skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source

Abstract

Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for fermentation-based bioprocesses are obtained from agricultural commodities that are used as foodstuff for human beings. Lignocellulose-derived sugars as the non-food, green, and sustainable alternative carbon sources have great potential to avoid this dilemma for producing the renewable, bio-based hydrocarbon fuel precursors, such as microbial lipid. Efficient bioconversion of lignocellulose-based sugars into lipids is one of the critical parameters for industrial application. Therefore, the fed-batch cultivation, which is a common method used in industrial applications, was investigated to achieve a high cell density culture along with high lipid yield and productivity. In this study, several fed-batch strategies were explored to improve lipid production using lignocellulosic hydrolysates derived from corn stover. Compared to the batch culture giving a lipid yield of 0.19 g/g, the dissolved-oxygen-stat feeding mode increased the lipid yield to 0.23 g/g and the lipid productivity to 0.33 g/L/h. The pulse feeding mode further improved lipid productivity to 0.35 g/L/h and the yield to 0.24 g/g. However, the highest lipid yield (0.29 g/g) and productivity (0.4 g/L/h) were achieved using an automated onlinemore » sugar control feeding mode, which gave a dry cell weight of 54 g/L and lipid content of 59 % (w/w). The major fatty acids of the lipid derived from lignocellulosic hydrolysates were predominately palmitic acid and oleic acid, which are similar to those of conventional oilseed plants. Our results suggest that the fed-batch feeding strategy can strongly influence the lipid production. Lastly, the online sugar control feeding mode was the most appealing strategy for high cell density, lipid yield, and lipid productivity using lignocellulosic hydrolysates as the sole carbon source.« less

Authors:
 [1];  [2];  [2];  [2];  [3];  [2]
  1. Xi'an Jiaotong Univ., Xi'an (China); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States); KBI Biopharma, Boulder, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1263902
Report Number(s):
NREL/JA-5100-65376
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; lignocellulosic hydrolysates; lipid production; fed-batch feeding strategy; high cell density culture; Rhodosporidium toruloides; automated online sugar control system

Citation Formats

Fei, Qiang, O'Brien, Marykate, Nelson, Robert, Chen, Xiaowen, Lowell, Andrew, and Dowe, Nancy. Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. United States: N. p., 2016. Web. doi:10.1186/s13068-016-0542-x.
Fei, Qiang, O'Brien, Marykate, Nelson, Robert, Chen, Xiaowen, Lowell, Andrew, & Dowe, Nancy. Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. United States. doi:10.1186/s13068-016-0542-x.
Fei, Qiang, O'Brien, Marykate, Nelson, Robert, Chen, Xiaowen, Lowell, Andrew, and Dowe, Nancy. Thu . "Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source". United States. doi:10.1186/s13068-016-0542-x. https://www.osti.gov/servlets/purl/1263902.
@article{osti_1263902,
title = {Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source},
author = {Fei, Qiang and O'Brien, Marykate and Nelson, Robert and Chen, Xiaowen and Lowell, Andrew and Dowe, Nancy},
abstractNote = {Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for fermentation-based bioprocesses are obtained from agricultural commodities that are used as foodstuff for human beings. Lignocellulose-derived sugars as the non-food, green, and sustainable alternative carbon sources have great potential to avoid this dilemma for producing the renewable, bio-based hydrocarbon fuel precursors, such as microbial lipid. Efficient bioconversion of lignocellulose-based sugars into lipids is one of the critical parameters for industrial application. Therefore, the fed-batch cultivation, which is a common method used in industrial applications, was investigated to achieve a high cell density culture along with high lipid yield and productivity. In this study, several fed-batch strategies were explored to improve lipid production using lignocellulosic hydrolysates derived from corn stover. Compared to the batch culture giving a lipid yield of 0.19 g/g, the dissolved-oxygen-stat feeding mode increased the lipid yield to 0.23 g/g and the lipid productivity to 0.33 g/L/h. The pulse feeding mode further improved lipid productivity to 0.35 g/L/h and the yield to 0.24 g/g. However, the highest lipid yield (0.29 g/g) and productivity (0.4 g/L/h) were achieved using an automated online sugar control feeding mode, which gave a dry cell weight of 54 g/L and lipid content of 59 % (w/w). The major fatty acids of the lipid derived from lignocellulosic hydrolysates were predominately palmitic acid and oleic acid, which are similar to those of conventional oilseed plants. Our results suggest that the fed-batch feeding strategy can strongly influence the lipid production. Lastly, the online sugar control feeding mode was the most appealing strategy for high cell density, lipid yield, and lipid productivity using lignocellulosic hydrolysates as the sole carbon source.},
doi = {10.1186/s13068-016-0542-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {2016},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

