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Title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Abstract

Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. Lastly, it was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Louisiana Tech Univ., Ruston, LA (United States)
Publication Date:
Research Org.:
Louisiana Tech Univ., Ruston, LA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1272638
Alternate Identifier(s):
OSTI ID: 1242890
Grant/Contract Number:  
EE0003120
Resource Type:
Published Article
Journal Name:
SpringerPlus
Additional Journal Information:
Journal Volume: 5; Journal Issue: 1; Journal ID: ISSN 2193-1801
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cellulase; (3-Aminopropyl) triethoxy-silane; glutaraldehyde; glucose; silica gel

Citation Formats

Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Snow, L. Dale, and Palmer, James. Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer. United States: N. p., 2016. Web. doi:10.1186/s40064-016-1682-y.
Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Snow, L. Dale, & Palmer, James. Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer. United States. doi:10.1186/s40064-016-1682-y.
Zhang, Dezhi, Hegab, Hisham E., Lvov, Yuri, Snow, L. Dale, and Palmer, James. Wed . "Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer". United States. doi:10.1186/s40064-016-1682-y.
@article{osti_1272638,
title = {Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer},
author = {Zhang, Dezhi and Hegab, Hisham E. and Lvov, Yuri and Snow, L. Dale and Palmer, James},
abstractNote = {Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. Lastly, it was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.},
doi = {10.1186/s40064-016-1682-y},
journal = {SpringerPlus},
number = 1,
volume = 5,
place = {United States},
year = {2016},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s40064-016-1682-y

Citation Metrics:
Cited by: 2 works
Citation information provided by
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