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Title: Matrix recrystallization for MALDI-MS imaging of maize lipids at high-spatial resolution

Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Furthermore, using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution.
Authors:
 [1] ;  [2] ;  [1]
  1. Ames Lab. and Iowa State Univ., Ames, IA (United States)
  2. Ames Lab., Ames, IA (United States)
Publication Date:
Report Number(s):
IS-J--8999
Journal ID: ISSN 1044-0305; PII: 1422
Grant/Contract Number:
AC02-07CH11358
Type:
Accepted Manuscript
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Name: Journal of the American Society for Mass Spectrometry; Journal ID: ISSN 1044-0305
Publisher:
American Society for Mass Spectrometry
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY mass spectrometry imaging; MALDI; matrix; recrystallization; maize; lipids; high spatial resolution
OSTI Identifier:
1278749
Alternate Identifier(s):
OSTI ID: 1278749