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Title: A transgenic approach for controlling Lygus in cotton

Abstract

Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [3];  [1];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Monsanto Company, Chesterfield, MO (United States)
  2. Monsanto Company, Chesterfield, MO (United States); Visterra Inc, Cambridge, MA (United States)
  3. Cornell Univ., Ithaca, NY (United States); Monsanto Company, Chesterfield, MO (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1267458
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Agricultural genetics; Mutagenesis; Transgenic plants

Citation Formats

Gowda, Anilkumar, Rydel, Timothy J., Wollacott, Andrew M., Brown, Robert S., Akbar, Waseem, Clark, Thomas L., Flasinski, Stanislaw, Nageotte, Jeffrey R., Read, Andrew C., Shi, Xiaohong, Werner, Brent J., Pleau, Michael J., and Baum, James A. A transgenic approach for controlling Lygus in cotton. United States: N. p., 2016. Web. doi:10.1038/ncomms12213.
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Gowda, Anilkumar, Rydel, Timothy J., Wollacott, Andrew M., Brown, Robert S., Akbar, Waseem, Clark, Thomas L., Flasinski, Stanislaw, Nageotte, Jeffrey R., Read, Andrew C., Shi, Xiaohong, Werner, Brent J., Pleau, Michael J., & Baum, James A. A transgenic approach for controlling Lygus in cotton. United States. https://doi.org/10.1038/ncomms12213
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Gowda, Anilkumar, Rydel, Timothy J., Wollacott, Andrew M., Brown, Robert S., Akbar, Waseem, Clark, Thomas L., Flasinski, Stanislaw, Nageotte, Jeffrey R., Read, Andrew C., Shi, Xiaohong, Werner, Brent J., Pleau, Michael J., and Baum, James A. Mon . "A transgenic approach for controlling Lygus in cotton". United States. https://doi.org/10.1038/ncomms12213. https://www.osti.gov/servlets/purl/1267458.
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@article{osti_1267458,
title = {A transgenic approach for controlling Lygus in cotton},
author = {Gowda, Anilkumar and Rydel, Timothy J. and Wollacott, Andrew M. and Brown, Robert S. and Akbar, Waseem and Clark, Thomas L. and Flasinski, Stanislaw and Nageotte, Jeffrey R. and Read, Andrew C. and Shi, Xiaohong and Werner, Brent J. and Pleau, Michael J. and Baum, James A.},
abstractNote = {Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.},
doi = {10.1038/ncomms12213},
journal = {Nature Communications},
number = 1,
volume = 7,
place = {United States},
year = {Mon Jul 18 00:00:00 EDT 2016},
month = {Mon Jul 18 00:00:00 EDT 2016}
}
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https://doi.org/10.1038/ncomms12213
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Works referenced in this record:

Modification of the coding sequence enhances plant expression of insect control protein genes.
journal, April 1991

  • Perlak, F. J.; Fuchs, R. L.; Dean, D. A.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 8
  • DOI: 10.1073/pnas.88.8.3324

Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin from Bacillus thuringiensis
journal, July 2015

  • Xu, Chengchen; Chinte, Unmesh; Chen, Lirong
  • Biochemical and Biophysical Research Communications, Vol. 462, Issue 3
  • DOI: 10.1016/j.bbrc.2015.04.068

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

Optimal docking area: A new method for predicting protein-protein interaction sites
journal, October 2004

  • Fernandez-Recio, Juan; Totrov, Max; Skorodumov, Constantin
  • Proteins: Structure, Function, and Bioinformatics, Vol. 58, Issue 1
  • DOI: 10.1002/prot.20285

Functional Significance of Loops in The Receptor Binding Domain ofBacillus thuringiensisCryIIIA δ-Endotoxin
journal, February 1996

Lepidopteran-Active Variable-Region Sequence Imparts Coleopteran Activity in eCry3.1Ab, an Engineered Bacillus thuringiensis Hybrid Insecticidal Protein
journal, March 2010

  • Walters, F. S.; deFontes, C. M.; Hart, H.
  • Applied and Environmental Microbiology, Vol. 76, Issue 10
  • DOI: 10.1128/AEM.00155-10

