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Title: Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution

Abstract

Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Furthermore, using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution.

Authors:
 [1];  [2];  [1]
  1. Ames Lab. and Iowa State Univ., Ames, IA (United States)
  2. Ames Lab., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Lab., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1278749
Report Number(s):
IS-J-8999
Journal ID: ISSN 1044-0305; PII: 1422
Grant/Contract Number:  
AC02-07CH11358
Resource Type:
Accepted Manuscript
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Name: Journal of the American Society for Mass Spectrometry; Journal ID: ISSN 1044-0305
Publisher:
American Society for Mass Spectrometry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; mass spectrometry imaging; MALDI; matrix; recrystallization; maize; lipids; high spatial resolution

Citation Formats

Duenas, Maria Emilia, Carlucci, Laura, and Lee, Young Jin. Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution. United States: N. p., 2016. Web. https://doi.org/10.1007/s13361-016-1422-0.
Duenas, Maria Emilia, Carlucci, Laura, & Lee, Young Jin. Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution. United States. https://doi.org/10.1007/s13361-016-1422-0
Duenas, Maria Emilia, Carlucci, Laura, and Lee, Young Jin. Mon . "Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution". United States. https://doi.org/10.1007/s13361-016-1422-0. https://www.osti.gov/servlets/purl/1278749.
@article{osti_1278749,
title = {Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution},
author = {Duenas, Maria Emilia and Carlucci, Laura and Lee, Young Jin},
abstractNote = {Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Furthermore, using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution.},
doi = {10.1007/s13361-016-1422-0},
journal = {Journal of the American Society for Mass Spectrometry},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {6}
}

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