Figures / Tables:

Table 1 Table 1: Comparison of lipid production by various oleaginous yeasts using different carbon substrates in batch and fed-batch cultures

Save / Share:

Works referenced in this record:

High-cell-density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source
journal, June 1996

  • Meesters, P. A. E. P.; Huijberts, G. N. M.; Eggink, G.
  • Applied Microbiology and Biotechnology, Vol. 45, Issue 5
  • DOI: 10.1007/s002530050731

Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges
journal, January 2015


High cell density fed-batch fermentations for lipase production: feeding strategies and oxygen transfer
journal, March 2013

  • Salehmin, M. N. I.; Annuar, M. S. M.; Chisti, Y.
  • Bioprocess and Biosystems Engineering, Vol. 36, Issue 11
  • DOI: 10.1007/s00449-013-0943-1

Lipid production in batch and fed-batch cultures of Rhodosporidium toruloidesfrom 5 and 6 carbon carbohydrates
journal, May 2012

  • Wiebe, Marilyn G.; Koivuranta, Kari; Penttilä, Merja
  • BMC Biotechnology, Vol. 12, Issue 1
  • DOI: 10.1186/1472-6750-12-26

Can biofuels be a solution to climate change? The implications of land use change-related emissions for policy
journal, February 2011

  • Khanna, Madhu; Crago, Christine L.; Black, Mairi
  • Interface Focus, Vol. 1, Issue 2
  • DOI: 10.1098/rsfs.2010.0016

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum
journal, February 2012


Competitiveness of Brazilian sugarcane ethanol compared to US corn ethanol
journal, November 2010


Valorisation of side streams from wheat milling and confectionery industries for consolidated production and extraction of microbial lipids
journal, May 2016


Acid-catalyzed algal biomass pretreatment for integrated lipid and carbohydrate-based biofuels production
journal, January 2015

  • Laurens, L. M. L.; Nagle, N.; Davis, R.
  • Green Chemistry, Vol. 17, Issue 2
  • DOI: 10.1039/C4GC01612B

A multivariate study of the correlation between tocopherol content and fatty acid composition in vegetable oils
journal, April 1997

  • Kamal-Eldin, Afaf; Andersson, Roger
  • Journal of the American Oil Chemists' Society, Vol. 74, Issue 4
  • DOI: 10.1007/s11746-997-0093-1

Production of poly(3-hydroxybutyric acid) by fed-batch culture ofAlcaligenes eutrophus with glucose concentration control
journal, April 1994

  • Kim, Beom Soo; Lee, Seung Chul; Lee, Sang Yup
  • Biotechnology and Bioengineering, Vol. 43, Issue 9
  • DOI: 10.1002/bit.260430908

High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat
journal, April 2004

  • Kim, Beom Soo; Lee, Seung Chul; Lee, Sang Yup
  • Bioprocess and Biosystems Engineering, Vol. 26, Issue 3
  • DOI: 10.1007/s00449-003-0347-8

Microbial lipid production by oleaginous yeast Cryptococcus sp. in the batch cultures using corncob hydrolysate as carbon source
journal, January 2015


Relating the cetane number of biodiesel fuels to their fatty acid composition: A critical study
journal, April 2009

  • Gopinath, A.; Puhan, S.; Nagarajan, G.
  • Proceedings of the Institution of Mechanical Engineers, Part D: Journal of Automobile Engineering, Vol. 223, Issue 4
  • DOI: 10.1243/09544070JAUTO950

Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels
journal, January 2014


Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeastLipomyces starkeyi
journal, May 2008

  • Zhao, Xin; Kong, Xiangli; Hua, Yanyan
  • European Journal of Lipid Science and Technology, Vol. 110, Issue 5
  • DOI: 10.1002/ejlt.200700224

Trends in biotechnological production of fuel ethanol from different feedstocks
journal, September 2008


Analysis of biofuels production from sugar based on three criteria: Thermodynamics, bioenergetics, and product separation
journal, January 2011

  • Huang, Wei-Dong; Percival Zhang, Y. -H.
  • Energy Environ. Sci., Vol. 4, Issue 3
  • DOI: 10.1039/C0EE00069H

Microbial lipid production by Rhodosporidium toruloides under sulfate-limited conditions
journal, January 2011


Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010


Converting crude glycerol derived from yellow grease to lipids through yeast fermentation
journal, October 2010