Unusual Amino Acid Determinants of Host Range in the Mtx2 Family of Mosquitocidal Toxins
journal, June 1996

  • Chan, Siew Wee; Thanabalu, Thirumaran; Wee, Boon Yu
  • Journal of Biological Chemistry, Vol. 271, Issue 24
  • DOI: 10.1074/jbc.271.24.14183

Maximum-likelihood density modification
journal, August 2000

  • Terwilliger, Thomas C.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 8
  • DOI: 10.1107/S0907444900005072

Acephate Resistance in Populations of the Tarnished Plant Bug (Heteroptera: Miridae) From the Mississippi River Delta
journal, April 2009

  • Snodgrass, G. L.; Gore, J.; Abel, C. A.
  • Journal of Economic Entomology, Vol. 102, Issue 2
  • DOI: 10.1603/029.102.0231

Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells
journal, February 2009

  • Akiba, Toshihiko; Abe, Yuichi; Kitada, Sakae
  • Journal of Molecular Biology, Vol. 386, Issue 1
  • DOI: 10.1016/j.jmb.2008.12.002

Tarnished Plant Bug (Hemiptera: Miridae) Thresholds and Sampling Comparisons for Flowering Cotton in the Midsouthern United States
journal, October 2009

  • Musser, Fred R.; Catchot, Angus L.; Stewart, Scott D.
  • Journal of Economic Entomology, Vol. 102, Issue 5
  • DOI: 10.1603/029.102.0513

Suppression of Cotton Bollworm in Multiple Crops in China in Areas with Bt Toxin-Containing Cotton
journal, September 2008

Improved methods for building protein models in electron density maps and the location of errors in these models
journal, March 1991

  • Jones, T. A.; Zou, J. Y.; Cowan, S. W.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119
  • DOI: 10.1107/S0108767390010224

Clostridium perfringens ε-toxin shows structural similarity to the pore-forming toxin aerolysin
journal, July 2004

  • Cole, Ambrose R.; Gibert, Maryse; Popoff, Michel
  • Nature Structural & Molecular Biology, Vol. 11, Issue 8
  • DOI: 10.1038/nsmb804

Protein engineering of Bacillus thuringiensis  -endotoxin: Mutations at domain II of CryIAb enhance receptor affinity and toxicity toward gypsy moth larvae
journal, December 1996

  • Rajamohan, F.; Alzate, O.; Cotrill, J. A.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 25
  • DOI: 10.1073/pnas.93.25.14338

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

Mirid Bug Outbreaks in Multiple Crops Correlated with Wide-Scale Adoption of Bt Cotton in China
journal, May 2010

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

A Method of Controlling Corn Rootworm Feeding Using a Bacillus thuringiensis Protein Expressed in Transgenic Maize
journal, May 2005

Prediction-based protein engineering of domain I of Cry2A entomocidal toxin of Bacillus thuringiensis for the enhancement of toxicity against lepidopteran insects
journal, November 2007

  • Mandal, Chandi C.; Gayen, Srimonta; Basu, Asitava
  • Protein Engineering, Design and Selection, Vol. 20, Issue 12
  • DOI: 10.1093/protein/gzm058

Optimal docking area: A new method for predicting protein-protein interaction sites
journal, October 2004

  • Fernandez-Recio, Juan; Totrov, Max; Skorodumov, Constantin
  • Proteins: Structure, Function, and Bioinformatics, Vol. 58, Issue 1
  • DOI: 10.1002/prot.20285

Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin from Bacillus thuringiensis
journal, July 2015

  • Xu, Chengchen; Chinte, Unmesh; Chen, Lirong
  • Biochemical and Biophysical Research Communications, Vol. 462, Issue 3
  • DOI: 10.1016/j.bbrc.2015.04.068

Clostridium perfringens ε-toxin shows structural similarity to the pore-forming toxin aerolysin
journal, July 2004

  • Cole, Ambrose R.; Gibert, Maryse; Popoff, Michel
  • Nature Structural & Molecular Biology, Vol. 11, Issue 8
  • DOI: 10.1038/nsmb804

