Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level
We probed the lipid expression dynamics of the oleaginous yeast Yarrowia Lipolytica. We observed that neutral lipid expression is sporadic. By performing single-cell analysis, we found that such noise emanates from the metabolic reaction level. Our results provide an alternative insight into the regulation and phenotypic variability of lipogenesis.
- Authors:
-
[1]
; [2]
; [2]
- Univ. of Idaho, Moscow, ID (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering
- Publication Date:
- Report Number(s):
- PNNL-SA-110702
Journal ID: ISSN 2045-2322; KP1704020
- Grant/Contract Number:
- AC05-76RL01830; SC0008744
- Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Research Org:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org:
- USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biophysical methods; metabolic engineering
- OSTI Identifier:
- 1259946
- Free Publicly Accessible Full Text
-
Accepted Manuscript (DOE)
- Publisher's Version of Record
- https://doi.org/10.1038/srep17689