Economic impact of total solids loading on enzymatic hydrolysis of dilute acid pretreated corn stover
journal, April 2010

  • Humbird, David; Mohagheghi, Ali; Dowe, Nancy
  • Biotechnology Progress, Vol. 26, Issue 5
  • DOI: 10.1002/btpr.441

Influence of nitrogen and iron limitations on lipid production by Cryptococcus curvatus grown in batch and fed-batch culture
journal, May 1996


Lipid production from Jerusalem artichoke by Rhodosporidium toruloides Y4
journal, March 2010

  • Zhao, Xin; Wu, Siguo; Hu, Cuimin
  • Journal of Industrial Microbiology & Biotechnology, Vol. 37, Issue 6
  • DOI: 10.1007/s10295-010-0704-y

Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification
journal, February 2012

  • Laurens, Lieve M. L.; Quinn, Matthew; Van Wychen, Stefanie
  • Analytical and Bioanalytical Chemistry, Vol. 403, Issue 1
  • DOI: 10.1007/s00216-012-5814-0

High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose
journal, April 2015


Microbial and algal oils: Do they have a future for biodiesel or as commodity oils?
journal, July 2008


Effect of biodiesel unsaturated fatty acid on combustion characteristics of a DI compression ignition engine
journal, August 2010


Zymomonas Mobilis —Science and Industrial Application
journal, January 1993

  • Doelle, Horst W.; Kirk, Lisa; Crittenden, Ross
  • Critical Reviews in Biotechnology, Vol. 13, Issue 1
  • DOI: 10.3109/07388559309069198

Effect of feeding strategies on lipid production by Lipomyces starkeyi
journal, April 2014


Exploring low-cost carbon sources for microbial lipids production by fed-batch cultivation of Cryptococcus albidus
journal, June 2011

  • Fei, Qiang; Chang, Ho Nam; Shang, Longan
  • Biotechnology and Bioprocess Engineering, Vol. 16, Issue 3
  • DOI: 10.1007/s12257-010-0370-y

The control of fed-batch fermentation processes—A survey
journal, November 1987


Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters
journal, June 2005


Cultivation and lipid production of yeast Cryptococcus curvatus using pretreated waste active sludge supernatant
journal, May 2013


Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
journal, January 2010

  • Steen, Eric J.; Kang, Yisheng; Bokinsky, Gregory
  • Nature, Vol. 463, Issue 7280, p. 559-562
  • DOI: 10.1038/nature08721

The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production
journal, February 2011


Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass
journal, April 2016


l-lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies
journal, June 2006


Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions
journal, July 2015

  • Rakicka, Magdalena; Lazar, Zbigniew; Dulermo, Thierry
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0286-z

Prospects and potential of fatty acid methyl esters of some non-traditional seed oils for use as biodiesel in India
journal, October 2005


Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies
journal, August 2010

  • Zhao, Xin; Hu, Cuimin; Wu, Siguo
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 5
  • DOI: 10.1007/s10295-010-0808-4

High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
journal, August 2007


Lipid production from hydrolysate of cassava starch by Rhodosporidium toruloides 21167 for biodiesel making
journal, October 2012


Improving lipid production from bagasse hydrolysate with Trichosporon fermentans by response surface methodology
journal, February 2012


    Works referencing / citing this record:

    Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
    journal, June 2019

    • Tiukova, Ievgeniia A.; Brandenburg, Jule; Blomqvist, Johanna
    • Biotechnology for Biofuels, Vol. 12, Issue 1
    • DOI: 10.1186/s13068-019-1478-8

    Optimization of lipid production potential of oleaginous yeast by response surface methodology cultivated in agro-industrial waste
    journal, June 2018

    • Mirza, S.; Siddique, S.; Qamer, H. M.
    • International Journal of Environmental Science and Technology, Vol. 16, Issue 7
    • DOI: 10.1007/s13762-018-1878-5

    Optimization of lipid production potential of oleaginous yeast by response surface methodology cultivated in agro-industrial waste
    journal, June 2018

    • Mirza, S.; Siddique, S.; Qamer, H. M.
    • International Journal of Environmental Science and Technology, Vol. 16, Issue 7
    • DOI: 10.1007/s13762-018-1878-5

    Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
    journal, June 2019

    • Tiukova, Ievgeniia A.; Brandenburg, Jule; Blomqvist, Johanna
    • Biotechnology for Biofuels, Vol. 12, Issue 1
    • DOI: 10.1186/s13068-019-1478-8

    Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate
    journal, April 2020


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.