Modification of the coding sequence enhances plant expression of insect control protein genes.
journal, April 1991

  • Perlak, F. J.; Fuchs, R. L.; Dean, D. A.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 8
  • DOI: 10.1073/pnas.88.8.3324

Protein engineering of Bacillus thuringiensis  -endotoxin: Mutations at domain II of CryIAb enhance receptor affinity and toxicity toward gypsy moth larvae
journal, December 1996

  • Rajamohan, F.; Alzate, O.; Cotrill, J. A.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 25
  • DOI: 10.1073/pnas.93.25.14338

Unusual Amino Acid Determinants of Host Range in the Mtx2 Family of Mosquitocidal Toxins
journal, June 1996

  • Chan, Siew Wee; Thanabalu, Thirumaran; Wee, Boon Yu
  • Journal of Biological Chemistry, Vol. 271, Issue 24
  • DOI: 10.1074/jbc.271.24.14183

Prediction-based protein engineering of domain I of Cry2A entomocidal toxin of Bacillus thuringiensis for the enhancement of toxicity against lepidopteran insects
journal, November 2007

  • Mandal, Chandi C.; Gayen, Srimonta; Basu, Asitava
  • Protein Engineering, Design and Selection, Vol. 20, Issue 12
  • DOI: 10.1093/protein/gzm058

Automated MAD and MIR structure solution
journal, April 1999

  • Terwilliger, Thomas C.; Berendzen, Joel
  • Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 4
  • DOI: 10.1107/s0907444999000839

Suppression of Cotton Bollworm in Multiple Crops in China in Areas with Bt Toxin-Containing Cotton
journal, September 2008

Mirid Bug Outbreaks in Multiple Crops Correlated with Wide-Scale Adoption of Bt Cotton in China
journal, May 2010

Participation of Valine 171 in  -Helix 5 of Bacillus thuringiensis Cry1Ab  -Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes
journal, October 2010

  • Alzate, O.; Osorio, C.; Florez, A. M.
  • Applied and Environmental Microbiology, Vol. 76, Issue 23
  • DOI: 10.1128/aem.01428-10

An Engineered Chymotrypsin/Cathepsin G Site in Domain I Renders Bacillus thuringiensis Cry3A Active against Western Corn Rootworm Larvae
journal, November 2007

  • Walters, F. S.; Stacy, C. M.; Lee, M. K.
  • Applied and Environmental Microbiology, Vol. 74, Issue 2
  • DOI: 10.1128/aem.02165-07

Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins
journal, January 1998

Acephate Resistance in Populations of the Tarnished Plant Bug (Heteroptera: Miridae) From the Mississippi River Delta
journal, April 2009

  • Snodgrass, G. L.; Gore, J.; Abel, C. A.
  • Journal of Economic Entomology, Vol. 102, Issue 2
  • DOI: 10.1603/029.102.0231

Cotton Plants Expressing a Hemipteran-Active Bacillus thuringiensis Crystal Protein Impact the Development and Survival of Lygus hesperus (Hemiptera: Miridae) Nymphs
journal, April 2012

  • Baum, James A.; Sukuru, Uma R.; Penn, Stephen R.
  • Journal of Economic Entomology, Vol. 105, Issue 2
  • DOI: 10.1603/ec11207
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    First transgenic trait for control of plant bugs and thrips in cotton: Bt cotton controls plant bugs and thrips
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    journal, November 2019

    • Huseth, Anders S.; D′Ambrosio, Damon A.; Yorke, Benjamin T.
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    Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests
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    Behavioral Responses of Thrips (Thysanoptera: Thripidae) and Tarnished Plant Bug (Hemiptera: Miridae) to a New Bt Toxin, Cry51Aa2.834_16 in Cotton
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    Behavioral Responses of Thrips (Thysanoptera: Thripidae) and Tarnished Plant Bug (Hemiptera: Miridae) to a New Bt Toxin, Cry51Aa2.834_16 in Cotton
    journal, March 2019

    • Graham, Scott H.; Musser, Fred M.; Jacobson, Alana L.
    • Journal of Economic Entomology, Vol. 112, Issue 4
    • DOI: 10.1093/jee/toz058
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