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Title: Effects of Nanoparticle Morphology and Acyl Chain Length on Spontaneous Lipid Transfer Rates

Abstract

In this paper, we report on studies of lipid transfer rates between different morphology nanoparticles and lipids with different length acyl chains. The lipid transfer rate of dimyristoylphosphatidylcholine (di-C14, DMPC) in discoidal “bicelles” (0.156 h–1) is 2 orders of magnitude greater than that of DMPC vesicles (ULVs) (1.1 × 10–3 h–1). For both bicellar and ULV morphologies, increasing the acyl chain length by two carbons [going from di-C14 DMPC to di-C16, dipalmitoylphosphatidylcholine (DPPC)] causes lipid transfer rates to decrease by more than 2 orders of magnitude. Results from small angle neutron scattering (SANS), differential scanning calorimetry (DSC), and fluorescence correlation spectroscopy (FCS) are in good agreement. Finally, the present studies highlight the importance of lipid dynamic processes taking place in different morphology biomimetic membranes.

Authors:
 [1];  [2];  [1];  [1];  [3];  [4];  [5];  [6];  [7]
  1. Univ. of Connecticut, Storrs, CT (United States). Dept. of Chemical and Biomolecular Engineering
  2. Univ. of Connecticut, Storrs, CT (United States). Inst. of Materials Science. Polymer Program
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Neutron Sciences
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics and Astronomy; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Neutron Sciences
  5. Univ. of Notre Dame, IN (United States). Dept. of Chemical and Biomolecular Engineering
  6. Univ. of Notre Dame, IN (United States). Dept. of Chemical and Biomolecular Engineering; Wayne State Univ., Detroit, MI (United States). Dept. of Chemical Engineering and Materials Science
  7. Univ. of Connecticut, Storrs, CT (United States). Dept. of Chemical and Biomolecular Engineering; Univ. of Connecticut, Storrs, CT (United States). Inst. of Materials Science. Polymer Program; Univ. of Connecticut, Storrs, CT (United States). Dept. of Biomedical Engineering
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). National Science Foundation (NSF) (United States)
Contributing Org.:
Univ. of Connecticut, Storrs, CT (United States); Univ. of Notre Dame, IN (United States)
OSTI Identifier:
1265607
Grant/Contract Number:  
AC05-00OR22725; FG02-07ER46390; NSF-CMMI 1131587; CBET 1433903
Resource Type:
Accepted Manuscript
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 31; Journal Issue: 47; Journal ID: ISSN 0743-7463
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Xia, Yan, Li, Ming, Charubin, Kamil, Liu, Ying, Heberle, Frederick A., Katsaras, John, Jing, Benxin, Zhu, Yingxi, and Nieh, Mu-Ping. Effects of Nanoparticle Morphology and Acyl Chain Length on Spontaneous Lipid Transfer Rates. United States: N. p., 2015. Web. doi:10.1021/acs.langmuir.5b03291.
Xia, Yan, Li, Ming, Charubin, Kamil, Liu, Ying, Heberle, Frederick A., Katsaras, John, Jing, Benxin, Zhu, Yingxi, & Nieh, Mu-Ping. Effects of Nanoparticle Morphology and Acyl Chain Length on Spontaneous Lipid Transfer Rates. United States. https://doi.org/10.1021/acs.langmuir.5b03291
Xia, Yan, Li, Ming, Charubin, Kamil, Liu, Ying, Heberle, Frederick A., Katsaras, John, Jing, Benxin, Zhu, Yingxi, and Nieh, Mu-Ping. Thu . "Effects of Nanoparticle Morphology and Acyl Chain Length on Spontaneous Lipid Transfer Rates". United States. https://doi.org/10.1021/acs.langmuir.5b03291. https://www.osti.gov/servlets/purl/1265607.
@article{osti_1265607,
title = {Effects of Nanoparticle Morphology and Acyl Chain Length on Spontaneous Lipid Transfer Rates},
author = {Xia, Yan and Li, Ming and Charubin, Kamil and Liu, Ying and Heberle, Frederick A. and Katsaras, John and Jing, Benxin and Zhu, Yingxi and Nieh, Mu-Ping},
abstractNote = {In this paper, we report on studies of lipid transfer rates between different morphology nanoparticles and lipids with different length acyl chains. The lipid transfer rate of dimyristoylphosphatidylcholine (di-C14, DMPC) in discoidal “bicelles” (0.156 h–1) is 2 orders of magnitude greater than that of DMPC vesicles (ULVs) (1.1 × 10–3 h–1). For both bicellar and ULV morphologies, increasing the acyl chain length by two carbons [going from di-C14 DMPC to di-C16, dipalmitoylphosphatidylcholine (DPPC)] causes lipid transfer rates to decrease by more than 2 orders of magnitude. Results from small angle neutron scattering (SANS), differential scanning calorimetry (DSC), and fluorescence correlation spectroscopy (FCS) are in good agreement. Finally, the present studies highlight the importance of lipid dynamic processes taking place in different morphology biomimetic membranes.},
doi = {10.1021/acs.langmuir.5b03291},
journal = {Langmuir},
number = 47,
volume = 31,
place = {United States},
year = {2015},
month = {11}
}

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