations at very low ionic strength. Newtonian liquid behavior is observed at all concentrations, even up to 480 mg/mL, where the zero shear viscosity increases by more than three orders of magnitude with increasing concentration. Remarkably, despite this macroscopic liquid-like behavior, the measurements of the dynamics in the short-time limit shows features typical of glassy colloidal systems. Investigation of the inter-protein structure indicates that the reduced short-time mobility of the protein is caused by localized regions of high density within a heterogeneous density distribution. This structural heterogeneity occurs on intermediate range length scale, driven by the competing potential features, and is distinct from commonly studied colloidal gel systems in which a heterogeneous density distribution tends to extend to the whole system. The presence of long-ranged repulsion also allows for more mobility over large length and longmore »
- Authors:
-
- Univ. of Delaware, Newark, DE (United States)
- National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Inst. Laue-Langevin (ILL), Grenoble (France)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1286934
- Alternate Identifier(s):
- OSTI ID: 1227033
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Physical Review Letters
- Additional Journal Information:
- Journal Volume: 115; Journal Issue: 22; Journal ID: ISSN 0031-9007
- Publisher:
- American Physical Society (APS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Godfrin, P. Douglas, Hudson, Steven, Hong, Kunlun, Porcar, Lionel, Falus, Peter, Wagner, Norman, and Liu, Yun. Short-Time Glassy Dynamics in Viscous Protein Solutions with Competing Interactions. United States: N. p., 2015.
Web. doi:10.1103/PhysRevLett.115.228302.
Godfrin, P. Douglas, Hudson, Steven, Hong, Kunlun, Porcar, Lionel, Falus, Peter, Wagner, Norman, & Liu, Yun. Short-Time Glassy Dynamics in Viscous Protein Solutions with Competing Interactions. United States. https://doi.org/10.1103/PhysRevLett.115.228302
Godfrin, P. Douglas, Hudson, Steven, Hong, Kunlun, Porcar, Lionel, Falus, Peter, Wagner, Norman, and Liu, Yun. Tue .
"Short-Time Glassy Dynamics in Viscous Protein Solutions with Competing Interactions". United States. https://doi.org/10.1103/PhysRevLett.115.228302. https://www.osti.gov/servlets/purl/1286934.
@article{osti_1286934,
title = {Short-Time Glassy Dynamics in Viscous Protein Solutions with Competing Interactions},
author = {Godfrin, P. Douglas and Hudson, Steven and Hong, Kunlun and Porcar, Lionel and Falus, Peter and Wagner, Norman and Liu, Yun},
abstractNote = {Although there have been numerous investigations of the glass transition for colloidal dispersions with only a short-ranged attraction, less is understood for systems interacting with a long-ranged repulsion in addition to this attraction, which is ubiquitous in aqueous protein solutions at low ionic strength. Highly puri ed concentrated lysozyme solutions are used as a model system and investigated over a large range of protein concentrations at very low ionic strength. Newtonian liquid behavior is observed at all concentrations, even up to 480 mg/mL, where the zero shear viscosity increases by more than three orders of magnitude with increasing concentration. Remarkably, despite this macroscopic liquid-like behavior, the measurements of the dynamics in the short-time limit shows features typical of glassy colloidal systems. Investigation of the inter-protein structure indicates that the reduced short-time mobility of the protein is caused by localized regions of high density within a heterogeneous density distribution. This structural heterogeneity occurs on intermediate range length scale, driven by the competing potential features, and is distinct from commonly studied colloidal gel systems in which a heterogeneous density distribution tends to extend to the whole system. The presence of long-ranged repulsion also allows for more mobility over large length and long time scales resulting in the macroscopic relaxation of the structure. The experimental results provide evidence for the need to explicitly include intermediate range order in theories for the macroscopic properties of protein solutions interacting via competing potential features.},
doi = {10.1103/PhysRevLett.115.228302},
journal = {Physical Review Letters},
number = 22,
volume = 115,
place = {United States},
year = {Tue Nov 24 00:00:00 EST 2015},
month = {Tue Nov 24 00:00:00 EST 2015}
}
Web of Science
Works referenced in this record:
A Microliter Capillary Rheometer for Characterization of Protein Solutions
journal, February 2015
- Hudson, Steven D.; Sarangapani, Prasad; Pathak, Jai A.
