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Title: Mechanism of lignin inhibition of enzymatic biomass deconstruction

The conversion of plant biomass to ethanol via enzymatic cellulose hydrolysis offers a potentially sustainable route to biofuel production. However, the inhibition of enzymatic activity in pretreated biomass by lignin severely limits the efficiency of this process. By performing atomic-detail molecular dynamics simulation of a biomass model containing cellulose, lignin, and cellulases (TrCel7A), we elucidate detailed lignin inhibition mechanisms. We find that lignin binds preferentially both to the elements of cellulose to which the cellulases also preferentially bind (the hydrophobic faces) and also to the specific residues on the cellulose-binding module of the cellulase that are critical for cellulose binding of TrCel7A (Y466, Y492, and Y493). In conclusion, lignin thus binds exactly where for industrial purposes it is least desired, providing a simple explanation of why hydrolysis yields increase with lignin removal.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [2] ;  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics; Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics; Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725; FG02-97ER25308
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Cellulose crystallinity; Cel7A; Lignin; Biofuel; biofuel; lignin; cellulose crystallinity
OSTI Identifier:
1260590
Alternate Identifier(s):
OSTI ID: 1327644

Vermaas, Josh V., Petridis, Loukas, Qi, Xianghong, Schulz, Roland, Lindner, Benjamin, and Smith, Jeremy. C.. Mechanism of lignin inhibition of enzymatic biomass deconstruction. United States: N. p., Web. doi:10.1186/s13068-015-0379-8.
Vermaas, Josh V., Petridis, Loukas, Qi, Xianghong, Schulz, Roland, Lindner, Benjamin, & Smith, Jeremy. C.. Mechanism of lignin inhibition of enzymatic biomass deconstruction. United States. doi:10.1186/s13068-015-0379-8.
Vermaas, Josh V., Petridis, Loukas, Qi, Xianghong, Schulz, Roland, Lindner, Benjamin, and Smith, Jeremy. C.. 2015. "Mechanism of lignin inhibition of enzymatic biomass deconstruction". United States. doi:10.1186/s13068-015-0379-8. https://www.osti.gov/servlets/purl/1260590.
@article{osti_1260590,
title = {Mechanism of lignin inhibition of enzymatic biomass deconstruction},
author = {Vermaas, Josh V. and Petridis, Loukas and Qi, Xianghong and Schulz, Roland and Lindner, Benjamin and Smith, Jeremy. C.},
abstractNote = {The conversion of plant biomass to ethanol via enzymatic cellulose hydrolysis offers a potentially sustainable route to biofuel production. However, the inhibition of enzymatic activity in pretreated biomass by lignin severely limits the efficiency of this process. By performing atomic-detail molecular dynamics simulation of a biomass model containing cellulose, lignin, and cellulases (TrCel7A), we elucidate detailed lignin inhibition mechanisms. We find that lignin binds preferentially both to the elements of cellulose to which the cellulases also preferentially bind (the hydrophobic faces) and also to the specific residues on the cellulose-binding module of the cellulase that are critical for cellulose binding of TrCel7A (Y466, Y492, and Y493). In conclusion, lignin thus binds exactly where for industrial purposes it is least desired, providing a simple explanation of why hydrolysis yields increase with lignin removal.},
doi = {10.1186/s13068-015-0379-8},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 8,
place = {United States},
year = {2015},
month = {12}
}

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