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Title: Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity

Abstract

Stomata, epidermal valves facilitating plant–atmosphere gas exchange, represent a powerful model for understanding cell fate and pattern in plants. Core basic helix–loop–helix (bHLH) transcription factors regulating stomatal development were identified in Arabidopsis , but this dicot’s developmental pattern and stomatal morphology represent only one of many possibilities in nature. Here, using unbiased forward genetic screens, followed by analysis of reporters and engineered mutants, we show that stomatal initiation in the grass Brachypodium distachyon uses orthologs of stomatal regulators known from Arabidopsis but that the function and behavior of individual genes, the relationships among genes, and the regulation of their protein products have diverged. Our results highlight ways in which a kernel of conserved genes may be alternatively wired to produce diversity in patterning and morphology and suggest that the stomatal transcription factor module is a prime target for breeding or genome modification to improve plant productivity.

Authors:
; ; ; ORCiD logo;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1260426
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 29; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English

Citation Formats

Raissig, Michael T., Abrash, Emily, Bettadapur, Akhila, Vogel, John P., and Bergmann, Dominique C.. Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity. United States: N. p., 2016. Web. doi:10.1073/pnas.1606728113.
Raissig, Michael T., Abrash, Emily, Bettadapur, Akhila, Vogel, John P., & Bergmann, Dominique C.. Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity. United States. doi:10.1073/pnas.1606728113.
Raissig, Michael T., Abrash, Emily, Bettadapur, Akhila, Vogel, John P., and Bergmann, Dominique C.. Tue . "Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity". United States. doi:10.1073/pnas.1606728113.
@article{osti_1260426,
title = {Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity},
author = {Raissig, Michael T. and Abrash, Emily and Bettadapur, Akhila and Vogel, John P. and Bergmann, Dominique C.},
abstractNote = {Stomata, epidermal valves facilitating plant–atmosphere gas exchange, represent a powerful model for understanding cell fate and pattern in plants. Core basic helix–loop–helix (bHLH) transcription factors regulating stomatal development were identified in Arabidopsis , but this dicot’s developmental pattern and stomatal morphology represent only one of many possibilities in nature. Here, using unbiased forward genetic screens, followed by analysis of reporters and engineered mutants, we show that stomatal initiation in the grass Brachypodium distachyon uses orthologs of stomatal regulators known from Arabidopsis but that the function and behavior of individual genes, the relationships among genes, and the regulation of their protein products have diverged. Our results highlight ways in which a kernel of conserved genes may be alternatively wired to produce diversity in patterning and morphology and suggest that the stomatal transcription factor module is a prime target for breeding or genome modification to improve plant productivity.},
doi = {10.1073/pnas.1606728113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 29,
volume = 113,
place = {United States},
year = {2016},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1073/pnas.1606728113

Citation Metrics:
Cited by: 11 works
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Works referenced in this record:

A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
journal, September 2003

  • Curtis, Mark D.; Grossniklaus, Ueli
  • Plant Physiology, Vol. 133, Issue 2, p. 462-469
  • DOI: 10.1104/pp.103.027979