- Journal of Pharmaceutical Sciences, Vol. 104, Issue 2
Cluster-Driven Dynamical Arrest in Concentrated Lysozyme Solutions
journal, June 2011
- Cardinaux, Frédéric; Zaccarelli, Emanuela; Stradner, Anna
- The Journal of Physical Chemistry B, Vol. 115, Issue 22
Short-time transport properties in dense suspensions: From neutral to charge-stabilized colloidal spheres
journal, March 2008
- Banchio, Adolfo J.; Nägele, Gerhard
- The Journal of Chemical Physics, Vol. 128, Issue 10
Protein self-diffusion in crowded solutions
journal, July 2011
- Roosen-Runge, F.; Hennig, M.; Zhang, F.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 29
Protein hydration dynamics in solution: a critical survey
journal, August 2004
- Halle, Bertil
- Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 359, Issue 1448
On the importance of thermodynamic self-consistency for calculating clusterlike pair correlations in hard-core double Yukawa fluids
journal, February 2011
- Kim, Jung Min; Castañeda-Priego, Ramón; Liu, Yun
- The Journal of Chemical Physics, Vol. 134, Issue 6
Structural and Dynamical Features of Multiple Metastable Glassy States in a Colloidal System with Competing Interactions
journal, April 2010
- Klix, Christian L.; Royall, C. Patrick; Tanaka, Hajime
- Physical Review Letters, Vol. 104, Issue 16
Observation of Small Cluster Formation in Concentrated Monoclonal Antibody Solutions and Its Implications to Solution Viscosity
journal, April 2014
- Yearley, Eric J.; Godfrin, Paul D.; Perevozchikova, Tatiana
- Biophysical Journal, Vol. 106, Issue 8
Compressibility of globular proteins in water at 25.degree.C
journal, October 1979
- Gekko, Kunihiko.; Noguchi, Hajime.
- The Journal of Physical Chemistry, Vol. 83, Issue 21
Incorporation of partially purified hen egg white lysozyme into zein films for antimicrobial food packaging
journal, January 2006
- Mecitoğlu, Çiğdem; Yemenicioğlu, Ahmet; Arslanoğlu, Alper
- Food Research International, Vol. 39, Issue 1
Lysozyme Protein Solution with an Intermediate Range Order Structure
journal, June 2011
- Liu, Yun; Porcar, Lionel; Chen, Jinhong
- The Journal of Physical Chemistry B, Vol. 115, Issue 22
Cluster formation in two-Yukawa fluids
journal, January 2005
- Liu, Yun; Chen, Wei-Ren; Chen, Sow-Hsin
- The Journal of Chemical Physics, Vol. 122, Issue 4
Brownian motion, hydrodynamics, and the osmotic pressure
journal, February 1993
- Brady, John F.
- The Journal of Chemical Physics, Vol. 98, Issue 4
Equilibrium Cluster Phases and Low-Density Arrested Disordered States: The Role of Short-Range Attraction and Long-Range Repulsion
journal, July 2004
- Sciortino, Francesco; Mossa, Stefano; Zaccarelli, Emanuela
- Physical Review Letters, Vol. 93, Issue 5
Scaling of Dynamics with the Range of Interaction in Short-Range Attractive Colloids
journal, February 2005
- Foffi, G.; Michele, C. De; Sciortino, F.
- Physical Review Letters, Vol. 94, Issue 7
Divergence in the low shear viscosity for Brownian hard-sphere dispersions: At random close packing or the glass transition?
