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Title: Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level

We probed the lipid expression dynamics of the oleaginous yeast Yarrowia Lipolytica. We observed that neutral lipid expression is sporadic. By performing single-cell analysis, we found that such noise emanates from the metabolic reaction level. Our results provide an alternative insight into the regulation and phenotypic variability of lipogenesis.
Authors:
 [1] ;  [2] ;  [2]
  1. Univ. of Idaho, Moscow, ID (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering
Publication Date:
Report Number(s):
PNNL-SA-110702
Journal ID: ISSN 2045-2322; KP1704020
Grant/Contract Number:
AC05-76RL01830; SC0008744
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biophysical methods; metabolic engineering
OSTI Identifier:
1259946

Vasdekis, A. E., Silverman, A. M., and Stephanopoulos, G.. Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level. United States: N. p., Web. doi:10.1038/srep17689.
Vasdekis, A. E., Silverman, A. M., & Stephanopoulos, G.. Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level. United States. doi:10.1038/srep17689.
Vasdekis, A. E., Silverman, A. M., and Stephanopoulos, G.. 2015. "Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level". United States. doi:10.1038/srep17689. https://www.osti.gov/servlets/purl/1259946.
@article{osti_1259946,
title = {Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level},
author = {Vasdekis, A. E. and Silverman, A. M. and Stephanopoulos, G.},
abstractNote = {We probed the lipid expression dynamics of the oleaginous yeast Yarrowia Lipolytica. We observed that neutral lipid expression is sporadic. By performing single-cell analysis, we found that such noise emanates from the metabolic reaction level. Our results provide an alternative insight into the regulation and phenotypic variability of lipogenesis.},
doi = {10.1038/srep17689},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {2015},
month = {12}
}