journal, November 2013
- Russel, William B.; Wagner, Norman J.; Mewis, Jan
- Journal of Rheology, Vol. 57, Issue 6
Microscopic Diffusion and Hydrodynamic Interactions of Hemoglobin in Red Blood Cells
journal, August 2007
- Doster, Wolfgang; Longeville, Stéphane
- Biophysical Journal, Vol. 93, Issue 4
Colloidal systems with competing interactions: from an arrested repulsive cluster phase to a gel
journal, January 2009
- Toledano, Juan Carlos Fernandez; Sciortino, Francesco; Zaccarelli, Emanuela
- Soft Matter, Vol. 5, Issue 12
Equilibrium cluster formation in concentrated protein solutions and colloids
journal, November 2004
- Stradner, Anna; Sedgwick, Helen; Cardinaux, Frédéric
- Nature, Vol. 432, Issue 7016
Development of Filler Structure in Colloidal Silica–Polymer Nanocomposites
journal, October 2011
- Meth, Jeffrey S.; Zane, Stephen G.; Chi, Changzai
- Macromolecules, Vol. 44, Issue 20
Formation of the Dynamic Clusters in Concentrated Lysozyme Protein Solutions
journal, November 2009
- Porcar, Lionel; Falus, Peter; Chen, Wei-Ren
- The Journal of Physical Chemistry Letters, Vol. 1, Issue 1
Solubility of Lysozyme in Polyethylene Glycol-Electrolyte Mixtures: The Depletion Interaction and Ion-Specific Effects
journal, August 2008
- Bončina, Matjaž; Reščič, Jurij; Vlachy, Vojko
- Biophysical Journal, Vol. 95, Issue 3
Model colloidal fluid with competing interactions: Bulk and interfacial properties
journal, January 2007
- Archer, A. J.; Pini, D.; Evans, R.
- The Journal of Chemical Physics, Vol. 126, Issue 1
One liquid, two glasses
journal, November 2002
- Sciortino, Francesco
- Nature Materials, Vol. 1, Issue 3
Rheology of globular proteins: apparent yield stress, high shear rate viscosity and interfacial viscoelasticity of bovine serum albumin solutions
journal, January 2011
- Sharma, Vivek; Jaishankar, Aditya; Wang, Ying-Chih
- Soft Matter, Vol. 7, Issue 11
Collective diffusion, self-diffusion and freezing criteria of colloidal suspensions
journal, August 2000
- Banchio, Adolfo J.; Nägele, Gerhard; Bergenholtz, Johan
- The Journal of Chemical Physics, Vol. 113, Issue 8
Dynamical Arrest Transition in Nanoparticle Dispersions with Short-Range Interactions
journal, March 2011
- Eberle, Aaron P. R.; Wagner, Norman J.; Castañeda-Priego, Ramón
- Physical Review Letters, Vol. 106, Issue 10
Direct observation of a local structural mechanism for dynamic arrest
journal, June 2008
- Patrick Royall, C.; Williams, Stephen R.; Ohtsuka, Takehiro
- Nature Materials, Vol. 7, Issue 7
Percolation in colloidal systems with competing interactions: the role of long-range repulsion
journal, January 2013
- Valadez-Pérez, Néstor E.; Castañeda-Priego, Ramón; Liu, Yun
- RSC Advances, Vol. 3, Issue 47
Model for Reversible Colloidal Gelation
journal, June 2005
- Zaccarelli, E.; Buldyrev, S. V.; La Nave, E.
- Physical Review Letters, Vol. 94, Issue 21
Colloidal gels: equilibrium and non-equilibrium routes
journal, July 2007
- Zaccarelli, Emanuela
- Journal of Physics: Condensed Matter, Vol. 19, Issue 32
Dynamical Arrest in Attractive Colloids: The Effect of Long-Range Repulsion
journal, May 2005
- Campbell, Andrew I.; Anderson, Valerie J.; van Duijneveldt, Jeroen S.
- Physical Review Letters, Vol. 94, Issue 20
Competing Interactions in Arrested States of Colloidal Clays
journal, February 2010
- Ruzicka, B.; Zulian, L.; Zaccarelli, E.
- Physical Review Letters, Vol. 104, Issue 8
Mechanisms and Dynamics of Protein Clustering on a Solid Surface
journal, February 2008
- Mulheran, P. A.; Pellenc, D.; Bennett, R. A.
- Physical Review Letters, Vol. 100, Issue 6
Phase Behavior of Small Attractive Colloidal Particles
journal, January 1996
- Rosenbaum, D.; Zamora, P. C.; Zukoski, C. F.
- Physical Review Letters, Vol. 76, Issue 1, p. 150-153
Interplay between Spinodal Decomposition and Glass Formation in Proteins Exhibiting Short-Range Attractions
journal, September 2007
- Cardinaux, Frédéric; Gibaud, Thomas; Stradner, Anna
- Physical Review Letters, Vol. 99, Issue 11
Dynamical arrest in adhesive hard-sphere dispersions driven by rigidity percolation
journal, December 2013
- Valadez-Pérez, Néstor E.; Liu, Yun; Eberle, Aaron P. R.
- Physical Review E, Vol. 88, Issue 6
Modeling the electrophoresis of rigid polyions: application to lysozyme
journal, June 1995
- Allison, S. A.; Tran, V. T.
- Biophysical Journal, Vol. 68, Issue 6
Measurement of the self-intermediate scattering function of suspensions of hard spherical particles near the glass transition
journal, November 1998
- van Megen, W.; Mortensen, T. C.; Williams, S. R.
- Physical Review E, Vol. 58, Issue 5
Rheology and Dynamics of Colloidal Suspensions
journal, February 1999
- Banchio, A. J.; Bergenholtz, J.; Nägele, G.
- Physical Review Letters, Vol. 82, Issue 8
Linear Viscoelasticity of Colloidal Hard Sphere Suspensions near the Glass Transition
journal, October 1995
- Mason, T. G.; Weitz, D. A.
- Physical Review Letters, Vol. 75, Issue 14
Colloidal Gels: Equilibrium and Non-Equilibrium Routes
text, January 2007
- Zaccarelli, Emanuela
- arXiv
Competing interactions in arrested states of colloidal clays
text, January 2010
- Ruzicka, B.; Zulian, L.; Zaccarelli, E.
- arXiv
Equilibrium cluster phases and low-density arrested disordered states: The role of short-range attraction and long-range repulsion
text, January 2003
- Sciortino, Francesco; Mossa, Stefano; Zaccarelli, Emanuela
- arXiv
Model for reversible colloidal gelation
text, January 2004
- Zaccarelli, E.; Buldyrev, S. V.; La Nave, E.
- arXiv
Dynamical Arrest in Attractive Colloids: The Effect of Long-Range Repulsion
text, January 2004
- Campbell, Andrew I.; Anderson, Valerie J.; van Duijneveldt, Jeroen S.
- arXiv
Works referencing / citing this record:
Short-time dynamics of lysozyme solutions with competing short-range attraction and long-range repulsion: Experiment and theory
journal, February 2018
- Riest, Jonas; Nägele, Gerhard; Liu, Yun
- The Journal of Chemical Physics, Vol. 148, Issue 6
Multi-attribute PAT for UF/DF of Proteins—Monitoring Concentration, particle sizes, and Buffer Exchange
journal, February 2020
- Rolinger, Laura; Rüdt, Matthias; Diehm, Juliane
- Analytical and Bioanalytical Chemistry, Vol. 412, Issue 9
Investigating the effective interaction between silica colloidal particles near the critical point of a binary solvent by small angle neutron scattering
journal, August 2018
- Wang, Zhiyuan; Guo, Hongyu; Liu, Yun
- The Journal of Chemical Physics, Vol. 149, Issue 8
Dynamic cluster formation determines viscosity and diffusion in dense protein solutions
journal, April 2019
- von Bülow, Sören; Siggel, Marc; Linke, Max
- Proceedings of the National Academy of Sciences, Vol. 116, Issue 20
Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation
journal, February 2019
- Marioli, Maria; Kok, Wim Th.
- Analytical and Bioanalytical Chemistry, Vol. 411, Issue 11
Dynamics of proteins in solution
journal, January 2019
- Grimaldo, Marco; Roosen-Runge, Felix; Zhang, Fajun
- Quarterly Reviews of Biophysics, Vol. 52
Microliter viscometry using a bright-field microscope: η -DDM
journal, January 2018
- Escobedo-Sánchez, M. A.; Segovia-Gutiérrez, J. P.; Zuccolotto-Bernez, A. B.
- Soft Matter, Vol. 14, Issue 34
Tween protects recombinant human growth hormone against agitation-induced damage via hydrophobic interactions
journal, December 1998
- Bam, Narendra B.; Cleland, Jeffrey L.; Yang, Janet
- Journal of Pharmaceutical Sciences, Vol. 87, Issue 12